ANTIBACTERIAL ACTIVITY OF MARIGOLD FLOWER (TAGETES ERECTA L.) ETHANOL EXTRACT CREAM AGAINST STAPHYLOCOCCUS AUREUS

Putu Lakustini Cahyaningrum; A.A A Sauca Sunia Widyantari

doi:10.20473/jvhs.V6.I3.2023.165-172

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Putu Lakustini Cahyaningrum
nining@unhi.ac.id
Ayurvedic Health Study Program, Faculty of Health, Hindu University, Indonesia https://orcid.org/0000-0002-9528-6931
A.A A Sauca Sunia Widyantari Biology Study Program, Faculty of Information Technology and Science, Hindu University, Indonesia https://orcid.org/0000-0001-8912-5050

Vol. 6 No. 3 (2023): March 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES

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March 30, 2023

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Background: Acne is caused by inflammation and obstruction of the skin pores, which traps excess oil (acne vulgaris). The bacterium Staphylococcus aureus is the causative agent of acne. Treatment and prevention efforts can be made traditionally. Purpose: Testing the antibacterial efficacy of the marigold flower ethanol extract cream (Tagetes erecta L.) against Staphylococcus aureus. Method: This type of research is descriptive exploratory and experimental. Samples of ethanol extract from marigold flowers (Tagetes erecta L.) were formulated into three treatments, namely concentrations of 25%, 50%, and 100% with three replications. Antibacterial efficacy testing was carried out by the agar well diffusion method. Data analysis used One-way ANOVA and Tukey HSD tests. Result: The inhibition zones produced by the ethanolic extract of marigold flowers were 8.59 ± 0.047, 9.23 ± 0.026, and 10.23 ± 0.044 respectively. Thus, a higher concentration of the ethanolic extract of marigold flowers in the cream produced more significant inhibitory activity. Conclusion: The ethanol extract cream of marigold flower (Tagetes erecta L.) showed antibacterial activity against Staphylococcus aureus and is suitable to be applied as a cream for the traditional treatment of acne (acne vulgaris).

Cahyaningrum, P. L., & Widyantari, A. A. S. S. (2023). ANTIBACTERIAL ACTIVITY OF MARIGOLD FLOWER (TAGETES ERECTA L.) ETHANOL EXTRACT CREAM AGAINST STAPHYLOCOCCUS AUREUS. Journal of Vocational Health Studies, 6(3), 165–172. https://doi.org/10.20473/jvhs.V6.I3.2023.165-172

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Balouiri, M., Sadiki, M., Ibnsouda, S.K., 2016. Methods for In Vitro Evaluating Antimicrobial Activity: A Review. J. Pharm. Anal. Vol. 6(2), Pp. 71-79.

Burlec, A.F., Hancianu, M., Macovei, I., Mircea, C., Fifere, A., Turin-Moleavin, I.-A., Tuchilus, C., Robu, S., Corciova, A., 2022. Eco-Friendly Synthesis and Comparative In Vitro Biological Evaluation of Silver Nanoparticles using Tagetes erecta Flower Extracts. Appl. Sci. Vol. 12(2), Pp. 1-20.

Cahyaningrum, P.L., Yuliari, S.A.M., Mediastari, A.P.A., 2020. Efektivitas Antibakteri Sediaan Sabun Bunga Gemitir (Tagetes erecta L.) terhadap Staphylococcus aureus dan Escherichia coli. J. Muhammadiyah Med. Lab. Technol. Vol. 3(2), Pp. 11-24.

Chau, T.P., Muthusamy, M., Chinnathambi, A., Alahmadi, T.A., Kuppusamy, S., 2021. Optimization of Extraction and Quantification of Flavonoids from Averrhoa Bilimbi Fruits using RP-HPLC and Its Correlation between Total Flavonoids Content against Antimicrobial Activity. Appl. Nanosci. Pp. 1-8.

D'Epenoux, L.R., Guillouzouic, A., Bemer, P., Dagnelie, M.-A., Khammari, A., Dreno, B., Corvec, S., 2022. Should We Consider Broad"Spectrum Quinolone Antibacterial Agent as Acne Treatment in The Antimicrobial Resistance Era? J. Eur. Acad. Dermatology Venereol. Vol. 36(3), Pp. e193-e195.

