THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIA

Chori  Khotul Ula; Edy Haryanto Edy Haryanto; Syamsul Arifin Syamsul Arifin; Wisnu   Istanto

doi:10.20473/jvhs.V7.I1.2023.10-16

Authors

Chori Khotul Ula
ulalala76@gmail.com
Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, Indonesia https://orcid.org/0009-0005-3024-2531
Edy Haryanto Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, Indonesia https://orcid.org/0009-0002-8269-9283
Syamsul Arifin Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, Indonesia https://orcid.org/0000-0002-0319-2605
Wisnu Istanto Department of Medical Laboratory Technology, Health Polytechnic Ministry of Health Surabaya, Indonesia https://orcid.org/0000-0001-8392-0272

Vol. 7 No. 1 (2023): July 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES

Original Research Article

July 30, 2023

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Background: The pre-analytic stage has the most significant contribution to error, which is 60% to 70%. This is an excellent contribution to the dependability of laboratory results. Delays in the examination often occur in the laboratory due to various factors influencing glucose examination outcomes. Purpose: To ascertain the effect of long delays in various temperatures on the results of plasma NaF and serum glucose tests in diabetes mellitus patients who have hypercholesterolemia. Method: Pre-test and post-test design used in a pre-experimental method. In the pretest, plasma NaF and serum glucose levels were checked immediately. In the posttest, the plasma NaF and serum samples were delayed for 4 and 8 hours at the temperature of the refrigerator (4°C) and room temperature (25°C). Result: The results of One-way ANOVA test analysis of the plasma NaF glucose level with an immediate examination, a delay of 4 and 8 hours at room temperature (25°C) showed no effect with a Sig. of 0.423 > 0.05. Plasma NaF glucose level with an immediate examination, 4 and 8 hours delay in refrigerator temperature (4°C) showed no effect with Sig. of 0.772 > 0.05. Serum glucose levels with an immediate examination, 4 and 8 hours delay at room temperature (25°C) showed no effect with a Sig. of 0.333 > 0.05. Serum glucose levels with an immediate examination, 4 and 8 hours delay in refrigerator temperature (4°C) showed no effect with a Sig. of 0.604 > 0.05. Conclusion: There was no effect of 4 hours and 8 hours of delay at the temperature of the refrigerator (4°C) and room temperature (25°C) on the results of NaF and serum glucose examinations in patients with diabetes mellitus who have hypercholesterolemia.

Khotul Ula, C. ., Edy Haryanto, E. H., Syamsul Arifin, S. A., & Istanto, W. . (2023). THE EFFECT OF DELAY IN TEMPERATURE VARIATIONS ON RESULTS OF GLUCOSE TEST IN DM PATIENTS WITH HYPERCHOLESTEROLEMIA. Journal of Vocational Health Studies, 7(1), 10–16. https://doi.org/10.20473/jvhs.V7.I1.2023.10-16

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Agung, A., Retnoningrum, D., KSL, I.E., 2017. Perbedaan Kadar Glukosa Serum dan Plasma Natrium Fluorida (Naf) dengan Penundaan Pemeriksaan. J. Kedokt. Diponegoro Vol. 6(2), Pp. 188-195.

Apriani, A., Umami, A., 2018. Perbedaan Kadar Glukosa Darah pada Plasma EDTA dan Serum dengan Penundaan Pemeriksaan. J. Vokasi Kesehat. Vol. 4(1), Pp. 19-22.

Fahmi, N.F., Firdaus, N., Putri, N., 2020. Pengaruh Waktu Penundaan terhadap Kadar Glukosa Darah Sewaktu dengan Metode Poct pada Mahasiswa. Nurs. Updat. J.Ilm. Ilmu Keperawatan Vol. 11(2), Pp. 1-11.

Kasimo, E.R., 2020. Perbedaan Glukosa Serum dan Plasma NaF dengan Penundaan 12 Jam pada Pasien Diabetes Melitus. J. Kedokt. dan Kesehat. Vol. 16(1), Pp. 20-24.

Mariady, F., Sugiarto, C., Sadeli, L., 2013. Perbandingan Hasil Pemeriksaan Kadar Glukosa Darah Sewaktu Menggunakan Glukometer dan Spektrofotometer Pada Penderita Diabetes Melitus di Klinik Nirlaba Bandung. Universitas Kristen Maranatha.

Moe, M.O., Okstad, W., Berland, S., Berland, S.B., 2018. Effects of Storage Duration and Temperature Conditions on Biochemical Analytes in Porcine Clotted. J. Dairy, Vet. Anim. Res. Vol. 7(1), Pp. 1-6.

