EVALUATION OF ANTI PCL-1 ANTIBODY TITER IN A GROUP OF HEALTHY SCHOOL CHILDREN WHO LIVE IN LEPROSY ENDEMIC AREA FROM 2007–2010
The “Iceberg phenomene” has been used to explain this situation which indicate that these new leprosy cases is originated from Subclinical Leprosy. Fifty eight healthy school children who live in Jeneponto Regency, a leprosy endemic area in South Sulawesi were recruited. The first examination was performed in 2007 and sera samples were kept in deep freeze refrigerator. In 2010 these children were re-examined for clinical leprosy and sera were collected again. ELISA study was performed simultaneously to these 58 pairs of sera (2007 & 2010) for measuring the titer of IgM anti PGL-1 antibody (ELISA) and the level 605u/ml was regarded as cut off value. After three years evaluation, none of these children showed any clinical signs of leprosy, but 20 of 22 ( 90.9%) children were remained sero-positive and only 2 (9.1%) became sero-negatives. In other sites, 5 children that previously sero-negatives became sero-positives after 3 years. Eight of 10 (80%) children who showed sero (+) with high titer (>1.000u/ml) in 2007, were also remained in high titer. The mean titer of 2007 was 627.8 u/ml, and after3 years became 723.9 u/ml (p<0.05). Although there is no progression from Subclinical to Manifest Leprosy cases among these children, the number of sero (+) cases were increased and the mean titer of IgM anti PGL-1 antibody was significantly increased.. The majority who previously showed high anti PGL-1 antibody titer, remained in high level. This study support the “iceberg phenomene” theory in Leprosy.
Leprosy, 12th THE ICEBERG PHENOMENA IN LEPROSY ed. WHO Technical Report Series. No. 874.
2. WHO (2009). Global Leprosy Situation. Weekly Epidemiological
Record. No. 33. 14 August 2009.August 2009.
3. James WD, Berger TG, Elston DM. (2006). Hansen�s DiseaseHansen�s Disease (Chapter 17) in Andrews� Diseases of the skin. Clinical Dermatology. 10th Edition.
4. Agusni I. (2003). Leprosy. An ancient disease with a lot of mysteries. Inaugural Speech. Airlangga University Press.
5. Moschella SL. (2004). An update on the diagnosis and treatment of
leprosy. Clinical Review. JAAD 2004.
6. Izumi S, Fujimura T, Ikeda M et al. (1990). Novel gelatin particle agglutination test for serodiagnosis of leprosy in field. J Clin
Microbiol 28: 525–29
7. Buchanan TM. (1994). Serology of leprosy. In (Hastings &OpromollaBuchanan TM. (1994). Serology of leprosy. In (Hastings & Opromolla Eds) Leprosy. 2nd Ed. Churchill Livingstone. Edinburg.Churchill Livingstone. Edinburg.
8. Douglas, JT, Celona RV,Douglas, JT, Celona RV, et al. (1987). Serological reactivity and early detection of leprosy among contacts of lepromatous patients in Cebu, the Philippines. Int. J. Lepr. Other Mycobact. Dis. 55: 718–21.Other Mycobact. Dis. 55: 718–21.
9. Anjarwati, DU. (2008). Sero-epidemiological study on leprosy amongAnjarwati, DU. (2008). Sero-epidemiological study on leprosy among school children in Pacitan Regency, East Java. Thesis. Postgraduatein Pacitan Regency, East Java. Thesis. Postgraduate
Program, Airlangga University.
10. Shu, H. (1993). Study on subclinical infection with M.leprae- A follow up. Acta Academiae Med, 15: 17–23.
11. Agusni I, Kardjito T, Putera ST et al. (2001). Subclinical Leprosy in Mandangin island. A cohort study on clinical and laboratory (Part I).
Indonesian Med J 51(6): 198–202.
12. Rimayani S. (2007). Anti PGL-1 antibody profile among schoolRimayani S. (2007). Anti PGL-1 antibody profile among school
children in Jeneponto Regency, South Sulawesi. Thesis. Postgraduate
Program, Hasanuddin University.
13. Sanches Z, Malik AT, Lambert P. (1986). Simplification and
standarization of serodiagnostic tests for leprosy based on phenolic
glycolipid-1 (PGL-1) antigen. Lepr Rev 57: 83–89.
14. Agis F, Schlich P, Cartel JL, et al (1988). Use of anti M.leprae
phenolic glycolipid antibody detection for early diagnosis and
prognosis of leprosy. Int J Lepr 56: 527.
15. Agusni I. (1997). The change of immuno-pathological pattern as an indicator for the management of subclinical leprosy. Disertation.
Postgraduate Program, Airlangga University.
16. Agusni I, Kardjito T, Soedewo FH et al. (2001). Subclinical Leprosy in Mandangin island, Madura (part II). A preliminary study of
serial surveys in leprosy endemic area. Indonesian Med J 51(12):
17. Godal T, Nagassi K. (1973). Subclinical infection in leprosy. Br Med J 3: 557–9.
18. Bharadwaj V. (1982). A preliminary report on subclinical infection
in leprosy. Lepr India 54: 220–227.
19. Adriaty D, Agusni I, Izumi S et al. (2008). The level of leprosy
sero-positive cases among school children in northern coastal areas
of East Java Province. 12th National Congress of Indonesian Society
of Dermato-venereology. PERDOSKI Palembang,
20. Erliyati. (2008). Subclinical leprosy among traditional and modern
moslem community at Pragaan Subdistric, Pamekasan, Madura.
Thesis. Tropical Medicine Postgraduate Program, Airlangga
21. Kuswiyanto. (2009). Sero-epidemiological study in leprosy among
school children who live in dry and wet environment area. Thesis.
Microbiology Postgraduate Program. Airlangga University.
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