Darwin, M., Mamondol, M.R., Sormin, S.A., Nurhayati, Y., Tambunan, H., Sylvia, D., Adnyana, M.D.M., Prasetiyo, B., Vianitati, P., Gebang, A.A., 2021. Quantitative Approach Research Method, 1st ed. CV. Media Sains Indonesia, Bandung-Jawa Barat.

Edy, H.J., Hadimartono, M., Wahyuono, S., Nugroho, A.E., 2019. Testing of Antibacterial Activity Hydrogel Ethanol Extract of Tagetes erecta L. J. MIPA Vol. 8(3), Pp. 96-98.

Edy, H.J., Parwanto, M.L.E., 2019. Pemanfaatan Tanaman Tagetes erecta Linn. dalam Kesehatan. J. Biomedika dan Kesehat. Vol. 2(2), Pp. 77-80.

Faraz, T., Rizwani, G.H., Hina, B., Sharif, H., 2020. Pharmacognostic, Antimicrobial and Toxicological Studies of a Seasonal Medicinal Plant; Tagetes Patula L. J. Pharm. Pharm. Sci. Vol. 8(4), Pp. 242-247.

Fu, X.-Q., Zhang, G.-L., Deng, L., Dang, Y.-Y., 2019. Simultaneous Extraction and Enrichment of Polyphenol and Lutein from Marigold (Tagetes erecta L.) Flower by An Enzyme-Assisted Ethanol/Ammonium Sulfate System. Food Funct. Vol. 10(1), Pp. 266-276.

Greydanus, D.E., Azmeh, R., Cabral, M.D., Dickson, C.A., Patel, D.R., 2020. Acne in The First Three Decades of Life: An Update of A Disorder with Profound Implications for All Decades of Life. Dis. Mon. Vol. 67(4), Pp. 101103.

Kosmadaki, M., Katsambas, A., 2017. Topical Treatments for Acne. Clin. Dermatology Vol. 35(2), Pp. 173-178

Kresnapati, I.N.B.A., Khaerunnisa, S., Safitri, I., 2021. Ethanol Extract of Marigold Flower (Tagetes Erecta L.) Decreases the Total Cholesterol, Low Density Lypoprotein (LDL), Malondialdehyde (MDA), and Apoliprotein B (APOB) on Hyperlipidemia Rat Models. Folia Medica Indones. Vol. 57(3), Pp. 245-249.

Kumar, A., Scholar, D.R., Sai, S., Jhade, D., 2021. Cosmetic Potential of Selected Medicinal Plants: A Review. J. Pharmacogn. Phytochem. Vol. 10(4), Pp. 381-386.

Likasari, I.D., Astuti, R.W., Yahya, A., Isnaini, N., Purwiandono, G., Hidayat, H., Wicaksono, W.P., Fatimah, I., 2021. NiO Nanoparticles Synthesized by using Tagetes erecta L. leaf Extract and Their Activities for Photocatalysis, Electrochemical Sensing, and Antibacterial Features. Chem. Phys. Lett. Vol. 780, Pp. 138914.

Mandias, I.I., Yamlean, P., Abdullah, S.S., 2022. Formulation and Antibacterial Assessment of Peel-Off Gel Mask Ethanol Extracts of Avocole Leaves (Persea americana Mill.) against Staphylococcus epidermidis Bacteria as Anti-Acne. PHARMACON Vol. 11(4), Pp. 1813-1824.

Mekjaruskul, C., Kumkarnjana, S., Fangkrathok, N., Kengkittipat, W., Sirichat, N., 2021. Potential Cosmeceutical Applications and Evaluation of Human Skin Irritation of Tagetes erecta L. Flower Extract. Pharmacognosy Res. Vol. 13(4), Pp. 199-207.

Mekvimol, T., Poonthona, G., Chaipunna, C., Pumipuntu, N., 2020. Antimicrobial Activity of Marigold (Tagetes erecta), Mulberry (Morus indica), and Red Shallot (Allium ascalonicum) Extracts against Streptococcus agalactiae. Int. J. One Heal. Vol. 6(1), Pp. 56-60.

