Demineralized dentin characteristics after application of Mauli banana stem gel
Downloads
Background: Demineralization of dentin is a condition of dissolving minerals in peritubular dentin due to exposure to acids that release hydroxyapatite ions. To prevent dentin demineralization, a therapeutic agent that can inhibit the dissolution ability of hydroxyapatite ions is needed. One therapeutic agent that can be used is the Mauli banana (MB) stem gel. Purpose: To observe the characteristics of dentin demineralization after the use of MB stem extract gel. Method: Mandibular incisor bovine teeth were demineralized with lactic acid pH 4.5 for 72 hours and then treated with 25%; 37.5%; 50%; and 62.5% MB gel and Chlorhexidine 2% for one minute. All samples were soaked in artificial saliva with 1 mg/ml saliva of collagenase enzyme for 24 hours. The characteristic of dentin demineralization was observed by using scanning electron microscope/electron dispersive X-ray spectroscopy (SEM-EDX). Results: The SEM image in the control, Chlorhexidine, 25%; 37.5%; 50%; and 62.5% MB gel groups showed dentinal tubules of about 3.67–4.94 µm; 3.55–4 µm; 4.18–5.6 µm; 2.28–2.86 µm; 3.29–3.81 µm; and 2.42–3.17 µm in size. The EDX test found carbon (C), nitrogen (N), oxygen (O), sodium (Na), phosphorous (P), chlorine (Cl), and calcium (Ca) in all groups. The one-way Analysis of Variance (ANOVA) test results showed significant differences in the levels of C, N, O, Cl, and Ca between all groups, while the Na showed no significant differences. Conclusion: The MB can inhibit the demineralization of bovine dentin based on the decrease in the size of the dentinal tubules and increasing the C, O, P, and Ca.
Downloads
Mulyawan I, Danudiningrat CP, Soesilawati P, Aulanni’am A, Yuliati A, Suroto H, Bramantoro T, Rizqiawan A, Moon S-Y. The characteristics of demineralized dentin material sponge as guided bone regeneration based on the FTIR and SEM-EDX tests. Eur J Dent. 2022; 16(4): 880–5. doi: https://doi.org/10.1055/s-0042-1743147
Astuti ND, Apriasari ML, Nahzi MYI. The effect of mauli banana (Musa acuminata) stem extract on macrophage in pulp inflamation (In vivo study in male Wistar rat (Rattusnovergicus) teeth). Dentino J Kedokt Gigi. 2018; 3(1): 37–42. web: https://ppjp.ulm.ac.id/journal/index.php/dentino/article/view/4616
Badan Penelitian dan Pengembangan Kesehatan. Laporan nasional riset kesehatan dasar 2018. Jakarta: Kementerian Kesehatan Republik Indonesia; 2018. p. 674. web: http://repository.bkpk.kemkes.go.id/3514/
Apriasari ML, Pramitha SR, Puspitasari D, Ernawati DS. Anti-inflammatory effect of Musa acuminata stem. Eur J Dent. 2020; 14(2): 294–8. doi: https://doi.org/10.1055/s-0040-1709944
Firouzmandi M, Shafiei F, Jowkar Z, Nazemi F. Effect of silver diamine fluoride and proanthocyanidin on mechanical properties of caries-affected dentin. Eur J Dent. 2019; 13(2): 255–60. doi: https://doi.org/10.1055/s-0039-1693237
Mitthra S, Narasimhan M, Shakila R, Anuradha B. Demineralization – An overview of the mechanism and causative agents. Indian J Forensic Med Toxicol. 2020; 14(4): 1173–8. doi: https://doi.org/10.37506/ijfmt.v14i4.11679
Sabel N, Robertson A, Nietzsche S, Norén JG. Demineralization of enamel in primary second molars related to properties of the enamel. Sci World J. 2012; 2012: 1–8. doi: https://doi.org/10.1100/2012/587254
