DIFFERENT AMOUNT OF THROMBOCYTES ON BLOOD STORAGE FOR 24 HOURS IN ROOM AND REFRIGERATOR
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Background: Storage is a pre-analytic part that plays a role in maintaining cell quality functionally. Examination of platelet count is influenced by pre-analytic factors, namely time and temperature since specimen collection so that the specimen storage guidelines are needed to obtain accurate laboratory results. Delays in examinations often occur and are caused by the lack of medical personnel, heavy volume of work, or non-technical problems that occur during examination. Purpose: Analyze the effect of storing blood samples at room temperature (18-24oC) and refrigerator (2-8oC) for 24 hours on platelet count. Method: Analytical observations, 30 samples with normal criteria, thrombocytosis, and thrombocytopenia that were examined at Laboratorium Patologi Klinik RSU Haji Surabaya. The method used is analytical observations and the study used a different test of friedman with SPSS 16.0 program to determine whether there is a significant difference in platelet count in blood samples at room temperature and refrigerator temperature for 24 hours with the initial examination. Result: The results of the friedman test analysis of the differences in the results of platelet count for 30 samples showed significant differences with sig. (2-tailed) of 0.00 (p <0.05). Conclusion: There was a significant difference between the results of the platelet count at the beginning of the examination with a delay in the refrigerator storing temperature (2-8oC) and room storing temperature (18-24oC) for 24 hours.
Harjo, Aditya. 2011. Perbedaan Hasil Pemeriksaan Hitung Jumlah Trombosit Cara Manual dan Autometik (Analyzer). Available from: http://digilib.unimus.ac.id. Diakses 29 Desember 2018.
J. A. Child. 2010. Buku Saku Hematologi Klinik. Jakarta: Binarupa Aksara.
Josefsson, E. C, Hartwig., J. H., Hoffmeister K. M. 2007. Platelet Storage Temperature – How Low Can We Go?. USA: Translational Medicine Division, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School.
Kaufman, Richard M. 2006. Platelets: Testing, Dusing and The Storage Lesion Lesion. The Journal of the American Society of Haematology. Available From: http://aseducationbook.hematologylibrary.org. Diakses pada hari Senin, 6 Mei 2019.
Lewis, S.M., Bain, B. J. 2011. Collection and handling of blood. Dacie and Lewis Practical Hematology Vol. 11(10). Pp. 1-10.
Peng, L., Yang, H., Jiang, H., Su, J., Peng, Z. 2001. Automated reticulocyte counting using the Sysmex RAM-1. Clinical and Laboratory Haematology Vol. 23. Pp. 97–102.
Rahmanitarini, A. 2018. Stabilitas Penyimpanan Sampel Pada Pemeriksaan Darah Lengkap. Thesis. Surabaya: Fakultas Kedokteran Universitas Airlangga.
Sadikin, Mohamad. 2001. Biokimia Darah. Jakarta: Penerbit Widya Medika.
Tsuruda, K., Tsuji, T., Usui, T. Kitajima S., Kihara, A., Murai, M., Kasada, Y., Li, Q., Yamada, Y., Kamihira, S. 1999. Evaluation and Clinical Usefulness of the Automated Hematology Analyzer, Sysmex XE 2100, Sysmex. J. Int Vol. 19. Pp. 129-138.
Turhan, T., Sevilay, S., Yucel, C., Koca, Y. 2011. Effects of Storage Conditions on Complete Blood Cell Count Parameters. Turkish Journal of Biochemistry–Turk J Biochem Vol. 36 (2). Pp. 165–174.
Vives-Corrons, J. L., Briggs, C., Simon-Lopez, R., Albarede, S., de la Salle, B., Flegar-Meatrii, Z., Nazor, A., Guyard, A., Lipsic, T., Nagai, Y., Paitu, M., Piqueras, J., Capel, M. J., Van Blerk, M., Wang, J., Marzac, C. 2013. Effect of EDTA-anticoagulated whole blood storage on cell morphology examination. A need for standarization. International Journal of Laboratory Hematology Vol. 36. Pp. 222-226.
Wirawan, R. 2006. Pemeriksaan Laboratorium Hematologi Sederhana. Jakarta: Balai Penerbit Fakultas Kedokteran Indonesia.
Zini, G. 2014. Stability of complete blood count parameters with storage: Toward defined specifications for different diagnostic applications. International Journal of Laboratory Hematology Vol. 36(2). Pp. 111-3.
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