Physical characterization and analysis of tissue inflammatory response of the combination of hydroxyapatite gypsum puger and tapioca starch as a scaffold material
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Background: Cases of bone damage in the oral cavity are high, up to 70% of which consist of cases of fracture, tooth extraction, tumor, and mandibular resection. The high number of cases of bone damage will cause the need for bone graft material to increase. The bone graft material that we have developed is a combination of hydroxyapatite gypsum puger (HAGP) and tapioca starch (TS) scaffold. Purpose: This study analyzes the physical characterization and tissue inflammatory response of the combination of HAGP+TS as a scaffold for bone graft material. Methods: Eighteen Wistar rats were used. HAGP+TS were installed into the molar 1 socket for 7 and 14 days. First, HAGP was evaluated using XRF and SEM before setting up the in vivo experiment. A blood sample was drawn and then tested for TNF-α levels using ELISA. Results: The XRF revealed that the main constituents of hydroxyapatite were Ca and P. Next, SEM characterization on the HAGP+TS showed an average pore size of 112.42 µm2, which is beneficial for cell activity to grow as new bone tissue. In addition, TNF-α on days 7 and 14 on the HAGP+TS scaffold did not elicit an inflammatory response. Conclusion: The combination of HAGP+TS contains a high amount of Ca and also has excellent interconnectivity between pores. It also does not trigger an inflammatory response in the tissue; therefore, it is a good candidate as an alternative bone graft material.
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Kheirallah M, Almeshaly H. Present strategies for critical bone defects regeneration. Oral Heal Case Reports. 2016; 2(3): 127. doi: https://doi.org/10.4172/2471-8726.1000127
Mattiola A, Bosshardt D, Schmidlin P. The rigid-shield technique: a new contour and clot stabilizing method for ridge preservation. Dent J. 2018; 6(2): 21. doi: https://doi.org/10.3390/dj6020021
Alkaabi SA, Alsabri GA, NatsirKalla DS, Alavi SA, Mueller WEG, Forouzanfar T, Helder MN. A systematic review on regenerative alveolar graft materials in clinical trials: Risk of bias and meta-analysis. J Plast Reconstr Aesthet Surg. 2022; 75(1): 356–65. doi: https://doi.org/10.1016/j.bjps.2021.08.026
Kolk A, Handschel J, Drescher W, Rothamel D, Kloss F, Blessmann M, Heiland M, Wolff K-D, Smeets R. Current trends and future perspectives of bone substitute materials - from space holders to innovative biomaterials. J Craniomaxillofac Surg. 2012; 40(8): 706–18. doi: https://doi.org/10.1016/j.jcms.2012.01.002
Tal H, Artzi Z, Kolerman R, Beitlitum I, Goshe G. Augmentation and preservation of the alveolar process and alveolar ridge of bone. In: Bone Regeneration. InTech; 2012. p. 139–84. doi: https://doi.org/10.5772/33839
Naini A, Sudiana IK, Rubianto M, Ferdiansyah, Mufti N. Characterization and degradation of hydroxyapatite gypsum puger (HAGP) freeze dried scaffold as a graft material for preservation of the alveolar bone socket. J Int Dent Med Res. 2018; 11(2): 532–6. pdf: http://www.jidmr.com/journal/wp-content/uploads/2018/09/29D17_490-Layout.pdf
Naini A, Sudiana IK, Rubianto M, Kresnoadi U, Latief FDE. Effects of hydroxyapatite gypsum puger scaffold applied to rat alveolar bone sockets on osteoclasts, osteoblasts and the trabecular bone area. Dent J (Majalah Kedokt Gigi). 2019; 52(1): 13–7. doi: https://doi.org/10.20473/j.djmkg.v52.i1.p13-17
