Moringa oleifera Leaf Ethanol Extract Inhibits Toxoplasma gondii Tachyzoites Replication
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The various infection routes of Toxoplasma gondii that are close to daily life strongly support the incidence of toxoplasmosis. The emergence of drug-resistant Toxoplasma gondii strains raises future concerns. Moringa leaf ethanol extract has been shown to have several anti-pathogen activities, which could have an anti-Toxoplasma effect. This research was conducted to analyze the anti-Toxoplasma effect of moringa leaf ethanol extract against tachyzoites replication in Toxoplasma gondii and the correlation between extract doses with the number of tachyzoites. Mice were divided into five groups. The negative control group (Group I) received CMC-Na solution. The positive control group (Group II) received spiramycin 100 mg/kg BW. The treatment groups received moringa leaf ethanol extract 250 mg/kg BW (group III), 500 mg/kg BW (group IV), and 1000 mg/kg BW (group V), respectively. Mice were injected with 1 x 105 tachyzoites/0.1 mL/mice intraperitoneally on the first day. Moringa leaf ethanol extract and spiramycin were given orally once daily for three days. The number of tachyzoites in the intraperitoneal fluid was calculated on the fifth day. The results have shown that there were significantly lower differences (P < 0.05) in group IV (P = 0.021) and group V (P = 0.022) compared to group I. There was also a significant negative correlation between the extract doses and the number of tachyzoites (P = 0.000; r = -0.781). Moringa oleifera leaf ethanol extract has an anti-Toxoplasma effect by inhibiting the tachyzoite replication at 500 mg/kg BW and 1000 mg/kg BW.
Flegr J, Prandota J, SoviÄková M, Israili ZH. Toxoplasmosis - a Global Threat. Correlation of Latent Toxoplasmosis with Specific Disease Burden in a Set of 88 Countries. PLOS ONE. 2014;9(3):e90203.
Polanunu NFA, Wahyuni S, & Hamid F. Seroprevalence and associated risk factors of Toxoplasma gondii infection among pregnant mother in Makassar, Indonesia. PLOS ONE. 2021;16(6):e0245572.
Wang D, Liu H, Ma X, Ma Y, Li Y, Yang B, et al. Toxoplasma gondii Infection in Immunocompromised Patients: A Systematic Review and Meta-Analysis. Front Microbiol. 2017;8:389.
Centers for Disease Control and Prevention (CDC). Epidemiology & Risk Factors of Parasites - Toxoplasmosis. Global Health, Division of Parasitic Diseases and Malaria. 2018.
Montazeri M, Mehrzadi S, Sharif M, Sarvi S, Tanzifi A, Aghayan SA, et al. Drug Resistance in Toxoplasma gondii. Front Microbiol. 2018;9(2857):1-12.
Nishi L, Sanfelice RADS, da Silva Bortoleti BT, Tomiotto-Pellissier F, Silva TF, Evangelista FF, et al. Moringa oleifera Extract Promotes Apoptosis-like Death in Toxoplasma gondii Tachyzoites in Vitro. Parasitol. 2021;148(12):1447-1457.
Mittal A, Sharma M, David A, Vishwakarma P, Saini M, Goel M, et al. An Experimental Study to Evaluate the Anti-Inflammatory Effect of Moringa oleifera Leaves in Animal Models. Int J of Basic Clin Pharmacol. 2017;6(2):452-457.
Ahmadu T, Ahmad K, Ismail SI, Rashed O, Asib N, Omar D. Antifungal Efficacy of Moringa oleifera Leaf and Seed Extracts against Botrytis cinerea causing Gray Mold Disease of Tomato (Solanum lycopersicum L.). Braz J Biol. 2021;81(4):1007-1022.
Fouad EA, Abu Elnaga ASM, Kandil MM. Antibacterial Efficacy of Moringa oleifera Leaf Extract against Pyogenic Bacteria Isolated from a Dromedary Camel (Camelus dromedarius) Abscess. Vet World. 2019;12(6):802-808.
