The activity of polyclonal IgY derived from Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis in inhibiting colonization of Fusobacterium nucleatum and Streptococcus sanguinis

Oktaviani Suci Lestari, Rini Devijanti Ridwan, Tuti Kusumaningsih, S. Sidarningsih

= http://dx.doi.org/10.20473/j.djmkg.v52.i2.p81-85
Abstract views = 29 times | views = 6 times

Abstract


Background: Fusobacterium nucleatum (F. nucleatum) and Streptococcus sanguinis (S. sanguinis) play a role in dental plaque formation which leads to periodontitis. Immunoglobulin Y (IgY) is present in both serum and egg yolk and can bind to the surface components of bacteria. F. nucleatum and S. sanguinis feature the same type of IV pili as Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). Saliva binding protein (SsaB) in S. sanguinis is a FimA homolog. FimA constitutes a surface element of Porphyromonas gingivalis (P. gingivalis). F. nucleatum and P. gingivalis possess the same outer membrane protein (OMP) molecular mass. Purpose: The study aimed to determine the activity of A. actinomycetemcomitans and P. gingivalis polyclonal IgY present in serum and egg yolk that can inhibit colonization of F. nucleatum and S. sanguinis. Methods: IgY samples were diluted with phosphate buffer saline (PBS). Several holes were made in the nutrient medium with 10 μl antigen (F. nucleatum/S. sanguinis) being inserted into the center hole. 10 μl PBS, 1:1, 1:2, 1:4, 1:8, 1:16 A. actinomycetemcomitans or P. gingivalis polyclonal IgY were subsequently introduced into the surrounding holes. The results of incubation at 37°C were observed after 24-48 hours. Kruskal Wallis and MannWhitney tests were administered to analyse the data. Results: A. actinomycetemcomitans and P. gingivalis polyclonal IgY groups in serum showed a precipitation line at dilution ratios of 1:1 and 1:2, whereas in egg yolk this occurred only at a 1:1 dilution ratio with F. nucleatum and S. sanguinis bacteria in this study. No significant differences were evident between each dilution (p>0.05) and none existed between serum and egg yolk (p>0.05). Conclusion: IgY polyclonal of A. actinomycetemcomitans and P. gingivalis in both serum and egg yolk initiate activities that can inhibit colonization of F. nucleatum and S. sanguinis.

Keywords


Aggregatibacter actinomycetemcomitans; Fusobacterium nucleatum; IgY; Porphyromonas gingivalis; Streptococcus sanguinis

Full Text:

PDF

References


Han K-J, Shin S-C. A clinical study on the influence of immunoglobulin Y-containing chewing gum on the periodontium. Int J Clin Prev Dent. 2016; 12(1): 37–44.

Xu Y, Selerio-Poely T, Ye X. Clinical and microbiological effects of egg yolk antibody against Porphyromonas gingivalis as an adjunct in the treatment of moderate to severe chronic periodontitis: a randomized placebo-controlled clinical trial. J Periodontal Implant Sci. 2018; 48(1): 47–59.

Safitri CI, Soeroso Y, Sunarto H, Pontoh DS, Bachtiar B. Association of salivary count of streptococcus sanguinis with the periodontal status of coronary heart disease patients: a quantitative study. J Int Dent Med Res. 2017; 10(Special issue): 782–8.

Stingu C-S, Eschrich K, Rodloff AC, Schaumann R, Jentsch H. Periodontitis is associated with a loss of colonization by Streptococcus sanguinis. J Med Microbiol. 2008; 57(4): 495–9.

Hayati M, Herman H, Rezano A. The effect of probiotic Lactobacillus casei supplementation on the secretory immunoglobulin A level in the saliva of wistar rats. Bali Med J. 2018; 7(3): 727–31.

Kapoor A, Malhotra R, Grover V, Grover D. Systemic antibiotic therapy in periodontics. Dent Res J (Isfahan). 2012; 9(5): 505–15.

Kumar SB. Chlorhexidine mouthwash- a review. J Pharm Sci Res. 2017; 9(9): 1450–2.

Rahman S, Van Nguyen S, Icatlo Jr. FC, Umeda K, Kodama Y. Oral passive IgY-based immunotherapeutics. Hum Vaccin Immunother. 2013; 9(5): 1039–48.

Cahyaningsih T. Aplikasi IgY spesifik Staphylococcus aureus sebagai pencegahan Staphylococcosis pada kelinci. Thesis. Bogor: Institut Pertanian Bogor; 2016. p. 19.

Zeynalian H, Kazerouni F, Ebrahimi F, Rahimipour A, Bakhshi M, Samadi R. Assessment of the safety of chicken egg yolk antibody (IgY) consumption by measuring the activity of antioxidant enzymes (superoxide dismutase‚ catalase‚ glutathione peroxidase) and malondialdehyde concentration as a lipid peroxidation marker in mice. J Paramed Sci. 2017; 8(3): 29–35.

Amro WA, Al-Qaisi W, Al-Razem F. Production and purification of IgY antibodies from chicken egg yolk. J Genet Eng Biotechnol. 2018; 16(1): 99–103.

Barati B, Ebrahimi F, Nazarian S. Egg yolk antibodies for disease prevention. J Bacteriol Mycol. 2016; 3(2): 219–22.

Chalghoumi R, Beckers Y, Portetelle D, Théwis A. Hen egg yolk antibodies (IgY), production and use for passive immunization against bacterial enteric infections in chicken: a review. Biotechnol Agron Soc Env. 2009; 13(2): 295–308.

Kinane DF, Mombelli A. Periodontal disease. Basel: Karger; 2012. p. 12.

Kline KA, Fälker S, Dahlberg S, Normark S, Henriques-Normark B. Bacterial adhesins in host-microbe interactions. Cell Host Microbe. 2009; 5(6): 580–92.

Zijnge V, Ammann T, Thurnheer T, Gmür R. Subgingival biofilm structure. Front Oral Biol. 2011; 15: 1–16.

Hospenthal MK, Costa T, Waksman G. Pilus biogenesis at the inner and outer membranes of Gram-negative bacteria. Nature Reviews Microbiology. 2017.

InterPro. Adhesion lipoprotein (IPR006128). 2018. Available from: https://www.ebi.ac.uk/interpro/entry/IPR006128. Accessed 2018 Sep 25.

Namikoshi J, Maeba S, Yamamoto M, Hayakawa M, Abiko Y, Otake S. Nasal immunization with P. gingivalis surface protein antigen and cholera toxin adjuvant induces T helper 2 responses in both mucosal and systemic compartments. Int J Oral-Medical Sci. 2003; 1(2): 90–6.

Sharon I, Gomez LA, Vani C, Michael A. Isolation and characterization of butanol-tolerant Staphylococcus aureus using Japanese quail. Int J Life Sci Pharma Res. 2016; 6(4): 45–52.

HiMedia Laboratories. HiPer® Ouchterlony Double Diffusion Teaching Kit (Antibody Titration). Mumbai: HiMedia; 2012. p. 1–6.

Thermo Scientific. Immunodiffusion plates. Rockford: Thermo Fisher Scientific Inc.; 2011. p. 1–3.

Lee EN, Sunwoo HH, Menninen K, Sim JS. In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium. Poult Sci. 2002; 81(5): 632–41.

Naidu AS. Natural food antimicrobial systems. Florida: CRC Press; 2000. p. 235.

Salazar LF, Jayasinghe U. Double diffusion test in gels (Ouchterlony). In: Techniques in plant virology. Lima, Peru: International Potato Center; 2018. p. 1–3.


Refbacks

  • There are currently no refbacks.


View My Stats