Screening of Amylolytic Bacteria from Mina Padi Aquaculture in Panembangan Village, Cilongok District, Banyumas, Central Java
Screening of Amylolytic Bacteria from Mina Padi Aquaculture
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Amylolytic bacteria play an important role in the ecosystem, especially as probiotic and bioremediation agents in cultivation, as examples can be found in Mina Padi culture. The purpose of this study was to determine the amylolytic bacteria in the waters of the Mina Padi pond. Bacterial isolation began with bacterial sampling, inoculation and isolation of bacteria, calculation of the total abundance of bacteria, observation of bacterial morphology and bacterial purification, and isolation of amylolytic bacteria. The results of the isolation of amylolytic bacteria obtained 3 bacterial isolates capable of producing amylolytic enzymes, namely BA5, BA6, and BA7. The highest index of amylolytic activity was obtained by isolates of BA6 with a medium category of 2.3 cm, and the lowest index was obtained by BA5 with a weak category of 0.3 cm. The average bacterial abundance from each dilution was 2.5 x 103 CFU/mL.
Adamu, K. M., Muhammad, H., Ahmad, S. U., Ahmad, M. M., & Yakubu, A. M. 2020. Diversity of Bacteria and Fungi associated with freshwater fishes from Mijawal River, Nasarawa, Nigeria. Journal of Applied Sciences and Environmental Management, 24(6), 1085-2020. https://doi.org/10.4314/jasem.v24i6.22
Ahmadian, I., Yustiati, A., and Andriani, Y. 2021. Produktivitas Budidaya Sistem Minapadi Untuk Meningkatkan Ketahanan Pangan Di Indonesia : A Review. Jurnal Akuatek, 2(1), 1–6. https://doi.org/10.24198/akuatek.v2i1.33647
Akbar, A. 2017. Peran Intensifikasi Minapadi Dalam Menambah Pendapatan Petani Padi Sawah Digampong Gegarang Kecamatan Jagong Jeget Kabupaten Aceh Tengah. Jurnal S. Pertanian, 1(1), 28–38.
Anbari, I., Fitriadi, R., Nurhafid, M., Palupi, M., dan Riviani. 2022. Isolasi Dan Karakterisasi Bakteri Proteolitik Dari Perairan Sistem Budidaya Minapadi. Jurnal LEMURU, 4(2), 46-56. https://doi.org/10.36526/lemuru.v4i2.2084
Arlita, N.R., Radjasa, O.K., dan Santoso, A. 2013. Identifikasi Pigmen Karotenoid Pada Bakteri Simbion Rumput Laut Caulerpa cupressoides (Vahl) C. Agardh. Journal of Marine Research, 2(3), 68–77. https://doi.org/10.14710/jmr.v2i3.3134
Basitoh, Y.K., Suarsini, E., dan Prabaningtyas, S. 2018. Eksplorasi Bakteri Amilolitik Potensial dari Ranu Pani, Ranu. Jurnal Ilmu Hayat, 3(2), 52-63. http://dx.doi.org/10.17977/um061v3i22019p52-63
Dar, M.A., Pawar, K.D., Jadhav, J.P., and Pandit, R.S. 2015. Isolation of cellulolytic bacteria from the gastro-intestinal tract of Achatina fulica (Gastropoda: Pulmonata) and their evaluation for cellulose biodegradation. International Biodeterioration and Biodegradation, 98, 73–80. https://doi.org/10.1016/j.ibiod.2014.11.016
Dat, T. T. H., Tam, V. T. T., Dung, T. T. K., Bui, L. M., Anh, H. L. T., & Oanh, P. T. T. 2019. Isolation and screening of cellulose and organic matter degrading bacteria from aquaculture ponds for improving water quality in aquaculture. In IOP Conference Series: Earth and Environmental Science, 266, 012002. https://doi.org/10.1088/1755-1315/266/1/012002
Dwidjoseputro, D. 1994. Dasar-Dasar Mikrobiologi. Djambatan. Jakarta.
