Difference Time Effect of Equilibration Before Freezing on The Quality of Spermatozoa Sapera Goats using Egg Yellow Tris
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This study aimed to examine the effect of time differences on motility, viability, abnormality, and intact plasma membrane of spermatozoa of Sapera goats before freezing. This study used a Completely Randomized Design (CRD) with three settings, namely the equilibration time P1 (1 h), P2 (3 h), and P3 (4 h), each of six replications. The results of motility using One Way Anova statistical analysis through the Duncan Test were (P1) 71.50 ± 3.94, (P2) 66.00 ± 2.60, and (P3) 60.33 ± 2.42. The results of viability was (P1) 83.00 ± 6.16, (P2) 75.17 ± 4.70, and (P3) 69.00 ± 3.40. Abnormal results was (P1) 2.50 ± 0.55, (P2) 3.33 ± 1.18, and (P3) 6.50 ± 2.88. While the results of intact plasma membranes were (P1) 79.50 ± 8.50, (P2) 72.67 ± 5.28, and (P3) 65.67 ± 3.78. The conclusion of the study showed significant significant results (p<0,05) between the equilibration time and spermatozoa quality of Sapera goats before freezing. The highest presentation of motility, viability and overall plasma membrane was at an equilibration time of 1 h, while the lowest abnormality presentation was at equilibration time of 1 h.
Aerens, C. D., Ihsan, M. N., & Isnaini, N. (2012). Perbedaan Kuantitatif dean Kualitatif Semen Segar pada Berbagai Bangsa Sapi Potong. Malang. Hal: 1-10.
Anggarsari, L. Y. (2015). Pengaruh Waktu Equilibrasi terhadap Motilitas dan Viabilitas Spermatozoa Kambing Boer After Thawing dalam Pengencer yang Mengandung Lisitin Nabati [Skripsi]. Fakultas Kedokteran Hewan Universitas Airlangga. Hal: 9-14.
Afrilia, K. N. (2016). Pengaruh Penambahan L-Arginin dalam Pengencer Susu Skim Kuning Telur Terhadap Motilitas Dan Viabilitas Spermatozoa Domba Merino Post Thawing [Skripsi]. Fakultas Kedokteran Hewan Universitas Airlangga. Hal: 2-7.
Apriyanti, C. (2012). Pengaruh Waktu Ekuilibarsi Terhadap Kualitas Semen Beku Sapi Pesisir Pre dan Post Thawing [Tesis]. Program Studi Ilmu Ternak Universitas Andalas. Hal: 8-16.
Batubara, A., Nasution, S., Subandriyo, I., Inounu, B., Tiesnamurti, & Anggraeni, A. (2016). Kambing Peranakan Etawah (PE). IAARD Press. Jakarta. Hal: 33.
Bearden, H. J., & Fuquay, J. W. (2000). Neuroendocrine regulators of reproduction. Applied Animal Reproduction, 5, 35-53.
Bintara, S. (2011). Rasio spermatozoa X: Y dan kualitas sperma pada kambing kacang dan peranakan ettawa. Sains Peternakan: Jurnal Penelitian Ilmu Peternakan, 9(2), 65-71
Dethan, A. A. (2010). Kualitas dan kuantitas sperma kambing Bligon jantan yang diberi pakan rumput gajah dengan suplementasi tepung darah. [Tesis]. Program Pascasarjana, Universitas Gadjah Mada, Yogyakarta. Hal: 21.
Hafez, E. S. E., & Hafez, B. (2000). Anatomy of male reproduction. In: B. Hafez & E.S.E. Hafez (eds.) Reproduction In Farm Animal. 7th ed. Lippincott & Wilkins, Philadelphia. Pp: 3-12.
Kartina. (2014). Pengaruh Lama Ekuilibrasi Terhadap Daya Hidup Spermatozoa Kambing Peranakan Etawah (PE). [Skripsi]. Universitas Hasanudin. Makassar. Hal: 16.
Kusuma, W. A. (2019). Kajian morfometri spermatozoa kambing saanen dan kambing sapera. [Skripsi]. Universitas Airlangga. Hal: 17.
Krisnawati. (2002). Kualitas dan Kuantitas Semen Segar Kambing Saanen yang Dikoleksi Dari Berbagai Ejakulat. [Skripsi]. Institut Pertaninan Bogor. Bogor. Hal: 5.
Leboeuf, B., Restall, B., & Salamon, S. (2000). Production and storage of goat semen for artificial insemination. Animal Reproduction Science, 62(1-3), 113-141.
Masyitoh, H., Suprayogi, T. W., Praja, R. N., Srianto, P., Madyawati, S. P., & Saputro, A. L. (2018). Persentase Motilitas dan Viabilitas Spermatozoa Kambing Sapera dalam Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Before Freezing. Jurnal Medik Veteriner, 1(3), 105-112.
Muzakkir, D., Wahyuni, S., Akmal, M., & Sabri, M. (2017). Pengaruh Lama Ekuilibrasi terhadap Kualitas Spermatozoa Sapi Aceh Setelah Pembekuan Menggunakan Pengencer Andromed®. Jurnal Ilmiah Peternakan, 5(2), 115-128.