Younis SH, Obeid RF, Ammar MM. Subsurface enamel remineralization by Lyophilized Moringa leaf extract loaded varnish. Heliyon. 2020; 6(9): e05054. doi: https://doi.org/10.1016/j.heliyon.2020.e05054
Khunkar S, Hariri I, Alsayed E, Linjawi A, Khunkar S, Islam S, Bakhsh TA, Nakashima S. Inhibitory effect of Salvadora persica extract (Miswak) on collagen degradation in demineralized dentin: In vitro study. J Dent Sci. 2021; 16(1): 208–13. doi: https://doi.org/10.1016/j.jds.2020.05.025
Puspitasari D, Herda E, Soufyan A. Effect of 2% chlorhexidine gluconate on the degradation of resin composite–dentin bond strength when using self-etch adhesive systems. Int J Appl Pharm. 2018; 9(Special Issue 2): 45–50. doi: https://doi.org/10.22159/ijap.2017.v9s2.12
Maske TT, Kuper NK, Cenci MS, Huysmans M-CDNJM. Chlorhexidine, a matrix metalloproteinase inhibitor and the development of secondary caries wall lesions in a microcosm biofilm model. Caries Res. 2019; 53(1): 107–17. doi: https://doi.org/10.1159/000490195
Iovan A, Stoleriu S, Ghiorghe CA, Pancu G, Topoliceanu C, Moldovanu A, Iovan G, Andrian S. Effect of silver diamine fluoride and potassium iodide on artificial root caries subjected to demineralization. Rom J Oral Rehabil. 2021; 13(3): 82–91. web: https://rjor.ro/effect-of-silver-diamine-fluoride-and-potassium-iodide-on-artificial-root-caries-subjected-to-demineralization/
Nindia A, Aspriyanto D, Apriasari ML, Rizky S. The toxicity of mauli banana (Musa Acuminata) stem water extract on bone marrow mesenchymal stem cell in vitro. Int J Appl Pharm. 2019; 11(1): 184–6. doi: https://doi.org/10.22159/ijap.2019.v11s1.16762
Apriasari ML, Fadhilah A, Carabelly AN. Aktivitas antibakteri ekstrak metanol batang pisang mauli (Musa sp) terhadap Streptococcus mutans Antibacterial activity of methanol extract of mauli banana (Musa sp)stems against Streptococcus mutans. J Dentofasial. 2013; 12(3): 148–51. web: https://jdmfs.org/index.php/jdmfs/article/view/369
Carabelly AN, Salsabila Karno DA, Erlita I, Trianuanty AP. Viability of dual-species biofilm of Streptococcus mutans and Lactobacillus acidophilus after application of mauli banana stem gel. Dentino J Kedokt Gigi. 2022; 7(1): 55–61. doi: https://doi.org/10.20527/dentino.v7i1.13104
Zeng X, Ding X, Pu Y, Jiang H, Du Z, Jiang W. Banana condensed tannins scavenge glyphosate in aqueous solution through non-covalent interactions. LWT - Food Sci Technol. 2020; 131(March): 109697. doi: https://doi.org/10.1016/j.lwt.2020.109697
Wahyudi MD, Syahrina F, Carabelly AN, Puspitasari D, Wasiaturrahmah Y. Formulasi dan stabilitas fisik gel ekstrak batang pisang mauli (Musa acuminata). Dentin J Kedokt Gigi. 2022; 4(3): 161–5. web: https://ppjp.ulm.ac.id/journals/index.php/dnt/article/view/6824
Boteon AP, Kato MT, Buzalaf MAR, Prakki A, Wang L, Rios D, Honório HM. Effect of proanthocyanidin-enriched extracts on the inhibition of wear and degradation of dentin demineralized organic matrix. Arch Oral Biol. 2017; 84: 118–24. doi: https://doi.org/10.1016/j.archoralbio.2017.09.027
Aditya G, Nuraini H, Sekarini WA. Pengaruh material konservasi kolostrum terhadap pelepasan ion Ni. J Teknosains. 2019; 9(1): 12–20. doi:10.22146/teknosains.37727
Liu R-R, Fang M, Zhang L, Tang C-F, Dou Q, Chen J-H. Anti-proteolytic capacity and bonding durability of proanthocyanidin-biomodified demineralized dentin matrix. Int J Oral Sci. 2014; 6(3): 168–74. doi: https://doi.org/10.1038/ijos.2014.22
