The potency of Immunoglobulin Y anti Porphyromonas gingivalis to inhibit the adherence ability of Porphyromonas gingivalis on enterocytes

Nova Andriani Hepitaria, Indeswati Diyatri, Markus Budi Rahardjo, Rini Devijanti Ridwan

= http://dx.doi.org/10.20473/j.djmkg.v53.i1.p20-23
Abstract views = 633 times | downloads = 296 times

Abstract


Background: Pophyromonas gingivalis (P. gingivalis) bacteria are the main type of bacterium that cause chronic periodontitis. Immunoglobulin Y (IgY) is a type of immunoglobulin found in poultry, such as chickens and birds. IgY can be used as an alternative method of preventing the accumulation of plaque that causes chronic periodontitis. Purpose: To determine the ability of IgY anti P. gingivalis to inhibit adherence of P. gingivalis. Methods: The samples were divided into eight groups, each group containing 10 ml of IgY anti P. gingivalis and 50 ml of enterocyte cells. The control group contained 50 ml of IgY anti P. gingivalis, and 50 ml of enterocyte cells. Serial dilution was carried out to the first seven groups, with the first group containing 90 ml phosphate-buffered saline (PBS) and 10 ml IgY anti P. gingivalis, and the second to seventh groups containing 50 ml PBS before adding 50 ml of enterocyte cells and 50 ml of bacterial suspension per group. The number of bacteria was calculated as an adherence index value using a light microscope. Results: This study shows that IgY anti P. gingivalis significantly reduces the adherence index value of P. gingivalis. Conclusion: IgY anti P. gingivalis has potency to inhibit the adherence of P. gingivalis.


Keywords


adherence; egg yolk; IgY; Pophyromonas gingivalis

Full Text:

PDF

References


Vargas Segura AI, Ilyina A, Segura Ceniceros EP, Silva Belmares Y, Méndez González L. Etiology and microbiology of periodontal diseases: a review. African J Microbiol Res. 2015; 9(48): 2300–6.

Aljehani YA. Risk factors of periodontal disease: review of the literature. Int J Dent. 2014; 2014: 1–9.

Ismail AD. Oral bacterial interactions in periodontal health and disease. J Dent Oral Hyg. 2014; 6(5): 51–7.

How KY, Song KP, Chan KG. Porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line. Front Microbiol. 2016; 7: 1–14.

Sandle T. Bacterial adhesion: an introduction. IVT Nerwork. 2013; : 1–8.

Nami Y, Haghshenas B, Abdullah N, Barzegari A, Radiah D, Rosli R, Khosroushahi AY. Probiotics or antibiotics: Future challenges in medicine. J Med Microbiol. 2015; 64(2): 137–46.

Baloch AR, Zhang XY, Schade R. IgY Technology in aquaculture - a review. Rev Aquac. 2015; 7(3): 153–60.

Bachtiar EW, Afdhal A, Meidyawati R, Soejoedono RD, Poerwaningsih E. Effect of topical anti-Streptococcus mutans IgY gel on quantity of S. mutans on rats’ tooth surface. Acta Microbiol Immunol Hung. 2016; 63(2): 159–69.

Munhoz LS, Vargas GDÁ, Fischer G, Lima M de, Esteves PA, Ḧbner S de O. Avian IgY antibodies: characteristics and applications in immunodiagnostic. Cienc Rural St Maria. 2014; 44(1): 153–60.

Rahman S, Van Nguyen S, Icatlo FC, Umeda K, Kodama Y. Oral passive IgY-based immunotherapeutics: A novel solution for prevention and treatment of alimentary tract diseases. Hum Vaccines Immunother. 2013; 9(5): 1039–48.

Wicaksono AW, Setiawatie EM, Rubianto M. Daya hambat ekstrak buah mengkudu (Morinda citrifolia L.) terhadap pertumbuhan bakteri Porphyromonas gingivalis. Thesis. Universitas Airlangga: Surabaya; 2013. p. 3.

Hidayati DYN, Santosaningsih D, Khotimah H. Characterization of Pili protein as cell adhesion molecules of mycobacterium tuberculosis H37RV to mouse enterocytes: in vitro study. Int J Res Rev. 2018; 5(4): 66–72.

Reynolds J. Serial dilution protocols. Am Soc Microbiol. 2005; (September 2005): 1–7.

Ridwan RD. The role of Actinobacillus actinomycetemcomitans fimbrial adhesin on MMP-8 activity in aggressive periodontitis pathogenesis. Dent J (Majalah Kedokt Gigi). 2012; 45(4): 181–6.

Thu HM, Myat TW, Win MM, Thant KZ, Rahman S, Umeda K, Van Nguyen S, Icatlo FC, Higo-Moriguchi K, Taniguchi K, Tsuji T, Oguma K, Kim SJ, Bae HS, Choi HJ. Chicken egg yolk antibodies (IgY) for prophylaxis and treatment of rotavirus diarrhea in human and animal neonates: a concise review. Korean J Food Sci Anim Resour. 2017; 37(1): 1–9.

Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F. Monoclonal versus polyclonal antibodies: distinguishing characteristics, applications, and information resources. ILAR J. 2005; 46(3): 258–68.

Chalghoumi R, Beckers Y, Portetelle D, Théwis A. Hen egg yolk antibodies (IgY), production and use for passive immunization against bacterial enteric infections in chicken: a review. Biotechnol Agron Soc Env. 2009; 13(2): 295–308.

Pandit N, Changela R, Bali D, Tikoo P, Gugnani S. Porphyromonas gingivalis: Its virulence and vaccine. J Int Clin Dent Res Organ. 2015; 7(1): 51.

Gani BA, Chismirina S, Hayati Z, B EW, Bachtiar BM, Wibawan IWT. The ability of IgY to recognize surface proteins of Streptococcus mutans. Dent J (Majalah Kedokt Gigi). 2009; 42(4): 189–93.

Garrett TR, Bhakoo M, Zhang Z. Bacterial adhesion and biofilms on surfaces. Prog Nat Sci. 2008; 18(9): 1049–56.

Hasan A, Palmer RM. A clinical guide to periodontology: Pathology of periodontal disease. Vol. 216, British Dental Journal. Nature Publishing Group; 2014. p. 457–61.


Refbacks

  • There are currently no refbacks.


View My Stats