Matshaba, B. (2010). Characterization and cryopreservation of South Africa unimproved indigenous goat semen. Dissertation. Pp: 31.
Ningsih, Z. (2007). Proporsi spermatozoa x dan y kambing peranakan etawa dengan konsentrasi putih telur dan lama inkubasi (Doctoral dissertation, Universitas Islam Negeri Maulana Malik Ibrahim). Hal: 4.
Praharani, L., Adiati, U., & Budiarsana, I. G. M. (2013). Penampilan pertumbuhan anak kambing f-1 anglo nubian peranakan etawah, f-2 sapera, dan peranakan etawah. In Seminar Nasional Teknologi Peternakan dan Veteriner, pp: 301-309.
Pratiwi, S. K. (2014). Identifikasi Morfometri Spermatozoa Kambing Gembrong Dan Domba Garut. [Skripsi]. Universitas Airlangga. Hal: 10.
Penitente-Filho, J. M., Dias, J. C. O., Oliveira, F. A., Silveira, C. O., & Torres, C. A. A. (2017). Correlation between sperm motility and hypoosmotic swelling test on cryopreserved goat semen. Magistra, 27(3/4), 468-473.
Ranjan, R., Goel, A. K., Ramachandran, N., Kharche, S. D., & Jindal, S. K. (2015). Effect of egg yolk levels and equilibration periods on freezability of Jamunapari buck semen. Indian Journal of Small Ruminants, 21(1), 32-36.
Rizal, M., & Herdis. (2005). Daya Hidup Spermatozoa Epididimis Domba Garut yang Dikriopreservasi Menggunakan Modifikasi Pengunakan Pengencer Tris. Hayati, 12(2), 61-66.
Solihati, N., Rasad, S. D., Setiawan, R., Foziah, E. N., & Wigiyanti, E. T. 2018. Semen Quality of Post-Thawed Local Ram's in Tris-Egg Yolk Extender with Different Glutathione Level. In IOP Conference Series: Earth and Environmental Science, 119(1), 012034.
Suryani, E. F. P. (2012). Pengaruh Waktu Equilibrasi Terhadap Motilitas, Viabilitas dan Integritas Membran Spermatozoa Domba Merino dalam Pengencer yang Mengandung Lesitin Nabati. [Skripsi]. Fakultas Kedokteran Hewan Universitas Airlangga. Surabaya. Hal: 11.
Susilowati, S., Hardijanto, Suprayogi, T. W., Sardjito, T., & Hernawati, T. (2010). Penuntun Praktikum Insemnasi Buatan. Fakultas Kedokteran Hewan.
Susilowati, S., Triana, I. N., Wurlina, W., Arimbi, A., Srianto, P., & Mustofa, I. (2019). Addition of L-arginine in skim milk extender maintains goat spermatozoa quality in chilled temperature for five days. Veterinary World, 12(11).
Susilowati, S., Triana, I. N., & Malik, A. (2015). The effects of insulin-like growth factor I (IGF-I) complex from seminal plasma on capacitation, membrane integrity and DNA fragmentation in goat spermatozoa. Asian Pacific Journal of Reproduction, 4(3), 208-211.
Susilawati, T. (2011). Tingkat keberhasilan inseminasi buatan dengan kualitas dan deposisi semen yang berbeda pada sapi Peranakan Ongole. Journal of Tropical Animal Production, 12(2), 15-24.
Syawal, M. (2010). Karakteristik Morfologi Produksi Kambing Boer, Kacang dan Persilangan Pada Umur 0-3 Bulan (Prasapih). Seminar Nasional Teknologi dan Veteriner. pp: 617-619.
Tuhu, A. D., Ondho, Y. S., & Samsudewa, D. (2013). Effect of different water jacket release time in equilibration process on quality semen of java cattle at before freezing and post thawing. Animal Agricultural Journal, 2(1), 466- 477.
Umami, P. L. M., & Bintara, S. (2015). Pengaruh Aras Kuning Kelur Itik Alabio (Anas Platyrhynchos) Dalam Pengencer Tris Fruktosa Terhadap Motilitas, Viabilitas, Dan Abnormalitas Sperma Kambing Bligon Sebelum Dan Sesudah Kriopreservasi. Buletin Peternakan, 39(3), 142-148.
Wibowo, P. A., Astuti, T. Y., & Soediarto, P. (2013). Study of Total Solid (Ts) And Solid Non Fat (Snf) Of Ettawa Cross Goat Milk In One Lactation Period). Jurnal Ilmiah Peternakan, 1(1), 214- 221.
Zulyazaini, D., Wahyuni, S., Akmal, M., & Abdullah, M. A. N. (2016). Karakteristik Semen dan Komposisi Plasma Seminalis Sapi Aceh yang Dipelihara Di BIBD Saree Aceh Besar. Fakultas Kedokteran Hewa Universitas Syiah Kuala. Agripet, 16(2), 121-127.
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