Meurer, M.C., Mees, M., Mariano, L.N.B., Boeing, T., Somensi, L.B., Mariott, M., Silva, R. de C.M.V. de A.F. da, Santos, A.C. Dos, Longo, B., França, T.C.S., Klein-Junior, L.C., Souza, P. de, Andrade, S.F. de, Silva, L.M. da, 2019. Hydroalcoholic Extract of Tagetes erecta L. Flowers, Rich in The Carotenoid Lutein, Attenuates Inflammatory Cytokine Secretion and Improves The Oxidative Stress in An Animal Model of Ulcerative Colitis. Nutr. Res. Vol. 66, Pp. 95-106.

Mertha Adnyana, I.M.D., Sudaryati, N.L.G., 2023. Phytochemical Analysis of the Antioxidant Compounds of Baper Tea and Its Potential as an Immunomodulatory agent and Candidate for Standardized Herbal Medicine. Trends in Sciences Vol. 20, Pp. 6391.

Moline, C., Barros, L., Dias, M.I., Lopez, V., Langa, E., Ferreira, I.C.F.R., Gomez-Rincon, C., 2018. Edible Flowers of Tagetes erecta L. as Functional Ingredients: Phenolic Composition, Antioxidant and Protective Effects on Caenorhabditis elegan. Nutrients Vol. 10(12), Pp. 242-247.

Padalia, H., Chand, S., 2015. Antimicrobial Efficacy of Different Solvent Extracts of Tagetes erecta L. Flower, Alone and in Combination with Antibiotics. Appl. Microbiol. Vol. 1(1), Pp. 1-10.

Parwanto, M.L.E., Lambertus, M., Tjahyadi, D., Jaya, H., 2020. Efficcacy of Tagetes erecta linn. Leaf Extract Cream on Rat Dermal Wound Healing. Int. J. Pharm. Res. Vol. 13(1), Pp. 364-374.

Perez-Ortega, G., Angeles-Lopez, G.E., Argueta-Villamar, A., Gonzalez-Trujano, M.E., 2017. Preclinical Evidence of The Anxiolytic and Sedative-Like Activities of Tagetes erecta L. Reinforces Its Ethnobotanical Approach. Biomed. Pharmacother. Vol. 93, Pp. 383-390.

Pramitha, D.A.I., Sibarani, J., Suaniti, M., 2017. Sifat Fisikokimia Hand and Body Cream dengan Pemanfaatan Ekstrak Etanol Bunga Gemitir (Tagetes erecta L.) dan Bunga Pacar Air Merah (Impatiens balsamina L.) dari Limbah Canang. Cakra Kim. (Indonesian E-Journal Appl. Chem. Vol. 5(1), Pp. 1-11.

Priani, S.E., Dewi, W.K., Gadri, A., 2018. Formulasi Sediaan Mikroemulsi Gel Anti Jerawat Mengandung Kombinasi Minyak Jinten Hitam (Nigella sativa L.) dan Minyak Zaitun (Olea europaea L.). Kartika J. Ilm. Farm. Vol. 6(2), Pp. 57-64.

Puluh, E.A., Edy, H.J., Siampa, J.P., 2019. Uji Anti-Bakteri Sediaan Masker Peel off Ekstrak Etanol Daun Alpukat (Persea ameicana Mill.) terhadap Bakteri Staphylococcus epidermidis sebagai Anti-Jerawat. J. MIPA Vol. 8(3), Pp. 101-104.

Rajkumari, J., Choudhury, Y., Bhattacharjee, K., Pandey, P., 2021. Rhizodegradation of Pyrene by a Non-pathogenic Klebsiella pneumoniae Isolate Applied with Tagetes erecta L. and Changes in the Rhizobacterial Community. Front. Microbiol. Vol. 23, Pp. 593023.

Romes, N.B., Wahab, R.A., Hamid, M.A., 2021. The Role of Bioactive Phytoconstituents-Loaded Nanoemulsions for Skin Improvement: A Review. Biotechnol. Biotechnol. Equip. Vol. 35(1), Pp. 721-729.

