Soegeng Soegijanto, Desiana Wikanesthi, Eva Chilvia, Oedojo Soedirham

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Since January 2014, Soerya Hospital has found many cases with positive result of NS or IgM and IgG Dengue. The clinical manifestations mostly were high fever with headache, vomiting and also malaise convulsion and unconsciousness. Aim of the study is to find out an appropriate diagnosis of Dengue Virus Infection. Observasional study had been done since January–April 2014 with 50 cases of dengue Virus Infection. The diagnostic procedure was made based on the WHO 2011 criteria. Result Many cases had come with fever within couple days, some of them showed convulsions. Therefore, it should be made a differential diagnosis with other disease, such as acute tonsilopharingitis, etc. The patient also had to be tested with NS1 if the patient come in the first, second and third day of fever and followed by IgM/IgG dengue on the fourth, fifth or sixth days of fever. The diagnosis of Dengue Virus Infection was made based on the WHO criteria 2011. This study showed that not all cases showed positive result of NS1 or IgM/IgG dengue on the first or second test. For the negative result, we should not think that the case is not a case of Dengue Virus Infection, especially if it happens at Aedes aegypti breeding season, the patient should be observed and performed the test again to get a proper diagnosis for Dengue Virus Infection. Monitoring clinical manifestation should always be done, to predict the appropriate diagnosis of Dengue Virus Infection.


Dengue Virus; Diagnosis Dengue Virus NS1; IgM test; IgG test; WHO criteria

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World Health Organization. Geneva, Switzerland: WHO; 2010. Working to Overcome the Global Impact of Neglected Tropical Diseases. First WHO report on Neglected Tropical Diseases.

Bandyopadhyay S, Lum LC, Kroeger A. Classifying dengue: a review of the difficulties in using the WHO case classification for dengue haemorrhagic fever. Trop Med Int Health. 2006; 11: 1238–55. [PubMed].

World Health Organization. Dengue and Dengue Haemorrhagic Fever. Fact sheet 117, 2009 [cited 28 November 2011] Available from:

World Health Organization. Geneva, Switzerland: WHO. 2009. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control.

Avirutnan P, Punyadee N, Noisakran S, Komoltri C, Thiemmeca S, Auethavornanan C, 2006. Vascular Leakage in Severe Dengue Virus Infections: A potential Role for the Nonstructural Viral protein NS1 and Complement. J. of Infect. Dis. 193: 1078–1088.

Darmowandowo W, Faizi M, 2001. Identifikasi Jenis Infeksi Primer dan Sekunder melalui penetapan Rasio IgM & IgG pada Penderita Demam Berdarah Dengue 2001. TDC Unair, h. 12–26.

Departemen Kesehatan Republik Indonesia, 2003. Pencegahan dan Penanggulangan Penyakit Demam Dengue dan Demam Berdarah Dengue. Terjemahan dari WHO Regional Publication SEARO no. 29: Prevention and Control of Dengue Haemorrhagic Fever. Kerja sama WHO dan Dep.Kes. RI, Jakarta.

Dins Kesehatan Propinsi Jawa Timur 2007. Situasi Penyakit DBD Propinsi Jatim & Kebijakan Program P2 DBD. Dinas Kesehatan Propinsi Jawa Timur, Surabaya.

Dussart P, Labeau B, lagathu G, Louis P, Nunes MRT, Rodriques SG, Storck-Hermann C, Cesaire R, Morvan J, Flamand M, Baril L, 2006. Evaluation of an Enzyme Immunoassay for Detection of Dengue Virus NS1 Antigen in Human Serum. Clinical and Vaccine Immunology, 13(11): 1185–1189.

Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, Matheus S, Baril L, 2008. Evaluation of Two New Commercial Tests for the Diagnosis of Acute Dengue Virus Infection Using Ns1 Antigen Detection in Human Serum. Plos Negl. Trop. Dis. 2(8): 57–61.

Faizi M, 1998. Validitas Ratio IgM/IgG sebagai Pembeda Infeksi Primer dan Sekunder pada Penderita Demam Berdarah Dengue. Penelitian Karya Ilmiah Akhir untuk Dokter Spesialis 1 Ilmu Kesehatan Masyarakat Fakultas Kedokteran Universitas Airlangga/RSUD. Dr. Soetomo, Surabaya.

Flamand M, Megret F, Mathieu M, Lepault J, Rey F, Deudel V, 1999. Dengue Virus Type 1 Nonstructural Glycoprotein NS1 is Secreted form Mammalian Cells as a Solube Hexamer in Glycosylation-Dependent Fashion. J of Virol, 73(7): 6104–6110.

Flammand M, Alcon – LePoder, Drouet MT, Sivard P, 2005. Detection of NS1 from Dengue Virus: basis for Early Diagnosis and Aprognostic Marker of Disease Progression (2). Virologi Departement, Pasteur Institute, Paris, France, p. 43.

V.Kumarasamy A.H, Abdul WahapS.K,Chua,Z.Hassan. 2007. Evaluation of a commercial dengue NS1 antigen-capture ELISA for laboratory diagnosis of acute dengue virus infection. Journal of virology Method, Volume 140, Issue 1-2, March 2007, Pages 75–79.

Pei Yun Shu, Cheng-Li Kuang, Chang Fen Shi.2003.Comparison of capture Immunoglobulin M (IgM) and IgG Enzyme-Linked Immunosorbent Assay (ELISA) and Nonstructural Protein NS1 Serotype – Spesific IgG ELISA for differentiation of Primary and Secondary Dengue Virus Infections. Received 13 January 200., Returned for modification 17 March 2003/Accepted 1 April 2003.


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