Elumalai, M., Antunes, C., and Guilhermino, L. 2002. Effects of single metals and their mixtures on selected enzymes of carcinus maenas. Water, Air, and Soil Pollution, 141(1-4), 273–280. https://doi.org/10.1023/A:1021352212089
Hanzen, W.F E., Hastuti, U.S., Makkadafi, S.P., Asna, P.M.Al, dan Nugraheni, F.S.A. 2017. Isolasi dan Identifikasi Bakteri Amilolitik Dari Tanah Yang Tercampur Limbah Kulit Ubi Kayu Di Bondowoso, Jawa Timur. Prosiding Seminar Nasional III Universitas Muhammadiyah Malang, 259–262. https://api.semanticscholar.org/
Harahap, M.S. 2017. Perbandingan Pendapatan Usahatani Minapadi (Padi-Lele) Dan Non Minapadi (Studi Kasus : Desa Sidodadi Ramunia, Kecamatan Beringin, Kabupaten Deli Serdang).[Skripsi], Universitas Muhammadiyah Sumatera Utara Medan.
Hasanah, U., Ardyati, T., and Siswanto, D. 2021. Identification of Sago-Pulp Amylolytic Bacteria and Its Utilization for Granulated Fertilizer. J.Exp. Life Sci, 11(2), 34–42. https://doi.org/10.21776/ub.jels.2021.011.02.02
Isroni, W., Setyawati, D., dan Maulida, N. 2019. Studi Komunitas Bakteri Pada Sistem Resirkulasi Pada Budidaya Lele Dumbo (Clarias Gariepinus). Journal of Aquaculture and Fish Health, 8(3), 159–166. https://doi.org/10.20473/jafh.v8i3.15059
Kadri, A.N., Gelgel, K.T.P., dan Suarjana, I.G.K. 2015. Perbedaan Cara Penyebaran Suspensi terhadap Jumlah Bakteri pada Media Eosin Methylene Blue Agar. Indonesia Medicus Veterinus Juni, 4(3), 205–212. https://ojs.unud.ac.id/index.php/imv/article/download/17499
Kokarkin. 2000. Pemahaman Benar Aplikasi Probiotik. Majalah Trobos.
Kurniasih, T., Lusiastuti, A.M., Azwar, Z.I., dan Melati, I. 2014. Isolasi Dan Seleksi Bakteri Saluran Pencernaan Ikan Lele Sebagai Upaya Mendapatkan Kandidat Probiotik Untuk Efisiensi Pakan Ikan. J. Ris. Akuakultur, 9(1), 99–109. http://dx.doi.org/10.15578/jra.9.1.2014.99-109
Melisha, Harpeni, E., dan Supono. 2016. Produksi Dan Pengujian Aktivitas Amilase Burkholderia Cepacia Terhadap Substrat Yang Berbeda. e-Jurnal Rekayasa dan Teknologi Budidaya Perairan, 5(1), 559–566. http://repository.lppm.unila.ac.id/id/eprint/19107
Mikdarullah, M., dan Nugraha, A. 2017. Teknik Isolasi Bakteri Proteolitik Dari Sumber Air Panas Ciwidey, Bandung. Buletin Teknik Litkayasa Akuakultur, 15(1), 11–14. https://doi.org/10.15578/blta.15.1.2017.11-14
Musharrafieh, R., Tacchi, L., Trujeque, J., LaPatra, S., & Salinas, I. 2014. Staphylococcus warneri, a resident skin commensal of rainbow trout (Oncorhynchus mykiss) with pathobiont characteristics. Veterinary microbiology, 169(1-2), 80-88. https://doi.org/10.1016%2Fj.vetmic.2013.12.012
Nangin, S.R., Langoy, M.L., dan Katili, D. Y. 2015. Makrozoobentos Sebagai Indikator Biologis dalam Menentukan Kualitas Air Sungai Suhuyon Sulawesi Utara. Jurnal MIPA, 4(2), 165. https://doi.org/10.35799/jm.4.2.2015.9515
Nisa, S. K. (2022). Isolasi Dan Penapisan Bakteri Amilolitik Dari Air Dan Sedimen Tambak Udang Vaname (Litopenaeus vannamei) Di Desa Bunton, Kecamatan Adipala, Kabupaten Cilacap. [Skripsi], Universitas Jenderal Soedirman, Purwokerto.
