Effects of 3% Mobe (Artocarpus lakoocha) leaf extract gel on the post-extraction socket: In-vivo study
Downloads
Background: Mobe (Artocarpus lakoocha) is recognized for its potential in accelerating wound healing, attributed to its secondary metabolites. However, its impact on hard tissue healing on post- extraction tooth sockets has been underexplored. Purpose: This study aims to analyze the effects of 3% Mobe leaf extract gel on the number of fibroblasts, osteoblasts, osteocytes and post-extraction sockets in Wistar rats. Methods: Thirty-two male Wistar rats had their left mandibular incisors extracted and were divided into eight groups. Mobe leaf extract gel was applied to Group I-IV and Aloclair® gel was applied to Group V-VIII for 14 days, twice a day. Residual socket volume (RSV) and fibroblast counts were measured on days 3, 7, and 14, while osteoblast and osteocyte counts were assessed on days 7, 14, and 28 post extraction. The RSV data were analyzed using repeated measures analysis of variance (ANOVA) and post-hoc least significant difference (LSD) test, while fibroblasts, osteoblasts, and osteocytes counts were analyzed with one-way ANOVA and post-hoc LSD tests. Results: RSV decreased significantly in both groups on day 14 (p=0.001 and p=0.002); however, the RSV was lower on the Mobe group. The fibroblast counts were higher in the Mobe group (p=0.001), and there was a significant difference in the mean number of osteoblasts and osteocytes in the Mobe group (p= 0.043 and p=0.008). Conclusion: The study concludes that 3% Mobe leaf gel extract is better than Aloclair® in accelerating socket healing mainly due to increased proliferation of fibroblasts, osteoblasts, and osteocytes.
Downloads
Badan Penelitian dan Pengembangan Kesehatan. Laporan nasional riset kesehatan dasar 2018. Jakarta: Kementerian Kesehatan Republik Indonesia; 2018. p. 249–68. web: http://repository.bkpk.kemkes.go.id/3514/
Ather S, Harding KG, Tate SJ. Wound management and dressings. In: Rajendran S, editor. Advanced textiles for wound care. 2nd ed. Elsevier; 2019. p. 1–22. doi: https://doi.org/10.1016/B978-0-08-102192-7.00001-1
Kurniawati A, Kristanti YD, Rahmat NA, Rahayu YC, Cholid Z, Sosiawan A. The role of purple leaves extract (Graptophyllum Pictum (L.) Griff) on the number of fibroblasts and blood vessels in the socket after tooth extraction. Dent J. 2024; 57(1): 56–61. doi: https://doi.org/10.20473/j.djmkg.v57.i1.p56-61
Li M, Zhang A, Li J, Zhou J, Zheng Y, Zhang C, Xia D, Mao H, Zhao J. Osteoblast/fibroblast coculture derived bioactive ECM with unique matrisome profile facilitates bone regeneration. Bioact Mater. 2020; 5(4): 938–48. doi: https://doi.org/10.1016/j.bioactmat.2020.06.017
Florencio-Silva R, Sasso GR da S, Sasso-Cerri E, Simíµes MJ, Cerri PS. Biology of bone tissue: structure, function, and factors that influence bone cells. Biomed Res Int. 2015; 2015: 421746. doi: https://doi.org/10.1155/2015/421746
Hassumi JS, Mulinari-Santos G, Fabris AL da S, Jacob RGM, Gonçalves A, Rossi AC, Freire AR, Faverani LP, Okamoto R. Alveolar bone healing in rats: micro-CT, immunohistochemical and molecular analysis. J Appl Oral Sci. 2018; 26: e20170326. doi: https://doi.org/10.1590/1678-7757-2017-0326
Bainbridge P. Wound healing and the role of fibroblasts. J Wound Care. 2013; 22(8): 407–8, 410–2. doi: https://doi.org/10.12968/jowc.2013.22.8.407
