Analysis of anti-Streptococcus sanguinis IgY ability to inhibit Streptococcus sanguinis adherence

Suryani Hutomo, Heni Susilowati, Dewi Agustina, Widya Asmara

= http://dx.doi.org/10.20473/j.djmkg.v51.i1.p33-36
Abstract views = 357 times | views = 80 times

Abstract


Background: Streptococcus sanguinis (S. sanguinis), an oral commensal bacterium, is often implicated in infective endocarditis. Its adherence to the tooth surface is the initial step in dental plaque formation. In addition to the important role of S. sanguinis in systemic disease and antimicrobial resistance, it is necessary to develop methods to control dental plaque formation. Immunoglobulin Y (IgY) has been used to prevent bacterial infection. Purpose: The purpose of this study is to analyze the ability of anti-S. sanguinis IgY antibodies to inhibit S. sanguinis adherence to hydroxyapatite (HA) discs as a model of the tooth surface. Methods: Antibodies were produced by immunizing hens with S. sanguinis suspension. Boosters were given three times following the first injection. An agar gel precipitation test (AGPT) was used to detect the presence of anti-S. sanguinis IgY. A bacterial adherence assay was performed twice to analyze the ability of IgY and the optimal concentration required to inhibit bacterial adherence. Results: The formation of a precipitation line using AGPT confirmed the presence of the antibody. In addition, it was shown that the anti-S. sanguinis IgY antibody could inhibit bacterial adherence to HA. Statistical analysis using One-way ANOVA revealed a significant difference in the optical density (OD) value between the groups (p<0.05). The results of electron microscopy scanning confirmed the quantitative analysis by means of a bacterial adherence test. Conclusion: Anti-S. sanguinis IgY has the ability to inhibit adherence of S. sanguinis to HA discs at an optimal concentration of 30%. The inhibitive effect was stronger in the presence of saliva.

Keywords


Streptococcus sanguinis; IgY; bacterial adherence

Full Text:

PDF

References


Kreth J, Merritt J, Qi F. Bacterial and host interactions of oral streptococci. DNA Cell Biol. 2009; 28(8): 397–403.

Okahashi N, Nakata M, Sakurai A, Terao Y, Hoshino T, Yamaguchi M, Isoda R, Sumitomo T, Nakano K, Kawabata S, Ooshima T. Pili of oral Streptococcus sanguinis bind to fibronectin and contribute to cell adhesion. Biochem Biophys Res Commun. 2010; 391(2): 1192–6.

Díaz PI, Kolenbrander PE. Subgingival biofilm communities in health and disease. Rev Clínica Periodoncia, Implantol Rehabil Oral. 2009; 2(3): 187–92.

Westling K. Viridans group Streptococci septicaemia and endocarditis. Thesis. Stockholm: Karolinska Institutet; 2005. p. 9-31.

Ekdahl C. Infective endocarditis – aspects of pathophysiology, epidemiology, management and prognosis. Thesis. Linköping: Linköping University; 2008. p. 7-36.

Lockhart PB, Brennan MT, Sasser HC, Fox PC, Paster BJ, Bahrani-Mougeot FK. Bacteremia associated with toothbrushing and dental extraction. Circulation. 2008; 117(24): 3118–25.

Masuda K, Nemoto H, Nakano K, Naka S, Nomura R, Ooshima T. Amoxicillin-resistant oral streptococci identified in dental plaque specimens from healthy Japanese adults. J Cardiol. 2012; 59(3): 285–90.

Sahasrabhojane P, Galloway-Peña J, Velazquez L, Saldaña M, Horstmann N, Tarrand J, Shelburne SA. Species-level assessment of the molecular basis of fluoroquinolone resistance among viridans group streptococci causing bacteraemia in cancer patients. Int J Antimicrob Agents. 2014; 43(6): 558–62.

Wen J, Zhao S, He D, Yang Y, Li Y, Zhu S. Preparation and characterization of egg yolk immunoglobulin Y specific to influenza B virus. Antiviral Res. 2012; 93(1): 154–9.

Xu Y, Li X, Jin L, Zhen Y, Lu Y, Li S, You J, Wang L. Application of chicken egg yolk immunoglobulins in the control of terrestrial and aquatic animal diseases: a review. Biotechnol Adv. 2011; 29(6): 860–8.

Chalghoumi R, Beckers Y, Portetelle D, Théwis A. Hen egg yolk antibodies (IgY), production and use for passive immunization against bacterial enteric infections in chicken: a review. Biotechnol Agron Soc Env. 2009; 13(2): 295–308.

Vanessa B, Virginie M, Nathalie Q, Marie-Hélène R, Christine I. Hartmannella vermiformis can promote proliferation of Candida spp. in tap-water. Water Res. 2012; 46(17): 5707–14.

Siswomihardjo W, Sunarintyas S, Tontowi AE. The effect of zirconia in hydroxyapatite on Staphylococcus epidermidis growth. Int J Biomater. 2012; 2012: 1–4.

Johansen T, Agdestein A, Olsen I, Nilsen S, Holstad G, Djønne B. Biofilm formation by Mycobacterium avium isolates originating from humans, swine and birds. BMC Microbiol. 2009; 9: 159.

Raja AF, Ali F, Khan IA, Shawl AS, Arora DS. Acetyl-11-keto-β-boswellic acid (AKBA); targeting oral cavity pathogens. BMC Res Notes. 2011; 4: 406.

Setiawati S, Nuryastuti T, Ngatidjan N, Mustofa M, Jumina J, Fitriastuti D. In vitro antifungal activity of (1)-N-2-Methoxybenzyl-1, 10-phenanthrolinium Bromide against Candida albicans and its effects on membrane integrity. Mycobiology. 2017; 45: 25–30.

Fujibayashi T, Nakamura M, Tominaga A, Satoh N, Kawarai T, Narisawa N, Shinozuka O, Watanabe H, Yamazaki T, Senpuku H. Effects of IgY against Candida albicans and Candida spp. adherence and biofilm formation. Jpn J Infect Dis. 2009; 62(5): 337–42.

Brezski RJ, Jordan RE. Cleavage of IgGs by proteases associated with invasive diseases: an evasion tactic against host immunity? MAbs. 2010; 2(3): 212–20.

Amerongen AN, Verman ECI, Abyono R. Ludah dan kelenjar ludah: arti bagi kesehatan gigi. Yogyakarta: Gadjah Mada University Press; 1991. p. 269.

Huang R, Li M, Gregory RL. Bacterial interactions in dental biofilm. Virulence. 2011; 2(5): 435–44.

Jakubovics NS. Intermicrobial interactions as a driver for community composition and stratification of oral biofilms. J Mol Biol. 2015; 427(23): 3662–75.


Refbacks

  • There are currently no refbacks.


View My Stats