Amin R, Awad S, Abd El Sattar E. Evaluation of remineralization of grape seed extract versus sodium fluoride on demineralized primary anterior teeth: An in-vitro study. Egypt Dent J. 2019; 65(3): 1977–84. doi: https://doi.org/10.21608/edj.2015.71714
Shahmoradi M, Bertassoni LE, Elfallah HM, Swain M. Fundamental structure and properties of enamel, dentin and cementum. In: Ben-Nissan B, editor. Advances in calcium phosphate bomaterials. Berlin, Heidelberg: Springer; 2014. p. 511–47. doi: https://doi.org/10.1007/978-3-642-53980-0_17
Normayanti N, Soetojo A, Pribadi N. The difference between residual monomer dentin bonding HEMA and UDMA with acetone and ethanol solvent after binding to type I collagen. Dent J. 2018; 51(4): 169–72. doi: https://doi.org/10.20473/j.djmkg.v51.i4.p169-172
Farzad P, Lundgren T, Al-Asfour A, Andersson L, Dahlin C. Integration of dental implants in conjunction with EDTA-conditioned dentin grafts: An experimental study. Dent J. 2021; 9(6): 63. doi: https://doi.org/10.3390/dj9060063
Irianti AN, Kuswandari S, Santoso AS. Effect of formula milk on the roughness and hardness of tooth enamel. Dent J. 2021; 54(2): 78–81. doi: https://doi.org/10.20473/j.djmkg.v54.i2.p78-81
Apriasari ML, Pramitha SR, Puspitasari D, Ernawati DS. Expression of fibroblast growth factor-β and transforming growth factor-β in mauli banana stem (Musa Acuminate) extract gel - treated traumatic ulcer. Trop J Pharm Res. 2021; 18(3): 527–31. doi: https://doi.org/10.4314/tjpr.v18i3.12
Epasinghe DJ, Kwan S, Chu D, Lei MM, Burrow MF, Yiu CKY. Synergistic effects of proanthocyanidin, tri-calcium phosphate and fluoride on artificial root caries and dentine collagen. Mater Sci Eng C. 2017; 73: 293–9. doi: https://doi.org/10.1016/j.msec.2016.11.078
Jowkar Z, Firouzmandi M, Tabibi S. The effect of proanthocyanidin and casein phosphopeptide‐amorphous calcium phosphate on the bond strength durability to caries‐affected dentin. Clin Exp Dent Res. 2021; 7(3): 338–43. doi: https://doi.org/10.1002/cre2.368
Bueno T, da Silva T-A-P, Rizzante F-A-P, Magalhães A-C, Rios D, Honório H-M. Evaluation of Proanthocyanidin-based dentifrices on dentin-wear after erosion and dental abrasion - In situ study. J Clin Exp Dent. 2022; 14(4): e366–70. doi: https://doi.org/10.4317/jced.59071
Prasetyo AI, Kunarti S, Soetojo A, Prasetyo EA. Chemical bond strength difference between 4-meta bonding agents with ethanol and acetone solvent on type I Collagen. J Int Dent Med Res. 2018; 11(2): 567–71. pdf: http://www.jidmr.com/journal/wp-content/uploads/2018/09/34D17_519-Layout.pdf
Zubaidah N, Widjiastuti I, Pribadi N, Putri FK, Muljadi RP, Charyadie FL, Khotimah K. The expression of tumor necrosis factor alpha and alkaline phosphatase due to induction of combination calcium hydroxides and propolis. J Int Dent Med Res. 2021; 14(2): 543–8. pdf: http://www.jidmr.com/journal/wp-content/uploads/2021/07/15-D20_1355_Nanik_Zubaidah_Indonesia.pdf
Copyright (c) 2024 Dental Journal
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
- Every manuscript submitted to must observe the policy and terms set by the Dental Journal (Majalah Kedokteran Gigi).
- Publication rights to manuscript content published by the Dental Journal (Majalah Kedokteran Gigi) is owned by the journal with the consent and approval of the author(s) concerned.
- Full texts of electronically published manuscripts can be accessed free of charge and used according to the license shown below.
- The Dental Journal (Majalah Kedokteran Gigi) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License
