Immunohistochemical differential expression of p16 proteins in follicular type and plexiform type ameloblastoma
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Background: Differences in histopathological features that describe the growth mechanism and biological behaviour of follicular and plexiform ameloblastomas are associated with benign, aggressive and destructive tumour markers. p16 has inhibitory interactions between cyclin D and CDK 4/6 to block the cell cycle and alterations related to severity. Purpose: This study intends to evaluate and determine differential expressions of p16 protein in follicular and plexiform ameloblastomas. Methods: This is a descriptive analytics study. A total of 21 specimens consisting of follicular and plexiform ameloblastomas and healthy gingiva tissues as the negative control were examined using the immunohistochemistry assay. The analysis of p16 protein expression was interpreted by immunoreactive scoring. Statistical analysis was conducted using SPSS software with the Mann–Whitney test. A p-value <0.05 shows the significance of the change in expression. Results: An increased expression of p16 protein was found in the follicular ameloblastoma type (2.13 ± 1.808) and the plexiform type (4.44 ± 2.506) in comparison to the negative control group (0 ± 0). The increase of p16 expression in the follicular and plexiform ameloblastomas was significant compared to the negative control group (p-value <0.05); however, there was no significant difference between either type of ameloblastoma (p-value >0.05). Conclusion: The highest intensity of p16 protein expression was found in the plexiform type, even though it was not significantly different from the follicular type ameloblastoma.
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Gomes CC, de Sousa SF, Gomez RS. Craniopharyngiomas and odontogenic tumors mimic normal odontogenesis and share genetic mutations, histopathologic features, and molecular pathways activation. Oral Surg Oral Med Oral Pathol Oral Radiol. 2019; 127(3): 231–6. doi: https://doi.org/10.1016/j.oooo.2018.11.004
Staines KS, Crighton A. Benign oral and dental disease. In: Watkinson JC, Clarke RW, editors. Scott-Brown's Otorhinolaryngology and Head and Neck Surgery. 8th ed. Boca Raton: CRC Press; 2018. p. 699–718. doi: https://doi.org/10.1201/9780203731000
Speight PM, Takata T. New tumour entities in the 4th edition of the World Health Organization classification of head and neck tumours: odontogenic and maxillofacial bone tumours. Virchows Arch. 2018; 472(3): 331–9. doi: https://doi.org/10.1007/s00428-017-2182-3
Doroy GAR, Gelbolingo N. Primary intraosseous carcinoma of the mandible: a case report. Philipp J Otolaryngol Head Neck Surg. 2020; 35(1): 56–9. doi: https://doi.org/10.32412/pjohns.v35i1.1283
Dean KE. A radiologist's guide to teeth: An imaging review of dental anatomy, nomenclature, trauma, infection, and tumors. Neurographics. 2020; 10(5): 302–18. doi: https://doi.org/10.3174/ng.2000024
Schwartz JL, Sroussi H. Genomic foundation for medical and oral disease translation to clinical assessment. In: Sonis ST, Villa A, editors. Translational Systems Medicine and Oral Disease. St. Louis: Elsevier; 2020. p. 17–92. doi: https://doi.org/10.1016/B978-0-12-813762-8.00003-7