Yasi R, Restiani SH. The Larvicidal Activity of Moringa oleifera Extract Leaf to The Larva's Aedes aegypti Mortality. J Agromedicine Med Sci. 2018;4(3):159-164.
Kashyap P, Kumar S, Riar CS, Jindal N, Baniwal P, Guiné RPF, et al. Recent Advances in Drumstick (Moringa oleifera) Leaves Bioactive Compounds: Composition, Health Benefits, Bioaccessibility, and Dietary Applications. Antioxidants. 2022;11(2):402.
Putra IWDP, Dharmayudha AAGO, Sudimartini LM. Identification of Chemical Compounds Ethanol Extract Leaf Moringa (Moringa oleifera L.) in Bali. Indonesia Medicus Veterinus. 2016;5(5):464-473.
Syahputra RA, Sutiana A, Silitonga PM, Rani Z, Kudadiri A. Extraction and Phytochemical Screening of Ethanol Extract and Simplicia of Moringa Leaf (Moringa oleifera Lam.) From Sidikalang, North Sumatera. Int J Sci Technol Manag. 2021;2(6):2072-2076.
Rani NZA, Husain K, Kumolosasi E. Moringa Genus: A Review of Phytochemistry and Pharmacology. Front Pharmacol. 2018;9:108.
Kadhim EJ, AL-Shammaa DA. Phytochemical Characterization using GC-MS Analysis of Methanolic Extract of Moringa oleifera (Family Moringaceae) Plant Cultivated in Iraq. J Chem Mater Res. 2014;6(5)
Wink M. Medicinal Plants: a Source of Anti-Parasitic Secondary Metabolites. Molecules. 2012;17(11):12771-12791.
Tedyanto CP, Wihanto L, Hendrata AP. Hepatoprotective Effect of Dried Red Jujube Fruit Extract Against Acetaminophen-Induced Acute Hepatotoxicity. Cureus. 2023;15(1):e33272.
Nishu, Jee C. Preliminary Phytochemical Screening and Thin Layer Chromatography of Selected Extract of Moringa oleifera Leaf. Int J Chem Stud. 2020; 8(5):2407-2409.
Nguyen TT, Kamyingkird K, Phimpraphai W, Inpankaew T. Viability of Toxoplasma gondii Tachyzoites in Different Conditions for Parasite Transportation. Vet World. 2022;15(1):198-204.
Omar M, Abaza BE, Mousa E, Ibrahim SM, Rashed HE, Farag TI. Effect of Spiramycin versus Aminoguanidine and Their Combined Use in Experimental Toxoplasmosis. J Parasit Dis. 2021;45: 1014-1025.
Jesus AD, Pusec CM, Nguyen T, Keyhani-Nejad F, Gao P, Weinberg SE, et al. Optimized Protocol to Isolate Primary Mouse Peritoneal Macrophage Metabolites. STAR Protoc. 2022;3(4):101668.
Mehganathan P, Rosli NA. A Review on Extraction of Bioactive Compounds from Moringa oleifera Leaves: Their Principle, Advantages, and Disadvantages. Med Aromat Plants. 2022;11(1):430.
Naumov AV, Wang C, Chaput D, Ting L, Alvarez CA, Keller T, et al. Restriction Checkpoint Controls Bradyzoite Development in Toxoplasma gondii. ASM. 2022;10(3):e00702-22.
McCharthy JS, Wortmann GW, Kirchhoff LV. Drugs for Protozoal Infections Other Than Malaria. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases, 8th ed. Elsevier. 2014;41:510-518.
Shrivastava M, Prasad A, Kumar D. Evaluation of Anti-Malarial Effect Moringa oleifera (Lam) in Plasmodium yoelii Infected Mice. Indian J Pharm Sci. 2021;83(6):1221-1228.
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