Safar, A.A., Ghafoor, A., Dastan, D., 2020. Screening of Chemical Characterization, Antifungal and Cytotoxic Activities of Essential Oil Constituents of Tagetes erecta l. from Erbil, Kurdistan Region-Iraq. Polish J. Environ. Stud. Vol. 29(3), Pp. 1-10.

Salehi, B., Valussi, M., Morais-Braga, M.F.B., Carneiro, J.N.P., Leal, A.L.A.B., Coutinho, H.D.M., Vitalini, S., Kregiel, D., Antolak, H., Sharifi-Rad, M., Sharifi-Rad, J., 2018. Tagetes spp. Essential oils and Other Extracts: Chemical Characterization and Biological Activity. Molecules Vol. 23(11), Pp. 1-35.

Selvam, S.I., Joicesky, S.M.B., Dashli, A.A., Vinothini, A., Premkumar, K., 2021. Assessment of Antibacterial, Anti inflammation and Wound Healing Activity in Wistar Albino Rats using Green Silver Nanoparticles Synthesized from Tagetes erecta Leaves. J. Appl. Nat. Sci. Vol. 13(1), Pp. 343–351.

Singh, Y., Gupta, A., Kannojia, P., 2020. Tagetes erecta (Marigold)-A Review on Its Phytochemical and Medicinal Properties Pharmacological Activity of Cow Urine. Curr. Med. Drug Res. Vol. 4(1), Pp. 1-6.

Trinh, P.-C., Thao, L.-T.-T., Ha, H.-T.-V., Nguyen, T., 2020. DPPH-Scavenging and Antimicrobial Activities of Asteraceae Medicinal Plants on Uropathogenic Bacteria. Evidence-based Complement. Altern. Med. Pp.1-9.

Tyagi, P.K., Tyagi, S., Gola, D., Arya, A., Ayatollahi, S.A., Alshehri, M.M., Sharifi-Rad, J., 2021. Ascorbic Acid and Polyphenols Mediated Green Synthesis of Silver Nanoparticles from Tagetes erecta L. Aqueous Leaf Extract and Studied Their Antioxidant Properties. J. Nanomater. Pp. 1-9.

Ventura-Martinez, R., Angeles-Lopez, G.E., Rodriguez, R., Gonzalez-Trujano, M.E., Deciga-Campos, M., 2018. Spasmolytic Effect of Aqueous Extract of Tagetes erecta L. Flowers is Mediated Through Calcium Channel Blockade on The Guinea-Pig Ileum. Biomed. Pharmacother. Vol. 103, Pp. 1552-1556.

Verma, V., Joshi, C.P., Agarwal, A., Soni, S., Kataria, U., 2020. A Review on Pharmacological Aspects of Pyrimidine Derivatives. J. Drug Deliv. Ther. Vol. 10(5), Pp. 358-361.

Walsh, T.R., Efthimiou, J., Dreno, B., 2016. Systematic Review of Antibiotic Resistance in Acne: An Increasing Topical and Oral Threat. Lancet Infect. Dis. Vol. 16(3), Pp. e23-e33.

Widjanarko, D.A., Simaremare, E.S.S., Pratiwi, R.D., 2021. Formulation and Activity Test of Anti-acne Gel Extract of Tuber Lily (Crinum asiaticum L.) against Staphylococcus Aureus Bacteria In Vitro. J. Adv. Bio- Pharm. Pharmacovigil. Vol. 3(2), Pp. 128-132.

Zanovello, M., Mariano, L.N.B., Cechinel-Zanchett, C.C., Boeing, T., Tazinaffo, G.C., Silva, L.M. da, Silva, D.B., Junior, A.G., Souza, P. de, 2021. Tagetes erecta L. Flowers, A Medicinal Plant Traditionally used to Promote Diuresis, induced Diuretic and Natriuretic Effects in Normotensive and Hypertensive Rats. J. Ethnopharmacol. Vol. 279, Pp. 114393.

Zhang, Huali, Zhang, S., Zhang, Hua, Chen, X., Liang, F., Qin, H., Zhang, Y., Cong, R., Xin, H., Zhang, Z., 2020. Carotenoid Metabolite and Transcriptome Dynamics Underlying Flower Color in Marigold (Tagetes erecta L.). Sci. Rep.