The Effect of Andalas (Morus macroura Miq.) Tree Bark Extract on Il-1β Gene Expression in the Pancreas of Hyperglycemic Rats
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Hyperglycemia is a medical condition characterized by elevated blood glucose levels above normal, which is a characteristic of several diseases, particularly diabetes mellitus (DM) and other conditions. In DM, hyperglycemia is associated with inflammation that increases TNF-α and IL-1β cytokines through NF-kB activation. DM treatment with antidiabetic drugs has side effects, such as diarrhea, vomiting, and peripheral edema, necessitating alternative therapies like andalas tree bark extract. This study aimed to determine the effect of andalas tree bark extract (Morus macroura Miq.) on IL-1β gene transcript expression in the pancreas of hyperglycemic rats. This experimental study utilized 24 rats divided into 6 groups: normal control (K-), hyperglycemic control (K+), metformin 500mg control (K), and three treatment groups(P1, P2, P3) with extract doses of 100, 200, and 300 mg/kgBW. Results showed that the mean IL-1β gene concentrations in K-, K+, K, P1, P2, and P3 were 0.09, 1.24, 0.38, 0.12, 1.12, and 1.76 respectively, with significant differences observed (p=0.032). The andalas tree bark extract (Morus macroura Miq.) was most effective at a dose of 100 mg/kg BW for reducing IL-1β expression, while a dose of 200 mg/kg BW was effective in reducing blood glucose levels and increasing body weight in hyperglycemic rats.
Abid, N. Bin, Naseer, M.I., Kim, M.O. 2019. Comparative gene-expression analysis of alzheimer’s disease progression with aging in transgenic mouse model. International Journal of Molecular Sciences. 20.
Antoni, M. 2023. Terapi Diabetes Melitus Tipe 2 Berbasis Hormon Inkretin. Jurnal Kedokteran Meditek. 29.
Bharti, S.K., Krishnan, S., Kumar, Ashwini, Kumar, Awanish 2018. Antidiabetic phytoconstituents and their mode of action on metabolic pathways. Therapeutic advances in endocrinology and metabolism. 9:81–100.
Bucciantini, M., Leri, M., Nardiello, P., Casamenti, F., Stefani, M. 2021. Olive Polyphenols: Antioxidant and Anti-Inflammatory Properties. Antioxidants. 10.
Cortez-Navarrete, M., Pérez-Rubio, K.G., Escobedo-Gutiérrez, M. de J. 2023. Role of Fenugreek, Cinnamon, Curcuma longa, Berberine and Momordica charantia in Type 2 Diabetes Mellitus Treatment: A Review. Pharmaceuticals. 16:515.
Dhankhar, S., Chauhan, S., Mehta, D.K., Nitika, Saini, K., Saini, M., Das, R., Gupta, S., Gautam, V. 2023. Novel targets for potential therapeutic use in Diabetes mellitus. Diabetology & Metabolic Syndrome. 15:17.
Eky Nursia, L.N., Wahyuni Muhsin, S., Maisyaroh Fitri Siregar, S., Kesehatan Masyarakat, P., Kesehatan Masyarakat, F. 2020. Pengaruh Ekstrak Air Selada Laut (Ulva Lactuca) Terhadap Berat Badan Pada Tikus Diabetes. STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa. 13:39–46.
Fajriah, J. 2022. Studi Kasus Asuhan Keperawatan Pada Klien Dengan Diabetes Melitus di Wilayah Kerja Puskesmas Harapan Baru Samarinda. UMKT-DR.
Fauza, M., Febriawan, R. 2023. Suharmanto l Perkembangan Terbaru Pengobatan Herbal Untuk Diabetes Melitus Tipe 2 Agromedicine | 10:106–111.
Harreiter, J., Roden, M. 2023. Diabetes mellitus: definition, classification, diagnosis, screening and prevention (Update 2023). Wiener Klinische Wochenschrift. 135:7–17.
IDF Diabetes Atlas 2021 | IDF Diabetes Atlas n.d. URL https://diabetesatlas.org/atlas/tenth-edition/ (accessed 9.10.23).
Indrawati, R., Sidarningsih, S., Irmawati, A., Fahri, A.S., Tasya, H., Razak, F.A. 2023. The Role of Interleukin 1β (IL-1 β) and Interleukin 6 (IL-6) on Diabetes Mellitus and its Correlation with Cardian Rythm in Wistar Rats (Rattus norvegicus). Malaysian Journal of Medicine and Health Sciences. 19:69–75.
Kil, K., Choi, M.Y., Park, K.H. 2016. In vitro differentiation of human wharton’s jelly- Derived mesenchymal stem cells into auditory hair cells and neurons. Journal of International Advanced Otology. 12.
