The Effect of β-Carotene Supplementation on Triglyceride Levels Study on Type 2 Diabetes Mellitus Wistar Rats Fed High-Fat Diet and Induced Streptozotocin-Nicotinamide
Background: Blood triglyceride levels are associated with decreasing insulin sensitivity and increasing insulin resistance in type 2 diabetes mellitus. β-carotene has cytoprotective effect that can improve and protect β-cells of pancreas, resulting in increased insulin synthesis and secretion. β-carotene as antioxidants has important roles as glycemic and lipid control.
Objectives: This research aimed to investigate the effect of β-carotene supplementation on triglyceride levels at high fat diet and streptozotocin-nicotinamide induced of Type 2 Diabetes Mellitus Wistar Rats.
Methods: Eighteen Wistar rats were randomly divided into 3 groups: X1 (negative controls), X2 (positive controls, STZ-NA induced) and X3 (STZ-NA+β-carotene 10 mg/KgBW). High fat diet intervention was given 14 days before diabetic induced in X2 and X3 groups, at the same time the negative control group X1 was given standard feed as a control diet. To condition Wistar rats to be type 2 Diabetes Mellitus, rats in groups X2 and X3 received intraperitonial injections of Streptozotocin 45 mg/KgBW and nicotinamide 110 mg/kgBW on 22nd days after high fat diets intervention. Supplementation of β-carotene was given by nasogastric feeding tube method on every 2 days within 30 days. Plasma Triglyceride levels were measured by GPO-PAP method. The effect of triglyceride levels after β-carotene supplementation between pre and post-test in groups was tested with statistical test using paired t-test, whereas to distinguish the effect of triglyceride levels after β-carotene supplementation between groups was tested with statistical test using one-way anova method.
Results: There was significant effect of triglyceride levels (p=0.000) after β-carotene 10mg/kgBW supplementation on alternate days within 30 days by feeding tube method. Serum triglyceride levels decreased by a mean of 21.90% or 28.42 mg/dl.
Conclusions: This research revealed that supplementation of β-carotene 10 mg/kgBW every 2 days within 30 days by nasogastric feeding tube method can lower triglyceride levels in diabetic Wistar rats.
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