MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356

Shafira Indriyani Chusnadia; Atik  Kurniawati; Sari  Setyaningsih; Erawati  Wulandari; Pudji  Astuti

doi:10.20473/jvhs.V7.I2.2023.115-120

Authors

Shafira Indriyani Chusnadia Oral Biology Department, Faculty of Dentistry, Jember University, Indonesia https://orcid.org/0009-0009-3821-7348
Atik Kurniawati
atik.fkg@unej.ac.id
Oral Biology Department, Faculty of Dentistry, Jember University, Indonesia https://orcid.org/0000-0003-4731-6320
Sari Setyaningsih Biomedic Department, Faculty of Dentistry, Jember University, Indonesia https://orcid.org/0000-0002-7300-3734
Erawati Wulandari Conservative Dentistry Department, Faculty of Dentistry, Jember University, Indonesia https://orcid.org/0000-0001-9268-5200
Pudji Astuti Biomedic Department, Faculty of Dentistry, Jember University, Indonesia https://orcid.org/0009-0000-7526-1983

Vol. 7 No. 2 (2023): November 2023 | JOURNAL OF VOCATIONAL HEALTH STUDIES

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Background: Dental caries is an oral cavity disease that affects most Indonesians. Lactobacillus acidophilus (L. acidophilus) is one of the bacteria that causes dental caries. Control of bacteria in the form of antibacterial agents is needed to suppress the growth of L. acidophilus. Purple leaves (Graptophyllum pictum L. Griff) are a medicinal plant with antibacterial compounds, namely flavonoids, alkaloids, saponins, and tannins. Purpose: Determine the Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus. Method: The sample consisted of seven groups, including positive control (chlorhexidine 0.2%), negative control (BHI-B), and purple leaves extract with concentrations of 25%, 12.5%, 6.25%, 3.12%, and 1.56%. The antibacterial activity of purple leaves extract was carried out quantitatively using a spectrophotometer with a wavelength of 600 nm. After that, it was incubated at 37°C for 48 hours, followed by absorbance measurement. The absorbance results were then analyzed using the Paired T-Test (before and after incubation). Result: Purple leaves extract concentrations of 6.25%, 12.5%, and 25% had an inhibitory effect on L. acidophillus. Conclusion: Minimum Inhibitory Concentration (MIC) of purple leaves extract on the growth of L. acidophilus was 6.25%

Indriyani Chusnadia, S., Kurniawati, A. ., Setyaningsih, S., Wulandari, E. ., & Astuti, P. . (2023). MINIMUM INHIBITORY CONCENTRATION OF PURPLE LEAF EXTRACT (GRAPTOPHYLLUM PICTUM L. GRIFF) AGAINST LACTOBACILLUS ACIDOPHILUS ATCC 4356. Journal of Vocational Health Studies, 7(2), 115–120. https://doi.org/10.20473/jvhs.V7.I2.2023.115-120

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