Comparative Pathologic, Immunohistochemical, Ultrastructural and Molecular study of Bovine Papilloma Virus type 1 E5 Oncogene infection in Exotic and Indigenous cattle breeds
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Bovine papillomavirus (BPV) induces benign tumors of cutaneous or mucosa epithelia, called papillomas or warts in cattle and generally regress without eliciting any serious clinical problems in the host, but occasionally persist and provide the focus for malignant transformation to squamous cell carcinoma. This has a negative implication in beef and hide industry. There is paucity of information on the comparative pathology and molecular detection of BPVs in different breeds of nomadic cattle. Consequently, 340 nomadic cattle grazing in Plateau state Nigeria were screened clinically for papillomatosis followed by histopathology. Lesion consistent with papillomatosis were further investigated using immunohistochemistry (IHC), Polymerase chain reaction (PCR) and electron microscopy. Twenty cattle (0.06%) of Friesian, Zebu, Muturu and White Fulani breeds had cutaneous papilloma and skin lesions of the head, neck, shoulders, legs, dorsum, lower abdomen and scrotum. However, only 16 (0.05%) cattle skin biopsy samples were consistent with papillomatosis histopathologically revealing varying degrees of hyperplastic epidermis with acanthosis and orthokeratotic hyperkeratosis. Nuclei in the granular layer of the epidermis were IHC positive for Bovine papillomavirus type I antigen while skin biopsy was BPV-1 E5 oncoprotein gene positive by PCR. Electron microscopy revealed ultrastructural changes consistent with Bovine papillomavirus infection. The distribution and severity of lesion varied in different breeds of nomadic cattle. The detection, diagnosis and characterization of papillomavirus in these cattle enabled the development of autogenous vaccine to immunized cattle. This study highlighted the comparative pathology and molecular characterization of BPVs in different breeds of nomadic cattle, which hitherto was lacking.
Akanbi, O.B., K. Franzke, Adedeji, A.J., Ulrich, R. and Teifke, J.P., 2020. Peste des petits ruminants virus and goatpox virus co-infection in goats. Veterinary Pathology, 57(4), 550-553.
Bauermann, F.V., Joshi, L.R., Mohr, K.A., Kutish, G.F., Meier, P., Chase, C., Christopher-Hennings, J. and Diel, D.G., 2017. A novel bovine papillomavirus type in the genus Dyokappapapillomavirus. Archives of virology, 162(10), 3225-3228.
Betiol, J.C., Kignel, S., Tristí£o, W., Arruda, A.C., Santos, S.K.S., Barbieri, R. and Bettini, J.D.S.R., 2012. HPV 18 prevalence in oral mucosa diagnosed with verrucous leukoplakia: cytological and molecular analysis. Journal of clinical pathology, 65(8), 769-770.
Borzacchiello, G. and Roperto, F., 2008. Bovine papillomaviruses, papillomas and cancer in cattle. Veterinary research, 39(5), p.1.
Carvalho, C.C.R., Batista, M.V.A., Silva, M.A.R., Balbino, V.Q. and Freitas, A.C., 2012. Detection of bovine papillomavirus types, co"infection and a putative new BPV11 subtype in cattle. Transboundary and emerging diseases, 59(5), 441-447.
Cheville, N.F., 2014. Papillomatosis Then and Now. Veterinary Pathology, 51(6), 1049-1050.
Corteggio, A., Altamura, G., Roperto, F. and Borzacchiello, G., 2013. Bovine papillomavirus E5 and E7 oncoproteins in naturally occurring tumors: are two better than one?. Infectious agents and cancer, 8(1), 1-9.
da Silva, F.R., Daudt, C., Cibulski, S.P., Weber, M.N., Varela, A.P.M., Mayer, F.Q., Roehe, P.M. and Canal, C.W., 2017. Genome characterization of a bovine papillomavirus type 5 from cattle in the Amazon region, Brazil. Virus genes, 53(1), pp.130-133.
Daudt, C., Da Silva, F.R.C., Lunardi, M., Alves, C.B.D.T., Weber, M.N., Cibulski, S.P., Alfieri, A.F., Alfieri, A.A. and Canal, C.W., 2018. Papillomaviruses in ruminants: an update. Transboundary and emerging diseases, 65(5), pp.1381-1395.
Elzein, E.T.E., Sundberg, J.P., Housawi, F.M., Gameel, A.A., Ramadan, R.O. and Hassanein, M.M., 1991. Genital bovine papillomavirus infection in Saudi Arabia. Journal of Veterinary Diagnostic Investigation, 3(1), pp.36-38.
Fagbamila, I.O., Meseko, C.A., Adedeji, J.A., Ngulukun, S.S., Akalusi, Y., Dalis, J.S., Akanbi, B.O., Zwandor, N.J., Okpara, J., Ankeli, P.I. and Asala, O.O., 2010. Fusarium graminearum in a Papilloma Virus Infected Friesian Bull in Vom, Nigeria: Case Report. Nigerian Veterinary Journal, 31(4), 302-305.