Tripathi G, Basu B. A porous hydroxyapatite scaffold for bone tissue engineering: Physico-mechanical and biological evaluations. Ceram Int. 2012; 38(1): 341–9. doi: https://doi.org/10.1016/j.ceramint.2011.07.012
Ndubuisi ND, Chidiebere ACU. Cyanide in cassava: a review. Int J Genomics Data Min. 2018; 2: 118. doi: https://doi.org/10.29011/2577-0616.000118
Bucholz RW. Rockwood and Green’s fractures in adults. 7th ed. Vol. 1. Lippincott Williams & Wilkins; 2010. p. 113–4. web: https://books.google.co.id/books?id=e6KJtgEACAAJ&hl=id&source=gbs_book_other_versions
Baratawidjaja KG, Rengganis I. Imunologi dasar. 11th ed. Jakarta: Fakultas Kedokteran Universitas Indonesia; 2014. p. 860. web: https://lib.ui.ac.id/detail?id=20417471
Naini A, Rachmawati D. Composition analysis of calcium and sulfur on gypsum at the Puger District Jember Regency as an alternative gypsum dental material. Dentika Dent J. 2010; 15(2): 179–83. doi: https://doi.org/10.32734/DENTIKA.V15I2.1939
Choi AH, Ben-Nissan B, Matinlinna JP, Conway RC. Current perspectives: calcium phosphate nanocoatings and nanocomposite coatings in dentistry. J Dent Res. 2013; 92(10): 853–9. doi: https://doi.org/10.1177/0022034513497754
Remya NS, Syama S, Gayathri V, Varma HK, Mohanan P V. An in vitro study on the interaction of hydroxyapatite nanoparticles and bone marrow mesenchymal stem cells for assessing the toxicological behaviour. Colloids Surf B Biointerfaces. 2014; 117: 389–97. doi: https://doi.org/10.1016/j.colsurfb.2014.02.004
Ramadoss P, Subha V, Kirubanandan S. Gelatin-silk fibroin composite scaffold as a potential skin graft material. J Mater Sci Surf Eng. 2018; 6(2): 761–6. pdf: https://www.jmsse.in/files/621_Preethi_Ramadoss_et_al.PDF
Qi Y, Wang H, Wei K, Yang Y, Zheng R-Y, Kim I, Zhang K-Q. A review of structure construction of silk fibroin biomaterials from single structures to multi-level structures. Int J Mol Sci. 2017; 18(3): 237. doi: https://doi.org/10.3390/ijms18030237
Kaviani Z, Zamanian A. Effect of nanohydroxyapatite addition on the pore morphology and mechanical properties of freeze cast hydroxyapatite scaffolds. Procedia Mater Sci. 2015; 11: 190–5. doi: https://doi.org/10.1016/j.mspro.2015.11.102
Khan Y, Yaszemski MJ, Mikos AG, Laurencin CT. Tissue engineering of bone: material and matrix considerations. J Bone Joint Surg Am. 2008; 90(Suppl 1): 36–42. doi: https://doi.org/10.2106/JBJS.G.01260
Chocholata P, Kulda V, Babuska V. Fabrication of scaffolds for bone-tissue regeneration. Materials (Basel). 2019; 12(4): 568. doi: https://doi.org/10.3390/ma12040568
Ariani MD, Matsuura A, Hirata I, Kubo T, Kato K, Akagawa Y. New development of carbonate apatite-chitosan scaffold based on lyophilization technique for bone tissue engineering. Dent Mater J. 2013; 32(2): 317–25. doi: https://doi.org/10.4012/dmj.2012-257
Abbas A, Lichtman A, Pillai S. Cellular and molecular immunology. 9th ed. Philadelphia: Elsevier; 2016. p. 359–81. https://www.elsevier.com/books/cellular-and-molecular-immunology/abbas/978-0-323-47978-3
Cardemil C, Elgali I, Xia W, Emanuelsson L, Norlindh B, Omar O, Thomsen P. Strontium-doped calcium phosphate and hydroxyapatite granules promote different inflammatory and bone remodelling responses in normal and ovariectomised rats. PLoS One. 2013; 8(12): e84932. doi: https://doi.org/10.1371/journal.pone.0084932
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