Novitasari, D.T., Purnomo, P.W., Jati, O.E., Ayuningruma, D., dan Sabdaningsih, A. 2021. Skrining Bakteri Penghasil Enzim Amilase Dari Sedimen Tambak Udang Vannamei (Litopenaeus Vannamei). Journal of Fisheries and Marine Research, 5(2), 297–303. https://doi.org/10.21776/ub.jfmr.2021.005.02.15
Nurhafid, M., Syakuri, H., Oedjijono, O., Listiowati, E., Ekasanti, A., Nugrayani, D., dan Pramono, H. 2021. Isolasi dan Identifikasi Molekuler Bakteri Proteolitik dari Saluran Pencernaan Ikan Nila (Oreochromis niloticus) yang Dibudidayakan di Kabupaten Banyumas. Jurnal Perikanan Universitas Gadjah Mada, 23(2), 95. https://doi.org/10.22146/jfs.64072
Palupi, M., Wijaya, R., Chanda, R. A., Azhari, R. F., & Fitriadi, R. 2023. Biodiversity and abundance of phytoplankton in rice-fish farming system. Iraqi Journal of Agricultural Sciences, 54(4), 1084-1093. https://doi.org/10.36103/ijas.v54i4.1800
Pereira, W. A., Mendonça, C. M. N., Urquiza, A. V., Marteinsson, V. íž., LeBlanc, J. G., Cotter, P. D., Villalobos, E. F., Romero, J., & Oliveira, R. P. 2022. Use of probiotic bacteria and bacteriocins as an alternative to antibiotics in aquaculture. Microorganisms, 10(9), 1705. https://doi.org/10.3390/microorganisms10091705
Pokhrel, B., Wanjare, P., and Singh, S. 2015. Isolation, screening and characterization of promising- amylase producing bacteria from sewage enriched soil. Int. Int. J. Adv. Biotechnol. Res., 4(2), 286-290. https://doi.org/10.12983/ijsras-2015-p0159-0165
Prayogo, Rahardja, B.S., Asshanti, A.N., Dewi, N.N., and Santanumurti, M.B. 2018. Exploration of indigenous bacteria in an intensive aquaculture system of African catfish (Clarias sp.) in Banyuwangi, Indonesia. IOP Conf. Series: Earth and Environmental Science, 137(012085), 1–8. https://doi.org/10.1088/1755-1315/137/1/012085
Putra, S.J.W., Nitisupardjo, M., dan Widyorini, N. 2014. Analisis hubungan bahan organik dengan total bakteri pada tambak udang intensif sistem semibioflok di BBPBAP Jepara. Diponegoro Journal of Maquares, 3(3), 121–129. https://doi.org/10.14710/marj.v3i3.6663
Putri, R.A., Nursyirwani, N., and Feliatra, F. 2021. Ability Of Amilolytic Bacteria (Bacillus Paramycoides and Enterobacter Cloacae) In Degrading Organic Materials Of Mangrove Little. Asian Journal of Aquatic Sciences, 4(2), 98–105. https://doi.org/10.31258/ajoas.4.2.98-105
Reddy, N.S., Nimmagadda, A., and Rao, K.R.S.S. 2003. An overview of the microbial α -amylase family. African Journal of Biotechnology, 2(12), 645–648. https://doi.org/10.5897/AJB2003.000-1119
Safrida, Y.D., Yulvizar, C., Devira, C.N., dan Metode, B. 2012. Isolasi dan karakterisasi bakteri berpotensi probiotik pada ikan kembung (Rastrelliger sp.). Depik Jurnal, 1(3), 200–203. https://doi.org/10.13170/depik.1.3.124
Sakamole, E.T., Cyska, L., dan Runtuwene, M. 2014. Pengaruh Pemberian Probiotik Dosis Berbeda dalam Pakan Terhadap Pertumbuhan dan Konversi Pakan Benih Ikan Mas (Cyprinus carpio). Buletin Sariputra, 1(1), 29–33.