Kristianingrum, Y.P., Widyarini, S.W.S., Kurniasih, K., Sutrisno, B.S.B., Tabbu, C.R.T.C.R.T.C.R., Sugiyono, S. 2017. Gambaran Histopatologi Otak Tikus Akibat Injeksi Trimetyltin sebagai Model Penyakit Alzheimer. Jurnal Sain Veteriner. 34:84–91.
Kurniawan, D., Syamtoni, I.P., Firmansyah, T., Aisyah, S., Ali, H. 2022. Pengaruh Pemberian Ekstrak Buah Pohon Andalas (Morus macraura Miq.) Terhadap Ekspresi Gen ET-1 Pada Tikus Model Aterosklerosis. Majalah Kedokteran Andalas. 45:575–582.
Monita, Y. 2022. Induksi akar tanaman andalas (Morus macroura Miq.) Dengan berbagai konsentrasi iba secara in vitro.
PERKENI 2021. Guidelines for the Diagnosis and Management of Hyperglycemia in Pregnancy 2021. Pedoman Diagnosis dan Penatalaksanaan Hiperglikemia dalam Kehamilan. 51.
Prameswari, O.M., Widjanarko, S.B. 2014. Uji Efek Ekstrak Air Daun Pandan Wangi Terhadap Penurunan Kadar Glukosa Darah Dan Histopatologi Tikus Diabetes Mellitus [In Press 2014]. Jurnal Pangan dan Agroindustri. 2:16–27.
Saputri, A., Fajriah, S., Cahyana, A. H., Putria, D. K., Saputra, Z., & Angelina, M. 2024. Phytochemical Profiles, Antioxidant, and Anticancer Activities from Leaves and Seeds Extract of Myristica fragrans. Jurnal Kimia Riset, 9(2), 182–195.
Shafiq, Y., Naqvi, S.B.S., Rizwani, G.H., Asghar, Muhammad Arif, Bushra, R., Ghayas, S., Rehman, A.A., Asghar, Muhammad Asif 2021. A mechanistic study on the inhibition of bacterial growth and inflammation by Nerium oleander extract with comprehensive in vivo safety profile. BMC Complementary Medicine and Therapies. 21:1–19.
Shita, A.D.P. 2015. Perubahan Level TNF-α dan IL-1 pada Kondisi Diabetes Mellitus. Prosiding Dentistry Scientific Meeting II, Fakultas Kedokteran Gigi, Universitas Jember. 1–7.
Sisca, D.Y. 2021. Pengaruh pemberian mesenchymal stem cells wharton jelly terhadap ekspresi gen calm1 dan casp9pada tikus alzheimer.
Sun, X., Pang, H., Li, J., Luo, S., Huang, G., Li, X., Xie, Z., Zhou, Z. 2020. The NLRP3 Inflammasome and Its Role in T1DM. Frontiers in Immunology. 11.
T, I.K.S.S.M., Santika, I.W.M. 2023. Kajian Literatur Potensi dan Mekanisme Tanaman Leunca (Solanum nigrum L.) sebagai Nutrasetikal Antiinflamasi. Prosiding Workshop dan Seminar Nasional Farmasi . 2:690–699.
Utara, U.S. 2018. Gambaran Penyembuhan Luka Tikus Diabetes Dengan Fraksi Etil Asetat Daun Binahong ( Anredera cordifolia ( Tenore ) Steenis ) TALENTA Conference Series Gambaran Penyembuhan Luka Tikus Diabetes Dengan Fraksi Etil Asetat Daun Binahong ( Anredera cordifolia ( 1:4–8.
Wang, Xue, Lin, C., Wu, S., Zhang, T., Wang, Y., Jiang, Y., Wang, Xiaohui 2022. Cannabidivarin alleviates neuroinflammation by targeting TLR4 co-receptor MD2 and improves morphine-mediated analgesia. Frontiers in Immunology. 13.
Wiratantri, F. I., Peranginangin, J. M., & Sulaiman, T. N. S. 2024. Optimization of Curcumin Encapsulation Formula with Chitosan and Alginate Using Simplex Lattice Design and Its Effect on Antioxidant Activity. Jurnal Kimia Riset, 9(2), 109– 121.
Yarnita, Y., Rayasari, F., Kamil, A.R. 2023. Program self efficacy dalam perawatan kaki diabetes melitus tipe 2. Jurnal Keperawatan. 15.
Zakaria, A., Yahya, Z., Nurmayunita, H. 2019. Pengaruh pemberian teh daun tin terhadap kadar gula darah pada penderita diabetes mellitus.
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