Du Y, Cheng Y, Su G. The essential role of tumor suppressor gene ING4 in various human cancers and non-neoplastic disorders. Biosci Rep. 2019; 39(1): BSR20180773. doi: https://doi.org/10.1042/BSR20180773
Sánchez-Martínez C, Gelbert LM, Lallena MJ, de Dios A. Cyclin dependent kinase (CDK) inhibitors as anticancer drugs. Bioorg Med Chem Lett. 2015; 25(17): 3420–35. doi: https://doi.org/10.1016/j.bmcl.2015.05.100
Tripathy D, Bardia A, Sellers WR. Ribociclib (LEE011): Mechanism of action and clinical impact of this selective cyclin-dependent kinase 4/6 inhibitor in various solid tumors. Clin Cancer Res. 2017; 23(13): 3251–62. doi: https://doi.org/10.1158/1078-0432.CCR-16-3157
Shan M, Zhang X, Liu X, Qin Y, Liu T, Liu Y, Wang J, Zhong Z, Zhang Y, Geng J, Pang D. P16 and p53 play distinct roles in different subtypes of breast cancer. PLoS One. 2013; 8(10): e76408. doi: https://doi.org/10.1371/journal.pone.0076408
Dadhania V, Zhang M, Zhang L, Bondaruk J, Majewski T, Siefker-Radtke A, Guo CC, Dinney C, Cogdell DE, Zhang S, Lee S, Lee JG, Weinstein JN, Baggerly K, McConkey D, Czerniak B. Meta-analysis of the luminal and basal subtypes of bladder cancer and the identification of signature immunohistochemical markers for clinical use. EBioMedicine. 2016; 12: 105–17. doi: https://doi.org/10.1016/j.ebiom.2016.08.036
Moffitt RA, Marayati R, Flate EL, Volmar KE, Loeza SGH, Hoadley KA, Rashid NU, Williams LA, Eaton SC, Chung AH, Smyla JK, Anderson JM, Kim HJ, Bentrem DJ, Talamonti MS, Iacobuzio-Donahue CA, Hollingsworth MA, Yeh JJ. Virtual microdissection identifies distinct tumor- and stroma-specific subtypes of pancreatic ductal adenocarcinoma. Nat Genet. 2015; 47(10): 1168–78. doi: https://doi.org/10.1038/ng.3398
Razavi SM, Poursadeghi H, Aminzadeh A. Immunohistochemical comparison of cyclin D1 and P16 in odontogenic keratocyst and unicystic ameloblastoma. Dent Res J (Isfahan). 2013; 10(2): 180–3. doi: https://doi.org/10.4103/1735-3327.113336
Ogrodnik M, Salmonowicz H, Jurk D, Passos JF. Expansion and cell-cycle arrest: common denominators of cellular senescence. Trends Biochem Sci. 2019; 44(12): 996–1008. doi: https://doi.org/10.1016/j.tibs.2019.06.011
Canaud G, Bonventre J V. Cell cycle arrest and the evolution of chronic kidney disease from acute kidney injury. Nephrol Dial Transplant. 2015; 30(4): 575–83. doi: https://doi.org/10.1093/ndt/gfu230
Cadavid AMH, Araujo JP, Coutinho-Camillo CM, Bologna S, Junior CAL, Lourenço SV. Ameloblastomas: current aspects of the new WHO classification in an analysis of 136 cases. Surg Exp Pathol. 2019; 2(1): 17. doi: https://doi.org/10.1186/s42047-019-0041-z
Verneuil A, Sapp P, Huang C, Abemayor E. Malignant ameloblastoma: classification, diagnostic, and therapeutic challenges. Am J Otolaryngol. 2002; 23(1): 44–8. doi: https://doi.org/10.1053/ajot.2002.28769
Surowiak P, Materna V, Maciejczyk A, Pudelko M, Suchocki S, Kedzia W, Nowak-Markwitz E, Dumanska M, Spaczynski M, Zabel M, Dietel M, Lage H. Decreased expression of p16 in ovarian cancers represents an unfavourable prognostic factor. Histol Histopathol. 2008; 23(5): 531–8. doi: https://doi.org/10.14670/HH-23.531
Wang Y, Chen S, Yan Z, Pei M. A prospect of cell immortalization combined with matrix microenvironmental optimization strategy for tissue engineering and regeneration. Cell Biosci. 2019; 9(1): 7. doi: https://doi.org/10.1186/s13578-018-0264-9
