ANTI-DENGUE TYPE 2 VIRUS ACTIVITIES OF ZINC (II) COMPLEX COMPOUNDS WITH 2-(2,4 -DIHYDROXYPHENYL)-3,5,7-TRIHYDROXYCROMEN-4-ONE LIGANDS IN VERO CELLS
Downloads
Dengue virus (DENV) is a disease that is transmitted through Aedes aegypti and Aedes albopictus mosquitoes, and is spread in tropical and sub-tropical regions. Now, dengue or antiviral vaccines for humans do not yet exist, but there are great efforts to achieve this goal. Complex compounds are reported to fungicidal, bactericidal and antiviral activity. Antiviral activity against DENV is an important alternative to the characterization and development of drugs candidate. The purpose of this study was to study zinc(II) compounds with 2-(2,4-dihydroxyphenyl)-3,5,7-trihydroxycromen-4-one ligand on DENV-2 replication in Vero cells. Vero cell lines (African green monkey kidney) was used in this study, maintained and propagated in Minimum Essential Eagle Medium containing 10% fetal bovine serum at 37°C in 5% CO2. The activity of dengue virus was carried out by enzyme-immunosorbent assay (ELISA) method and CellTiter96® Non-Radioactive Proliferation. The value of activity inhibition (IC50) of complex compounds with variations of mol metal: ligand 1:2, 1:3, and 1:4 against dengue virus type 2 (DENV2) was 2.44 μg/ml, 2.75 μg/ml, respectively and 2.00 μg/ml, also the toxicity value (CC50) of complex compounds with variation mol metal: ligand 1:4 for Vero cells is 3.59 μg/ml. The results of this study were indicate that these properties have been shown to inhibit anti-dengue type 2 virus (DENV-2), but are also toxic in Vero cells. Including previous study about complex compound interaction with dengue virus type 2 activity, Zn(II) more reactive compound then Cu(II), and Co(II). The comparison with Cu(II) complex compound, it has been revealed that Co(II) and Zn(II) is more toxic, was found to be nontoxic to human erythrocyte cells even at a concentration of 500 μg/ml.
Carrington LB, Simmons CP, 2014. Human to mosquito transmission of dengue viruses. Front. Immunol. 5(290): 1-8
Pongsiri A, Ponlawat A, Thaisomboonsuk B, Jarman RG, Scott TW, Lambrechts L, 2014. Differential susceptibility of two field Aedes aegypti populations to a low infectious dose of dengue virus. Plos One. 9(3): 1-6
Paixí£o ES, Teixeira MG, Rodrigues LC, 2017. Zika, chikungunya and dengue: the causes and threats of new and re-emerging arboviral diseases. BMJ Glob. Health. 3: 1-6
Gurugama P, Garg P, Perera J, Wijewickrama A, Seneviratne SL, 2010. Dengue viral infections. Indian J. Dermatol., 55(1): 68-78
Oliveira AFCS, Teixeira RR, Oliveira AS, Souzza APM, Silva ML, Paula SO, 2017. Potential Antivirals: Natural Products Targeting Replication Enzymes of Dengue and Chikungunya Viruses. Molecules, 22(505): 1-20
Ho MR, Tsai TT, Chen CL, Jhan MK, Tsai CC, Lee YC, Chen CH, Lin CF, 2017. Blockade of dengue virus infection and viral cytotoxicity in neuronal cells in vitro and in vivo by targeting endocytic pathways. Sci. Rep. 7(6910): 1-11
Sun P, Kochel TJ, 2013. The battle between infection and host immune responses of dengue virus and its implication in dengue disease pathogenesis. Sci. World J. 2013: 1-11
Shafee N, AbuBakar S, 2011. Characterization of dengue type 2 NGC virus infection in C6/36, Vero and MRC-5 cells. Intl. J. Virol., 7(1): 24-32
Gammoh NZ, Rink L, 2017. Zinc in infection and inflammation. Nutrients, 9(624): 1-25
Lee SY, Ching YW, Shafee N, 2017. Zinc induces normoxic accumulation of transcriptionally active hypoxia-inducible factor
-Alpha in mammary epithelial cells. Molecular Biology, 51(1): 89-95
Sucipto TH, Churrotin S, Setyawati H, Martak, F, Mulyatno KC, Amarullah IH, Kotaki T, Kameoka M, Yotopranoto S, Soegijanto S, 2018. A new copper(II)-imidazole derivative effectively inhibits replication of DENV-2 in Vero cell. Afr. J. Infect. Dis., 12(S): 116-119
Sucipto TH, Churrotin S, Setyawti H, Mulyatno KC, Amarullah IH, Ueda S, Kotaki T, Sumarsih S, Wardhani P, Bendryman SS, Aryati, Soegijanto S, Kameoka M, 2017. Inhibitory activity of cobalt(II)-morin complex against the replication of dengue virus type 2. Indones J. Trop. Infect. Dis. 6(6): 141-144
Ammerman NC, Beier-Sexton M, Azad AF, 2009. Growth and maintenance of vero cell lines. Curr. Protoc. Microbiol. 11(1): 1-10
Plotkin BJ, Sigar IM, Swartzendruber JA, Kaminski A, 2018. Anaerobic growth and maintenance of mammalian cell lines. J. Vis. Exp. 137: 1-6
Koishi AC, Zanello PR, Bianco EM, Bordingon J, dos Santos CND, 2012. Screening of dengue virus antiviral activity of marine seaweeds by an in situ enzyme-linked immunosorbent assay. Plos One. 7(12): 1-11
Kirubavathy SJ, Velmurugan R, Parameswari K, Chitra S, 2014. Synthesis, characterization, single crystal XRD, in vitro antimicrobial and cytotoxicity study of tris(ethylenediamine)cobalt(III)chloride oxalate trihydrate. Arab. J. Chem. xxx: 1-6
Inba PJK, Annarej B, Thalamuthu S, Neelakantan MA, 2013. Cu(II), Ni(II), and Zn(II) complexes of salan-type ligand containing ester groups: synthesis, characterization, electrochemical properties, and in vitro biological activities. Bioinorg. Chem. Appl. 2013: 1-12
Sucipto TH, Churrotin S, Setyawati H, Kotaki T, Martak, F, Soegijanto S, 2017. Antiviral activity of copper(II)chloride dihydrate against dengue virus type-2 in Vero cell. Indones J. Trop. Infect. Dis. 6(4): 84-87
Liu Y., Guo M., 2015, Studies on transition metal-quercetin complexes using electrospray ionization tandem mass spectrometry, Molecules, No. 20, 8583-8594
Ejelonu BC, 2016. Potential of copper and its complexes of therapeutic agents. World J. Res. Rev. 3(3): 47-52
Rosu T, Pahontu E, Illies DC, Georgescu R, Mocanu M, Leabu M, Shova S, Gulea A, 2012. Synthesis and characterization of some new complexes of Cu(II), Ni(II), and V(IV) with Schiff base derived from indole-3-carboxaldehyde. Biological activity prokaryote and eukaryotes. Eur. J. Med. Chem. 53: 380-389
Copyright (c) 2019 Indonesian Journal of Tropical and Infectious Disease
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The Indonesian Journal of Tropical and Infectious Disease (IJTID) is a scientific peer-reviewed journal freely available to be accessed, downloaded, and used for research. All articles published in the IJTID are licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which is under the following terms:
Attribution ” You must give appropriate credit, link to the license, and indicate if changes were made. You may do so reasonably, but not in any way that suggests the licensor endorses you or your use.
NonCommercial ” You may not use the material for commercial purposes.
ShareAlike ” If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
No additional restrictions ” You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
