POLYMORPHISM VITAMIN D RECEPTOR GENE (VDR) BSMI (RS1544410) CHRONIC PERIODONTITIS PATIENT IN JAVANESE BANYUMAS ETHNIC

Ryana Budi Purnama, Setiadi Warata Logamarta, Agung Dhartono

= http://dx.doi.org/10.20473/jvhs.V4.I3.2021.107-113
Abstract views = 320 times | downloads = 400 times

Abstract


Background: Chronic periodontitis, a chronic inflammatory condition of periodontal tissue, can occur due to microorganisms as a local factor and genetic as a systemic factor. Genetic factors that cause chronic periodontitis, namely mutations in the form of a gene that functions to regulate calcium homeostasis, which is called the vitamin D receptor (VDR) gene. Mutated VDR gene causing different effect in a population and ethnic groups, including Javanese Banyumas Ethnic. Purpose: To understand the genotype distribution and VDR BsmI (rs1544410) gene allotype frequency chronic periodontitis patient in Javanese Banyumas Ethnic.  Method: The research was descriptive research in the form of a cross-sectional study of 26 chronic periodontitis patients in the Javanese Banyumas tribe. The samples obtained were carried out by the process of DNA isolation, Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP), and data in the Hardy-Weinberg Equilibrium (HWE) Court Lab analysis. Result: Whole samples from DNA isolation amplificated with one ribbon 524 bp VDR gene. RFLP restriction enzyme Bsml process is also showing cutting in each sample. From data analysis, X2 is 0,44 with p=0,50 (p>0,05) resulted. It was showing mutant VDR gene allele frequency in chronic periodontitis patients in Javanese Banyumas ethnic were balanced genetically. Conclusion: Genotype distribution in chronic periodontitis patient in Javanese Banyumas ethnic were 20 (76,9%) GG (bb) genotype, 6 (23,1%) GA (Bb) genotype, and 0 (0%) AA (BB) genotype with alel G (b) (wild type) allele frequency 0,88 (88%) and A allele (B or mutant) 0,12 (12%).


Keywords


BsmI; Chronic periodontitis; Polimorphism; rs1544410; Vitamin D; Receptor Gene

Full Text:

PDF

References


Amin, A., 2014. The Analysis Mutation of the CARD15 Gene Variants in Chronic Periodontitis. Am. J. Clin. Exp. Med. 2, 117.

Borges, M.A.T., de Figueiredo, L.C., de Brito Jr, R.B., Faveri, M., Feres, M., 2009. Microbiological Composition Associated with Vitamin D Receptor Gene Polymorphism in Chronic Periodontitis. Braz. Oral Res. 23, 203–208.

Brett, P.M., Zygogianni, P., Griffiths, G.S., Tomaz, M., Parkar, M., D’Aiuto, F., Tonetti, M., 2005. Functional gene polymorphisms in aggressive and chronic periodontitis. J. Dent. Res. 84, 1149–1153.

de Brito Junior, R.B., Mantuaneli, S.-C.R., Trevilatto, P.C., Souza, A.P., Barros, S.P., 2004. Polymorphisms in The Vitamin D Receptor Gene Are Associated with Periodontal Disease. J. Periodontol. 75, 1090–1095.

de Macêdo, T.C.N., da Conceição N Costa, M., Gomes-Filho, I.S., Vianna, M.I.P., Santos, C.T., 2006. Factors Related to Periodontal Disease in A Rural Population. Braz. Oral Res. 20, 257–262.

Dinas Kesehatan Kabupaten Banyumas, 2011. Profil Kesehatan Kabupaten Banyumas Tahun 2011.

El Jilani, M.M., Mohamed, A.A., Zeglam, H. Ben, Alhudiri, I.M., Ramadan, A.M., Saleh, S.S., Elkabir, M., Amer, I. Ben, Ashammakhi, N., Enattah, N.S., 2015. Association Between Vitamin D Receptor Gene Polymorphisms and Chronic Periodontitis among Libyans. Libyan J. Med. 10.

Fedi, P., Vernono, A., Grey, J., 2004. Silabus Periodonti. EGC.

Gunes, S., Sumer, A.P., Keles, G.C., Kara, N., Koprulu, H., Bagci, H., Bek, Y., 2008. Analysis of vitamin D receptor gene polymorphisms in patients with chronic periodontitis. Indian J. Med. Res. 127, 58–64.

Hamrun Nurlinda, dan H.M., 2011. Polimorfisme Gen Vitamin D Receptor Pada Penderita Periodontitis Kronis. J. Kesehat. 1, 166.

Haussler, M.R., Haussler, C.A., Whitfield, G.K., Hsieh, J.C., Thompson, P.D., Barthel, T.K., Bartik, L., Egan, J.B., Wu, Y., Kubicek, J.L., Lowmiller, C.L., Moffet, E.W., Forster, R.E., Jurutka, P.W., 2010. The nuclear vitamin D receptor controls the expression of genes encoding factors which feed the “Fountain of Youth” to mediate healthful aging. Natl. Institutes Heal. 23, 1–7.

Ho, Y.P., Lin, Y.C., Yang, Y.H., Chou, Y.H., Ho, K.Y., Wu, Y.M., Tsai, C.C., 2017. Association of vitamin D receptor gene polymorphisms and periodontitis in a Taiwanese Han population. J. Dent. Sci. 12, 360–367.

Naito, M., Miyaki, K., Naito, T., Zhang, L., Hoshi, K., Hara, A., Masaki, K., Tohyama, S., Muramatsu, M., Hamajima, N., Nakayama, T., 2007. Association between Vitamin D Receptor Gene Haplotypes and Chronic Periodontitis among Japanese Men. Int. J. Med. Sci. 4, 216–222.

Newman, M., Takei, H., Klokkevold, P., Carranza, F., 2019. Newman and Carranza’s Clinical Periodontology Thirteenth Edition, 13 th. ed.

Setya Ningsih, D., 2015. ubungan Jenis Kelamin Terhadap Kebersihan Rongga Mulut Anak Panti Asuhan. Odonto Dent. J. 2, 14–19.

Uitterlinden, Andre G., Fang, Y., Van Meurs, J.B.J., Pols, H.A.P., Leeuwen, J.P.T.M. Van, 2004. Genetics and Biology of Vitamin D Receptor Polymorphism. Gene 1, 143–156.

Uitterlinden, André G., Fang, Y., Van Meurs, J.B.J., Pols, H.A.P., Van Leeuwen, J.P.T.M., 2004. Genetics and biology of vitamin D receptor polymorphisms. Gene 338, 143–156.

Valdivielso, J.M., Fernandez, E., 2006. Vitamin D Receptor Polymorphisms and Diseases. Clin. Chim. Acta 371, 1–12.

Wajdi, M.N., 2010. Migrasi Antar Pulau di Indonesia. Tesis, Pascasarj. Kependud. dan Ketenagakerjaan Univ. Indones.

Wang, H., Tan, B., Zhaoa, B., Liang, C., Xu, Z., 2013. Raw Data Based Iterative Reconstruction Versus Filtered Back Projection : Image Quality of Low-Dose Chest Computed Tomography Examinations in 87 Patients. Clin. Imaging 37, 1024–1032.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Journal of Vocational Health Studies

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

This Journal is Indexed by: 

                 .


Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.