Vieira AE, Repeke CE, De Barros Ferreira S, Colavite PM, Biguetti CC, Oliveira RC, Assis GF, Taga R, Trombone APF, Garlet GP. Intramembranous bone healing process subsequent to tooth extraction in mice: micro-computed tomography, histomorphometric and molecular characterization. PLoS One. 2015; 10(5): 1–22. doi: https://doi.org/10.1371/journal.pone.0128021
Dean R. The periodontal ligament: development, anatomy and function. J Oral Heal Dent Manag. 2017; 16(6): 1–7. web: https://www.oralhealth.ro/volumes/2017/volume-6/Paper1008.pdf
Brune T, Borel A, Gilbert TW, Franceschi JP, Badylak SF, Sommer P. In vitro comparison of human fibroblasts from intact and ruptured ACL for use in tissue engineering. Eur Cells Mater. 2007; 14(0): 78–90. doi: https://doi.org/10.22203/ecm.v014a08
Pollard TD, Earnshaw WC, Lippincott-Schwartz J, Johnson G. Cells of the extracellular matrix and immune system. In: Cell Biology. 3rd ed. Elsevier; 2017. p. 491–504. doi: https://doi.org/10.1016/B978-0-323-34126-4.00028-1
Mescher AL. Bone. In: Junqueira's basic histology: Text & atlas. 16th ed. McGraw-Hill Education; 2019. p. 142. web: https://accessmedicine.mhmedical.com/content.aspx?bookid=3047§ionid=255120777
Mohindra M, Jain JK. Fundamentals of orthopedics. New Delhi: Jaypee Brothers Medical Publishers; 2016. p. 7, 16, 17. doi: https://doi.org/10.5005/jp/books/12657
Orsted HL, Keast DH, Forest-Lalande L, Kuhnke JL, O'Sullivan-Drombolis D, Jin S, Haley J, Evans R. Skin: Anatomy, physiology and wound healing. In: Foundations of best practice for skin and wound management. Wounds Canada; 2018. p. 19–23. web: https://www.woundscanada.ca/docman/public/health-care-professional/bpr-workshop/166-wc-bpr-skin-physiology/file
Vettori E, Costantinides F, Nicolin V, Rizzo R, Perinetti G, Maglione M, Di Lenarda R. Factors influencing the onset of intra- and post- operative complications following tooth exodontia: Retrospective survey on 1701 patients. Antibiotics. 2019; 8(4): 264. doi: https://doi.org/10.3390/antibiotics8040264
Sinclair Pharmaceuticals Limited. Aloclair® plus gel - Information leaflet. 2012. Available from: https://www.chemist.net/media/catalog/product/file/Aloclair_Plus_Gel_ds9361_1.pdf.
Adriani CR, Rosidah, Dalimunthe A, Satria D. Wound healing activity of ointment artocapus lakoocha roxb. ethanol leaves extract evaluation on excision wound. J Innov Pharm Biol Sci. 2019; 6(1): 12–4. web: https://jipbs.com/index.php/journal/article/view/353
Yeh C-J, Chen C-C, Leu Y-L, Lin M-W, Chiu M-M, Wang S-H. The effects of artocarpin on wound healing: in vitro and in vivo studies. Sci Rep. 2017; 7(1): 15599. doi: https://doi.org/10.1038/s41598-017-15876-7
Hanafiah OA, Satria D, Avi Syafitri. Effects of 1% and 3% Mobe leaf extract gel on socket wound healing after tooth extraction. Dentika Dent J. 2021; 24(1): 1–5. doi: https://doi.org/10.32734/dentika.v24i1.5327
Anwar F, Przybylski R. Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.). Acta Sci Pol Technol Aliment. 2012; 11(3): 293–301. pubmed: http://www.ncbi.nlm.nih.gov/pubmed/22744950
Institutional Animal Care and Use Committee. Anesthesia (Guideline) | Vertebrate Animal Research. Iowa, USA: The University of Iowa; 2020. Available from: https://animal.research.uiowa.edu/iacuc-guidelines-anesthesia. Accessed 2021 Feb 2.