Sari, K.D.P., Santoso, L., Efendi, E., dan Harpeni, E. 2017. Potensi Penggunaan Media Teknis sebagai Pengganti Media Sea Water Complete (SWC) untuk Mendukung Pertumbuhan Bakteri Bacillus sp. D2.2. Jurnal Sains Teknologi Akuakultur, 1(2), 95–103. http://jmai.aquasiana.org/index.php/jmai/article/view/13
Setyati, W.A., Martani, E., Triyanto, Subagiyo, dan Zainuddin, M. 2015. Growth kinetics and Protease Activity 36K Isolates Derived from Mangrove Ecosystem Sediment, Karimunjawa, Jepara. Ilmu Kelautan, 20(3), 163–169. https://doi.org/10.14710/ik.ijms.20.3.163-169
Silaban, S., dan Simamora, P. 2018. Isolasi dan Karakterisasi Bakteri Penghasil Amilase dari Sampel Air Tawar Danau Toba. Jurnal Kimia dan Pendidikan, 3(2), 222–230. http://dx.doi.org/10.30870/educhemia.v3i2.3438
Silitonga, L.R., Nursyirwani, dan Effendi, I. 2019. Isolation , Identification And Sensitivity Of Amilolitic Bacteria From Mangrove Ecosystem Sediment In Purnama Marine Station Dumai On The Pathogenic. Asian Journal of Aquatic Sciences, 2(3), 257–266. http://dx.doi.org/10.31258/ajoas.2.3.257-266
Sundari, A.S., Purwani, N.N., and Kurniati, A. 2019. Isolation And Determination Index Amylolytic Of Bacteria From Sediment Mangrove In Wonorejo , Surabaya. QUANTUM: Jurnal Inovasi Pendidikan Sains, 10(1), 38–44. http://dx.doi.org/10.20527/quantum.v10i1.5879
Susilawati, I.O., Batubara, U.M., dan Riany, H. 2015. Analisis Aktivitas Enzim Amilase Yang Berasal Dari Bakteri Tanah Di Kawasan Universitas Jambi. Prosiding Semirata 2015 bidang MIPA BKS-PTN Barat Universitas Tanjungpura Pontianak, 4(1): 359–367. https://jurnal.untan.ac.id/
Suyasa, I.B.O. 2019. Isolasi dan Karakteristik Morfologi Bakteri pada Saluran Pencernaan Ikan Kerapu (Cephalopholis miniata) dari Perairan Kabupaten Klungkung Bali. Meditory, 7(2), 138–143. http://ejournal.poltekkes-denpasar.ac.id/index.php/M
Widiastuti, D., Karima, I.F., dan Setiyani, E. 2019. Efek Antibakteri Sodium Hypochlorite terhadap Staphylococcus aureus Antibacterial Effect of Sodium Hypochlorite to Staphylococcus aureus. Jurnal Ilmiah Kesehatan Masyarakat, 11(16), 302–307. https://doi.org/10.52022/jikm.v11i4.34
Widyati, E. 2008. Peranan Mikroba Tanah pada Kegiatan rehabilitasi lahan Tambang (Roles of Soil Microbes in Ex-Mining Land Rehabilitation). Info Hutan, 5(2), 151–160.
Zuraidah, Wahyuni, D., dan Astuty, E. 2020. Karakteristik Morfologi dan Uji Aktivitas Bakteri Termofilik dari Kawasan Wisata Ie Seuum (Air Panas). Jurnal Ilmu Alam dan Lingkungan, 11(2), 40–47. https://doi.org/10.20956/jal.v11i2.10200
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