Noviyanti, F., Decroli, E., Sastri, S., 2015. Perbedaan Kadar LDL-kolesterol pada Pasien Diabetes Melitus Tipe 2 dengan dan tanpa Hipertensi di RS Dr . M .Djamil Padang Tahun 2011. J. Kesehat. Andalas Vol. 4(2), Pp. 20-24.

Nugraha, G., Badrawi, I., 2018. Pedoman Teknik Pemeriksaan Laboratorium Klinik. Trans Info Media, Jakarta.

Nurhayati, E., Suwono, S., Fiki, E.N., 2017. Penggunaan Antikoagulan Naf pada Pengukuran Kadar Glukosa Darah Selama 2 Jam. J. Lab. Khatulistiwa Vol. 1(1), Pp. 33-39.

Permana, H., 2017. Komplikasi Kronik dan Penyakit Penyerita pada Diabetes. Universitas Padjajaran.

Putra, A.L., Wowor, P.M., Wungouw, H.I.S., 2015. Gambaran Kadar Gula Darah Sewaktu Pada Mahasiswa Angkatan 2015 Fakultas Kedokteran Universitas Sam Ratulangi Manado. J. e-Biomedik 3.

Ramadhani, Q.A.N., Garini, A., Nurhayati, Harianja, S.H., 2019. Perbedaan Kadar Glukosa Darah Sewaktu menggunakan Serum dan Plasma Edta. J. Kesehat. Poltekkes Palembang Vol. 14(2), Pp. 80-84.

Sacher, R.A., McPherson, R.A., 2012. Tinjauan Klinis Hasil Pemeriksaan Laboratorium. Edisi 11. EGC, Jakarta.

Santi, O.D., Rosita, L., Cahyaningrum, Y.D., 2011. Pengaruh Suhu dan Interval Waktu Penyimpanan Sampel Serum pada Pengukuran Kadar Glukosa Darah. JKKI (Jurnal Kedokt. dan Kesehat. Indones. Vol. 3(8), Pp. 39-43.

Sasmita, B., Yaswir, R., Lillah, H., Desywar, D., 2020. Perubahan Kadar Glukosa Plasma Naf dan Serum yang Disimpan Antara Suhu Ruangan dan Suhu 2-8˚C. Program Pendidikan Dokter Spesialis IBagian Patologi Klikik FK UNAND/RSUP. DR. M.DJAMIL Padang.

Sholikin, M., 2018. Perbedaan Kadar Glukosa Darah Puasa Penyimpanan Suhu 2-8°C berdasarkan Waktu Pemeriksaan. Universitas Muhammadiyah Semarang.

Siregar, M.T., Wulan, W.S., Setiawan, D., Nuryati, A., 2018. Kendali Mutu Pusat Pendidikan Sumber Daya Manusia Kesehatan Badan Pengembangan dan Pemberdayaan Sumber Manusia Kesehatan. Indonesia.

Stoll, C., Wolkers, W.F., 2011. Membrane Stability during Biopreservation of Blood Cells. Transfus. Med. Hemotherapy J. Vol. 38(2), Pp. 89-97.

Sujono, S., Maulida, Y.A., Sari, M.P., 2016. Kadar Protein Total dan Ureum dengan dan tanpa Penambahan γ-cyclodextrin pada Serum Lipemik. Clin. Chem. Vol. 5(1), Pp. 16-19.

Susiwati, S., 2018. Perbedaan Kadar Glukosa Darah Puasa Pasien Diabetes Melitus Tipe 2 pada Plasma Naf Berdasarkan Waktu Pemeriksaan di Rsud Dr. M.Yunus Provinsi Bengkulu Tahun 2017. J. Nurs.Public Heal. Vol. 6(1), Pp. 82–87.

Trisyani, N., Djasang, S., Armah, Z., 2020. Perbandingan Kadar Glukosa Darah Pada Sampel Yang Mengalami Variasi Lama Penundaan Pemisahan. J. Media Anal. Kesehat. 11, 34.

Tyas, L.C., 2015. Pemeriksaan Kadar Glukosa Darah yang Diperiksa secara Langsung dan Ditunda 24 Jam. Jombang.

WHO, 2013. Diagnostic Criteria and Classification of Hyperglycaemia First Detected in Pregnancy.

Yuliana, A., 2018. Buku Ajar Biokimia Farmasi. Jakad Media Publishing, Jakarta.

Yuni, R.Y.R., Anggraini, H., Ariyadi, T., 2019. Pengaruh Lama Penyimpanan Plasma NaF Terhadap Kadar Glukosa Darah Sewaktu. Universitas Muhammadiyah Semarang.