Olusegun, F., Olalekan, J. and Omotola, B., 2018. Molecular Characterization of Bovine Papillomaviruses Obtained From Cattle Cutaneous Warts in Nigeria. Alexandria Journal for Veterinary Sciences, 59(1), 19-24.
Hatama, S., Nobumoto, K. and Kanno, T., 2008. Genomic and phylogenetic analysis of two novel bovine papillomaviruses, BPV-9 and BPV-10. Journal of General Virology, 89(1), pp.158-163.
Jarrett, W.H., 1985. The natural history of bovine papillomavirus infections. Advances in Viral Oncology, 5, pp.83-101.
Jelinek, F. and Tachezy, R., 2005. Cutaneous papillomatosis in cattle. Journal of Comparative Pathology, 132(1), 70-81.
Jeremiah, O.T., Fagbohun, O.A. and Babalola, O.J., 2016. Molecular detection of bovine papilloma viruses associated with cutaneous warts in some breeds of Nigerian Cattle. International Journal of Biotechnology and Biochemistry, 12(2), 123-130.
Leto, M.D.G.P., Santos Júnior, G.F.D., Porro, A.M. and Tomimori, J., 2011. Human papillomavirus infection: etiopathogenesis, molecular biology and clinical manifestations. Anais brasileiros de dermatologia, 86(2), 306-317.
Lunardi, M., Alfieri, A.A., Otonel, R.A.A., de Alcí¢ntara, B.K., Rodrigues, W.B., de Miranda, A.B. and Alfieri, A.F., 2013a. Genetic characterization of a novel bovine papillomavirus member of the Deltapapillomavirus genus. Veterinary Microbiology, 162(1), 207-213.
Lunardi, M., de Alcí¢ntara, B.K., Otonel, R.A.A., Rodrigues, W.B., Alfieri, A.F. and Alfieri, A.A., 2013b. Bovine papillomavirus type 13 DNA in equine sarcoids. Journal of clinical microbiology, 51(7), 2167-2171.
Meseko CA., 2010. Emergence of severe bovine papilloma virus in cattle with genetic relationship to human papilloma virus. In: 2nd Unibadan Conference of Biomedical Research; July 2010; Ibadan, Oyo State. Accessed 2021 Jan 6.
Munday JS, Thomson N, Dunowska M, Knight CG, Laurie RE, Hills S. Genomic characterisation of the feline sarcoid-associated papillomavirus and proposed classification as Bos taurus papillomavirus type 14. Vet Microbiol. 2015;177(3-4):289–295.
Nascimento, G.A., Souza, E.V., Campos-Ferreira, D.S., Arruda, M.S., Castelletti, C.H., Wanderley, M.S., Ekert, M.H., Bruneska, D. and Lima-Filho, J.L., 2012. Electrochemical DNA biosensor for bovine papillomavirus detection using polymeric film on screen-printed electrode. Biosensors and Bioelectronics, 38(1), 61-66.
Nasir, L. and M.S. Campo, 2008. Bovine papillomaviruses: their role in the aetiology of cutaneous tumors of bovids and equids. Veterinary dermatology, 19(5), 243-254.
Nasir, L. and Reid, S.W.J., 1999. Bovine papillomaviral gene expression in equine sarcoid tumours. Virus research, 61(2), 171-175.
Teifke, J.P., Hardt, M. and Weiss, E., 1994. Detection of bovine papillomavirus DNA in formalin-fixed and paraffin-embedded equine sarcoids by polymerse chain reaction and non-radioactive in situ hybridization. European journal of veterinary pathology: official journal of the European Society of Veterinary Pathology, 1, 5-10.
Teifke, J.P., Löhr, C.V. and Shirasawa, H., 1998. Detection of canine oral papillomavirus-DNA in canine oral squamous cell carcinomas and p53 overexpressing skin papillomas of the dog using the polymerase chain reaction and non-radioactive in situ hybridization. Veterinary Microbiology, 60(2-4), 119-130.
Uzoukwu M., 1979. Some Skin Tumours of Domestic Animals. Nigeria Vet J., 8(1):7-11.
Valarcher, J.F., Leforban, Y., Rweyemamu, M., Roeder, P.L., Gerbier, G., Mackay, D.K.J., Sumption, K.J., Paton, D.J. and Knowles, N.J., 2008. Incursions of foot"and"mouth disease virus into Europe between 1985 and 2006. Transboundary and emerging diseases, 55(1), 14-34.
Zwandor, N.J., Akalusi, Y., Ngulukun, S., Meseko, C., Akanbi, O., Adedeji, A. and Fagbamila, I., 2008. Severe cutaneous bovine papillomatosis in a cattle herd.
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