POLYMORPHISM VITAMIN D RECEPTOR GENE (VDR) BSMI (RS1544410) CHRONIC PERIODONTITIS PATIENT IN JAVANESE BANYUMAS ETHNIC

Ryana Budi Purnama; Setiadi Warata Logamarta; Agung Dhartono

doi:10.20473/jvhs.V4.I3.2021.107-113

Authors

Ryana Budi Purnama
ryana.purnama@unsoed.ac.id
Department of Dental Medicine, Faculty of Medicine, Jenderal Soedirman University, Indonesia https://orcid.org/0000-0001-5819-2736
Setiadi Warata Logamarta Department of Dental Medicine, Faculty of Medicine, Jenderal Soedirman University, Indonesia https://orcid.org/0000-0002-7055-9980
Agung Dhartono Department of Dental Medicine, Faculty of Medicine, Jenderal Soedirman University, Indonesia https://orcid.org/0000-0002-7157-8315

Vol. 4 No. 3 (2021): March 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES

Original Research Article

March 31, 2021

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Background: Chronic periodontitis, a chronic inflammatory condition of periodontal tissue, can occur due to microorganisms as a local factor and genetic as a systemic factor. Genetic factors that cause chronic periodontitis, namely mutations in the form of a gene that functions to regulate calcium homeostasis, which is called the vitamin D receptor (VDR) gene. Mutated VDR gene causing different effect in a population and ethnic groups, including Javanese Banyumas Ethnic. Purpose: To understand the genotype distribution and VDR BsmI (rs1544410) gene allotype frequency chronic periodontitis patient in Javanese Banyumas Ethnic. Method: The research was descriptive research in the form of a cross-sectional study of 26 chronic periodontitis patients in the Javanese Banyumas tribe. The samples obtained were carried out by the process of DNA isolation, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP), and data in the Hardy-Weinberg Equilibrium (HWE) Court Lab analysis. Result: Whole samples from DNA isolation amplificated with one ribbon 524 bp VDR gene. RFLP restriction enzyme Bsml process is also showing cutting in each sample. From data analysis, X² is 0,44 with p=0,50 (p>0,05) resulted. It was showing mutant VDR gene allele frequency in chronic periodontitis patients in Javanese Banyumas ethnic were balanced genetically. Conclusion: Genotype distribution in chronic periodontitis patient in Javanese Banyumas ethnic were 20 (76,9%) GG (bb) genotype, 6 (23,1%) GA (Bb) genotype, and 0 (0%) AA (BB) genotype with alel G (b) (wild type) allele frequency 0,88 (88%) and A allele (B or mutant) 0,12 (12%).

Purnama, R. B., Logamarta, S. W., & Dhartono, A. (2021). POLYMORPHISM VITAMIN D RECEPTOR GENE (VDR) BSMI (RS1544410) CHRONIC PERIODONTITIS PATIENT IN JAVANESE BANYUMAS ETHNIC. Journal of Vocational Health Studies, 4(3), 107–113. https://doi.org/10.20473/jvhs.V4.I3.2021.107-113

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