Luthfi M, Juliastuti WS, Risky YA, Wijayanti EH, Rachmawati AE, Asyhari NPO. Expression of fibroblast cells after extraction of wistar rat teeth after topical application of okra fruit (Abelmoschus esculentus) gel. Infect Dis Rep. 2020; 12(Suppl 1): 8726. doi: https://doi.org/10.4081/idr.2020.8726
Mozzati M, Gallesio G, di Romana S, Bergamasco L, Pol R. Efficacy of plasma-rich growth factor in the healing of postextraction sockets in patients affected by insulin-dependent diabetes mellitus. J Oral Maxillofac Surg. 2014; 72(3): 456–62. doi: https://doi.org/10.1016/j.joms.2013.10.010
Savi FM, Brierly GI, Baldwin J, Theodoropoulos C, Woodruff MA. Comparison of different decalcification methods using rat mandibles as a model. J Histochem Cytochem. 2017; 65(12): 705–22. doi: https://doi.org/10.1369/0022155417733708
Slaoui M, Bauchet A-L, Fiette L. Tissue sampling and processing for histopathology evaluation. Methods Mol Biol. 2017; 1641: 101–14. doi: https://doi.org/10.1007/978-1-4939-7172-5_4
Sarkarat F, Modarresi A, Chiniforush N, Yazdanparast L, Rakhshan V. Efficacy of photodynamic therapy in minimizing bisphosphonate-related osteonecrosis of the jaws after dental extraction: A preliminary animal study. J Oral Maxillofac Surg. 2019; 77(2): 307–14. doi: https://doi.org/10.1016/j.joms.2018.09.036
Hupp JR, Edward E, Tucker MR. Contemporary oral and maxillofacial surgery. Philadelpia: Elsivier; 2019. p. 45–51, 134. web: https://books.google.co.id/books?id=LmzWDwAAQBAJ
Andreasen JO, Andreasen FM, Andersson L. Textbook and color atlas of traumatic injuries to the teeth. 5th ed. Wiley-Blackwell; 2019. p. 4–9, 15–16, 182–183, 188. web: https://www.wiley.com/en-us/Textbook+and+Color+Atlas+of+Traumatic+Injuries+to+the+Teeth,+5th+Edition-p-9781119167051
Kumar GS. Bone. In: Orban's oral histology & embryology. 14th ed. India: Elsevier; 2015. p. 169–72, 180–2, e6. web: https://shop.elsevier.com/books/orbans-oral-histology-and-embryology/kumar/978-81-312-4033-5
Paul W, Sharma CP. Advances in wound healing materials: Science and skin engineering. Smithers Rapra Technology Ltd; 2015. p. 1–165. web: https://books.google.co.id/books/about/Advances_in_Wound_Healing_Materials.html?id=Tvk5nwEACAAJ&redir_esc=y
Miranda LL, Guimarí£es-Lopes V de P, Altoé LS, Sarandy MM, Melo FCSA, Novaes RD, Gonçalves RV. Plant extracts in the bone repair process: A systematic review. Mediators Inflamm. 2019; 2019: 1296153. doi: https://doi.org/10.1155/2019/1296153
Siddiqui JA, Partridge NC. Physiological bone remodeling: Systemic regulation and growth factor involvement. Physiology (Bethesda). 2016; 31(3): 233–45. doi: https://doi.org/10.1152/physiol.00061.2014
Hakim A. Diversity of secondary metabolites from Genus Artocarpus (Moraceae). Nusant Biosci. 2010; 2(3): 146–56. doi: https://doi.org/10.13057/nusbiosci/n020307
Mukwaya E, Xu F, Wong M-S, Zhang Y. Chinese herbal medicine for bone health. Pharm Biol. 2014; 52(9): 1223–8. doi: https://doi.org/10.3109/13880209.2014.884606
Nneka Alaribe F, Razwinani M, Maepa M, Shirley Caroline Motaung K. The potential effect of medicinal plants for cartilage regeneration. In: Cartilage tissue engineering and regeneration techniques. IntechOpen; 2019. p. 1–11. doi: https://doi.org/10.5772/intechopen.84780
Nesa ML, Munira S, Bristy AS, Islam M, Chayan H, Rashid M. Cytotoxic, anti-inflammatory, analgesic, CNS depressant, antidiarrhoeal activities of the methanolic extract of the Artocarpus Lakoocha leaves. World J Pharm Sci. 2015; 3(2): 167–74. web: https://wjpsonline.com/index.php/wjps/article/view/cytotoxic-analgesic-antidiarrhoeal-artocarpus-lakoocha-leaves
Hutabarat ESH, Allo JSL, Winanto TR, Mulyawan I, Kamadjaja DB. Effects of lime (Citrus aurantifolia christm. swingle) peel extract on fibroblast proliferation and angiogenesis in rat's tooth extraction sockets. Biochem Cell Arch. 2019; 19(Suppl. 2): 4767–70. web: https://www.connectjournals.com/pages/articledetails/toc030671
Sari Y, Purnawan I, Kurniawan DW, Sutrisna E. A comparative study of the effects of Nigella sativa oil gel and Aloe vera gel on wound healing in diabetic rats. J Evidence-Based Integr Med. 2018; 23: 2515690X1877280. doi: https://doi.org/10.1177/2515690X18772804
Copyright (c) 2024 Dental Journal
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
- Every manuscript submitted to must observe the policy and terms set by the Dental Journal (Majalah Kedokteran Gigi).
- Publication rights to manuscript content published by the Dental Journal (Majalah Kedokteran Gigi) is owned by the journal with the consent and approval of the author(s) concerned.
- Full texts of electronically published manuscripts can be accessed free of charge and used according to the license shown below.
- The Dental Journal (Majalah Kedokteran Gigi) is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License
