OVERVIEW OF NUCLEAR FACTOR-KB (NF-KB) AND NON-STRUCTURAL PROTEIN 1 (NS1) IN PATIENTS WITH DENGUE FEVER IN PREMIER HOSPITAL, SURABAYA

Ni Nyoman Budiutari, Yoes Prijatna Dachlan, Jusak Nugraha

= http://dx.doi.org/10.20473/ijtid.v7i5.9955
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Abstract


Dengue fever (DF) is an acute viral fever caused by RNA virus that is transmitted by Aedes aegypti and Aedes albopictus mosquitoes. DF is also called viral arthropod-borne disease and is accompanied by headaches, joint and muscle pain. The main target of dengue infection is macrophages or monocytes and dendritic cells (DC). Infected DC is caused the viral replication and the endocytosis into endosomal, easier, thus inducing the activation of NF-ĸB transcription factor to produce proinflammatory cytokines such as Tumor Necrosis Factor-α (TNF-α), Interleukin-1 (IL-1), IL-6, IL-12 and chemokine. NF-kB is one of the transcription factors involved in the regulation of the expression of various cytokines, chemokines and anti/pro-apoptotic proteins during infection and act as indicator of disease severity. Infected DC cells are secreted NS1 protein which is the co-factor needed for viral replication and can be detected in the first eight days. The level will be higher in the initial phase of fever. The purpose of this study was to analyze the description of NF-kB and NS1 levels in the serum of patients with dengue fever through observational analytic studies through a cross-sectional approach. This study was done on 40 patients with dengue fever and 10 healthies people as negative controls. NS1 was analyzed in serum of Panbio rapid test and NF-kB level were measured by sandwich ELISA. The results are showed positive and negative NS1 results in dengue fever patients. The average NF-kB serum level in dengue fever patients was found to be higher than the control. NF-ĸB level in negative NS1 was higher than the NS1 positive group. It is showed that NS1 is detected both in the acute phase. The detection of NF-ĸB is showed the involvement of transcription factors in the development of dengue virus infection and has a protective role for host cells.


Keywords


Dengue Fever, Nuclear Factor-ĸB, NS1 Protein, Viral Infection, Tropical Disease.

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References


WHO. Dengue and Severe Dengue. From World Health Organization. 2017. Available from: http://www.who.int/mediacentre/factsheets/ fs117/en/.

Dow B. Mosquito wars: an overview of dengue fever virus. Modern Biol and Chemic Threats. WordPress. 2017. Philadelphia, PA.

Kementerian Kesehatan RI. Hari demam berdarah dengue: situasi DBD. Infodatin Pusat Data dan Informasi Kementerian Kesehatan RI. 2016. Available from: http://www.depkes.go.id/resources/download/ pusdatin/infodatin/infodatin-demam-berdarah.pdf

Tan TY, Chu JJH. Dengue virus-infected human monocytes trigger late activation of caspase-1, which mediates pro-inflammatory IL-1β secretion and pyroptosis. J Gen Virol. 2013;94(PART10):2215-20.

Aamir M, Masood G, Aamir V, Rasheed A, et al. Gender difference in patients with dengue fever admitted in a teaching hospital Lahore. PJMHS. 2014:08.

Alcala AC, Medina F, Gonzales-Robles A, Salazar-Villatoro L, Fragoso-Roriano RJ, Vásquez C, et al. The dengue virus non-structural protein 1 (NS1) is secreted efficiently from infected mosquito cells. Virology. 2016:488:278-87.

Sun P, Kochel TJ. The battle between infection and host immune responses of dengue virus and its implication in dengue disease pathogenesis. Sci World J. 2013.

Zhao Jun, Minassian A, Shanping He, et al. Recent advances on viral manipulation of NF-ĸB signalling pathway. Curr Opin Virol. 2015:15:103-111.

Silva BM, Sousa LP, Gomes-Ruis AC, Leite G, et al. The dengue

virus nonstructural protein 1 (NS1) increase NF-kB transcriptional activity in hepG2 cells. Arch Virol .2011:156:1275-79.

World Health Organization. Comprehensive guidelines for prevention and control of dengue fever and dengue haemorrhagic fever. WHO. 2011:196 (Cited 15 September 2018). Available from, http://www. searo.who.int/entity/vector_borne_tropical_diseases/documents/ SEAROTPS60/en/.

Soegijanto S. Demam berdarah dengue. Surabaya. Airlangga University Press; 2012:2.

Muller DA, Alexandra CI, Young PR. Clinical and laboratory diagnosis of dengue virus infection. The Infec Diseases J. 2017:215:S89-95.

Kalayanarooj S. Clinical manifestations and management of dengue/ DHF/DSS. Trop Med Health. 2011:39:83-87.

Aryati A, Trimarsanto H, Yohan B, Wardhani P, Fahri S, Sasmono RT. Performance of commercial dengue NS1 ELISA and molecular analysis of NS1 gene of dengue viruses obtained during surveillance in indonesia. BMC Infec Diseases. 2013.

Megariani, Rinang M, et al. Uji diagnostik pemeriksaan antigen nonstruktural 1 untuk deteksi dini infeksi virus dengue pada anak. Padang: Ilmu Kesehatan Anak Universitas Andalas. 2014:6.

Aryati, Rini P. SD dengue duo® (NS1, IgG, IgM) rapid test dalam menunjang diagnosis infeksi virus dengue. Indonesian J Clin Patholog and Med Lab. 2010.

David GN. The relationship of interacting immunological component in dengue pathogenesis. Virol J Review. 2009.

Tambunan BA, Aryati, Husada D. Evaluation of PlateliaTM. Dengue NS1 antigen assay for early diagnosis of acute dengue infection. Presented at 17th European Congress of Clinical Microbiology and Infection Diseases, ICC, Munich, Germany. 2011.

Blacksell SD, Mammen MPJr, Thongpaseuth S, Gibbons RV, Jarman RG, et al. (2008). Evaluation of the panbio dengue virus non-structural 1 antigen detection and immunoglobulin M antibody enzyme-linked immunosorbent assays for the diagnosis of acute dengue infections in Laos. Virolog. p43-49.

Dussart P, Petit L, Labeau B, Bremand L, Leduc A, et al. Evaluation of two new commercial test for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. Plos Negl Trop Dis. 2008.

Murphy K, Weaver C. Janeway’s immunology 9th edition. New York and London. Garland Science. 2017.

Dev A, Iyer S, Razani B, Cheng G. NF-ĸB and innate immunity. Curr Top Microbiol Immunol. 2011.

Lin JC, Shih-Ching L, Cen WY, Yen YT, Lai CW, et al. Dengue viral protease interaction with NF-kB inhibitor α/β result in endothelial cell apoptosis and hemorrhage development. J Immunol. 2014.

Cheng, YL, Lin YS, Chen CL, Wan SW, Ou YD, et al. Dengue virus infection cause the activation of distinct NF-kB pathways for inducible nitric oxide synthase and TNF-α expression in RAW264.7 cells. Mediators Inflamm.. 2015(PART2015):274025.

Aye KS, Charngkaew K, Win N, et al. Pathologic highlights of dengue hemorrhagic fever in 13 autopsy cases from myanmar. Hum Pathol. 2014:45: 1221-33.

Adikari TN, Gomes L, Wickramasinghe N, Salimi M, et al. Dengue NS1 antigen contributes to disease severity by inducing interleukin (IL)-10 by monocytes. Clin Exp Immunol. 2015:1:90-100.


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