ANTI-BACTERIAL ACTIVITY TEST OF ETHANOL EXTRACTS AND  ETHLACETATE FRACTION FROM THE EXTRACT OF JATROPHA  CURCAS L. LEAVES AGAINST STAPHYLOCOCCUS AUREUS

Sekar Wulandari; Susanti  Erikania; Vevi  Maritha

doi:10.20473/jvhs.V5.I1.2021.31-38

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Sekar Wulandari
sekarwulandhari73@gmail.com
STIKES Bhakti Husada Mulia Madiun, Madiun, Indonesia https://orcid.org/0000-0002-9050-0089
Susanti Erikania STIKES Bhakti Husada Mulia Madiun, Indonesia
Vevi Maritha STIKES Bhakti Husada Mulia Madiun, Indonesia

Vol. 5 No. 1 (2021): July 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES

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July 31, 2021

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Background: Staphylococcus aureus is a gram-positive bacteria that can cause infection. One of the plants that has antibacterial activity is jatropha leaves which contain flavonoids, saponins, tannins, alkaloids, steroids and polyphenols. Purpose: To determine the antibacterial activity of ethanol extract and ethyl acetate fraction with concentrations of 30%, 60% and 100% against Staphylococcus aureus bacteria. Method: The method for extracting jatropha leaves is maceration with 96% ethanol solvent and the fractionation method, namely liquid-liquid fractionation with ethyl acetate solvent. Antibacterial activity test was carried out in vitro with the disc diffusion method and compared the mean zone of inhibition of each treatment with a positive control (gentamicin 10 μg). Result: The results showed that the ethanol extract and ethyl acetate fraction of jatropha leaves had a strong resistance response, while the positive control gave a very strong inhibitory response to the growth of S. aureus bacteria. Based on the one way ANOVA test, ethanol extract and ethyl acetate fraction showed a significant difference from each treatment with a significant value (P=<0.05). Conclusion: The ethanol extract of Jatropha leaves can inhibit the growth of S. aureus bacteria at a concentration of 100% (18.28 ± 0.50 mm), 100% concentration of ethyl acetate fraction (15.10 ± 0.12 mm). The ethanol extract provided the best inhibition power, namely 18.28 ± 0.50 mm and a positive control 21.82 ± 0.092 mm.

Wulandari, S., Erikania, S. ., & Maritha, . V. . (2021). ANTI-BACTERIAL ACTIVITY TEST OF ETHANOL EXTRACTS AND ETHLACETATE FRACTION FROM THE EXTRACT OF JATROPHA CURCAS L. LEAVES AGAINST STAPHYLOCOCCUS AUREUS. Journal of Vocational Health Studies, 5(1), 31–38. https://doi.org/10.20473/jvhs.V5.I1.2021.31-38

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