PRODUCTION AND CHARACTERIZATION OF IMMUNOGLOBULINE YOLK AS ANTI ANTIGEN MEMBRANE TOXOPLASMA GONDII

Heni Puspitasari, Yuliana Praptiwi, Lucia Suwanti

= http://dx.doi.org/10.20473/ijtid.v6i2.1365
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Abstract


Toxoplasma gondii is an obligate parasite intracellular which can infected human and other mammalian. Immunoglobulin Y technology  offers several advantages better than antibody production in mammals. This research was aimed to get immunoglobulin Y from egg yolk, to prove that antibody against membrane T. gondii antigen can produced from immunoglobulin Y and to know the characterization of  immunoglobuline Y according to  molecular weight by SDS PAGE and reactivation of  antibody antigen  by Western Blott. This research devided  from many step : passase tachyzoites T. gondii into mice by peritoneal infection, cultivated  the tachyzoite from intraperitoneal fluid, preparation of  membrane antigen tachyzoite T. gondii, then  immunization laying hens with membrane antigen, extraction and purification immunoglobuline Y from egg yolk and then protein analyzed by SDS PAGE and Western Blott.  The result of this resarch showed that immunoglobulin Y from egg yolk can  produced antibody against protein membrane T. gondii. The result of analyzed profile protein immunoglobuline  Y according SDS PAGE  has molecular weight 179,8 kDa. Analyzed from Western Blott showed that immunoglobulin Y can recognize antigen epitope of  T. gondii on molecular weight 35,7 kDa and 78,8 kDa.


Keywords


Toxoplasma gondii; anti membrane T.gondii; immunoglobulin Y anti membrane

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References


Hanafiah, M., Wisnu N, Mufti K, dan Fadrial K. 2009. Produksi dan Isolasi Protein Membran Stadium Bradizoit Toxoplasma gondii: Suatu Usaha untuk Mendapatkan Material Diagnostik dalam Mendiagnosa Toksoplasmosis. Fakultas Kedokteran Hewan Universitas Syiah Kuala. Aceh. Vol. 10 No. 3: 156–164.

Hiswani. 2003. Tesis: Toxoplasmosis Penyakit Zoonosis Yang Perlu Diwaspadai Oleh Ibu Hamil. Fakultas Kesehatan Masyarakat. Universitas Sumatera Utara. Chandra, G. 2001. Toxoplasma gondii: Aspek Biologi, Epidemiologi, Diagnosis dan penatalaksanaannya.

Chandra, G. 2001. Toxoplasma gondii: Aspek Biologi, Epidemiologi, Diagnosis dan penatalaksanaannya. http://www.emedice.com. (Juni 2011).

Ardhiani, F. 2008. Insidensi Toxoplasmosis pada Babi di RPH Pengirian Surabaya dan RPH Gadang Malang. Fakultas Kedokteran Hewan Universitas Airlangga. Surabaya.

Arabpour, M., Mojgan B. Maryam N., Seyyed H.A. 2011. African Journal of Biotechnology Vol. 10(40): Cloning and expression of Toxoplasma gondii tachyzoite P22 protein. Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran., pp. 7746–7750.

Suwanti, L.T. 1996. Identifikasi dan produksi Antibodi Monoklonal Protein Membran Toxoplasma gondii Stadium takizoit. Tesis Pascasarjana Universitas Gadjah Mada. Yogyakarta.

Chalghoumi, R., B. Yves, P. Daniel and T. Andre. 2009. Hen Egg Yolk Antibodies (IgY), Production and Use for Passive Immunization Against Bacterial Enteric Infection in Chicken. Gembloux Agriculture University. Belgium. 295–308.

Michael, A., S. Meenatchisundaram, G. Parameswari, T. Subbraj, R. Selvakumaran and S. Ramalingam. 2010. Chicken Egg Yolk Antibodies (IgY) as an Alternative to Mammalian Antibodies. Indian J. Science Technology. 3(4): 468–474.

Ko K. and Ahn D.U, 2007. Preparation of Immunoglobulin Y from Egg Yolk Using Ammonium Sulfate Precipitation and Ion Exchange Chromatography. Poultry Science. 86: 400–407.

He, Y and Pamela J.B. 2011. Strukture of FcRY, an avian immunoglobuli receptor related to mammalian mannose receptor, and its compleks with IgG. California Institute of Technology. USA. Page: 12431–12436.

Abbas, A.K., A.H. Lichtman and J.S. Pober. 2000. Cellular and Molecular Immunology. W.B. Saunders Company, Philadelphia. pp. 235–338.

Carruthers, V.B. 2002. Host Cell Invasion by the Opportunistic Pathogen Toxoplasma gondii. Acta Trop. 81: 111–122.


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