MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIA

Swapna C. Senan; Jithu  Paul Jacob; Ramani  Bhai

doi:10.20473/jvhs.V7.I2.2023.83-89

Authors

Swapna C. Senan Department of Microbiology, Sree Gokulam Medical College, Trivandrum, Kerala https://orcid.org/0000-0001-9949-9785
Jithu Paul Jacob
jithupaul007@gmail.com
Research Department of Fisheries and Aquaculture, St. Albert’s College (Autonomous), Ernakulam, Kerala, India
Ramani Bhai Department of Microbiology, Sree Gokulam Medical College, Trivandrum, Kerala

Vol. 7 No. 2 (2023): November 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES

Original Research Article

November 11, 2023

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Background: Isolates of Klebsiella pneumoniae resistant to Carbapenem appears to be most commonly transmitted by hospitalized patients. This may cause infections which lead to mortality. Therefore, studies on molecular level will help to gain more information and be able to know their cross-transmission and resistance mechanisms among these isolates showing resistance. Purpose: Investigate the molecular characterization of carbapenem-resistant Klebsiella pneumoniae isolates from various clinical samples. Klebsiella pneumoniae are aerobic and Gram-negative bacilli found in the gastrointestinal tract of humans and other animals and also as saprophytes in soil. Method: In this study, 401 bacteria of Klebsiella isolates were taken from various clinical samples according to standard protocol. Carbapenem-resistant genes of Klebsiella pneumoniae (CRK) isolates were detected using multiplex polymerase chain reaction (PCR). Result: The twelve isolates resistant to carbapenem were confirmed phenotypically and were confirmed using a multiplex Polymerase Chain Reaction. Conclusion: The present study concluded that identification of Carbapenem-resistant genes of Klebsiella pneumoniae (KPC) could help find out the routes of dissemination of the species and may control its spread within the hospital premises.

C. Senan, S., Paul Jacob, J. ., & Bhai, R. . (2023). MOLECULAR CHARACTERIZATION OF CARBAPENEM RESISTANT KLEBSIELLA PNEUMONIAE AT A TERTIARY CARE LABORATORY IN KERALA, INDIA. Journal of Vocational Health Studies, 7(2), 83–89. https://doi.org/10.20473/jvhs.V7.I2.2023.83-89

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