Lazăr CS, Åžovrea AS, Georgiu C, Crişan D, Mirescu ÅžC, Cosgarea M. Different patterns of p16INK4a immunohistochemical expression and their biological implications in laryngeal squamous cell carcinoma. Rom J Morphol Embryol. 2020; 61(3): 697–706. doi: https://doi.org/10.47162/RJME.61.3.08
Li M, Yang J, Liu K, Yang J, Zhan X, Wang L, Shen X, Chen J, Mao Z. p16 promotes proliferation in cervical carcinoma cells through CDK6-HuR-IL1A axis. J Cancer. 2020; 11(6): 1457–67. doi: https://doi.org/10.7150/jca.35479
Lee SK, Kim YS. Current concepts and occurrence of epithelial odontogenic tumors: I. Ameloblastoma and adenomatoid odontogenic tumor. Korean J Pathol. 2013; 47(3): 191–202. doi: https://doi.org/10.4132/KoreanJPathol.2013.47.3.191
Diniz MG, Guimarí£es BVA, Pereira NB, de Menezes GHF, Gomes CC, Gomez RS. DNA damage response activation and cell cycle dysregulation in infiltrative ameloblastomas: A proposed model for ameloblastoma tumor evolution. Exp Mol Pathol. 2017; 102(3): 391–5. doi: https://doi.org/10.1016/j.yexmp.2017.04.003
Boscolo-Rizzo P, Da Mosto MC, Rampazzo E, Giunco S, Del Mistro A, Menegaldo A, Baboci L, Mantovani M, Tirelli G, De Rossi A. Telomeres and telomerase in head and neck squamous cell carcinoma: from pathogenesis to clinical implications. Cancer Metastasis Rev. 2016; 35(3): 457–74. doi: https://doi.org/10.1007/s10555-016-9633-1
Merlin JPJ, Rupasinghe HPV, Dellaire G, Murphy K. Role of dietary antioxidants in p53-mediated cancer chemoprevention and tumor suppression. Oxid Med Cell Longev. 2021; 2021: 9924328. doi: https://doi.org/10.1155/2021/9924328
LaPak KM, Burd CE. The molecular balancing act of p16(INK4a) in cancer and aging. Mol Cancer Res. 2014; 12(2): 167–83. doi: https://doi.org/10.1158/1541-7786.MCR-13-0350
Pack LR, Daigh LH, Chung M, Meyer T. Clinical CDK4/6 inhibitors induce selective and immediate dissociation of p21 from cyclin D-CDK4 to inhibit CDK2. Nat Commun. 2021; 12(1): 3356. doi: https://doi.org/10.1038/s41467-021-23612-z
Ombiro EM, Kwena A, Melly E, Kamau T, Maiyoh GK. Genotypes and prevalence of high-risk human papillomavirus among patients diagnosed with head and neck cancer at Alexandria Cancer Centre. JCO Glob Oncol. 2020; 6(Supplement_1): 30–30. doi: https://doi.org/10.1200/GO.20.25000
Mahale S, Bharate SB, Manda S, Joshi P, Bharate SS, Jenkins PR, Vishwakarma RA, Chaudhuri B. Biphenyl-4-carboxylic acid [2-(1H-indol-3-yl)-ethyl]-methylamide (CA224), a nonplanar analogue of fascaplysin, inhibits Cdk4 and tubulin polymerization: evaluation of in vitro and in vivo anticancer activity. J Med Chem. 2014; 57(22): 9658–72. doi: https://doi.org/10.1021/jm5014743
Salari Fanoodi T, Motalleb G, Yegane Moghadam A, Talaee R. p21 gene expression evaluation in esophageal cancer patients. Gastrointest Tumors. 2015; 2(3): 144–64. doi: https://doi.org/10.1159/000441901
Kumamoto H, Kimi K, Ooya K. Detection of cell cycle-related factors in ameloblastomas. J Oral Pathol Med. 2001; 30(5): 309–15. doi: https://doi.org/10.1034/j.1600-0714.2001.300509.x
Artese L, Piattelli A, Rubini C, Goteri G, Perrotti V, Iezzi G, Piccirilli M, Carinci F. p16 expression in odontogenic tumors. Tumori. 2008; 94(5): 718–23. doi: https://doi.org/10.1177/030089160809400513
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