Effects of soy isoflavone genistein on orthodontic tooth movement in guinea pigs

Sri Suparwitri, Pinandi Sri Pudyani, Sofia Mubarika Haryana, Dewi Agustina

= http://dx.doi.org/10.20473/j.djmkg.v49.i3.p168-174
Abstract views = 586 times | downloads = 502 times

Abstract


Background: Osteoblast and osteoclast are the important factor in periodontal tissue remodeling for the orthodontic treatment success. Resorption process takes place in compression area by osteoclast and apposition in the tension area by osteoblast. In general hormone condition and age affect remodeling process. Estrogen has a high contribution in remodelling process and decreased in elderly individual such as menopausal women. Soybean contains isoflavone genistein which has similar structure and activity to estrogen. Many researchers indicate that isoflavone genistein not only has an inhibitor effect in osteoporosis but also has estrogenic and antiestrogenic effect as well. Purpose: The study aimed to investigate the effect of soybean isoflavone genistein administration on osteoblast and osteoclast cells number in orthodontic tooth movement of young and old guinea pigs. Method: The research was quasi-experimental study with post test only with control design. The experimental animals were 24 male guinea pigs that divided into: young guinea pigs (±4 months old) and old guinea pigs (±2.5 years old). Each group was divided into 4 subgroups for receiving the treatment namely; control, orthodontic treatment, genistein treatment and orthodontic+genistein treatment. All of the subjects were sacrificed at day 7 and the specimens were histologically analyzed using tartrate resistance acid phosphatase (TRAP) and hematoxylin eosin (HE) staining and observed using microscope that connected to obtilab and an image raster program. Result: U Mann-Whitney statistical analysis showed there were significant differences in osteoblast cell numbers; between orthodontic treatment and orthodontic+genistein treatment in the old guinea pigs (p=0.004); between orthodontic treatment in the young guinea pig and orthodontic+genistein treatment in the old guinea pig (p=0.016); between orthodontics treatment and orthodontic+genistein treatment in the young guinea pigs (p=0.025). U Mann-Whitney statistical analysis showed there were significant differences in osteoclast cell numbers: between the orthodontic treatment in the old guinea pig and orthodontics+genistein treatment in the young guinea pigs (p=0.007); between orthodontic treatment group in the young guinea pigs and orthodontics+genistein treatment in the old guinea pigs; between orthodontic treatment and orthodontic+ genistein treatment in the young guinea pigs (p=0.007). All groups administered by genistein the numbers of osteoblast in the surrounding of the tension sites increased, while in the surrounding of the compression sites had less osteoclasts; even, there were no osteoclasts found in some samples. Conclusion: Soybean isoflavone genistein administration on orthodontic tooth movement increased osteoblast numbers in the tension sides and decreased osteoclast numbers in the compression sides.

Keywords


isoflavone genistein of soybean; guinea pig; orthodontic tooth movement

Full Text:

PDF

References


Dewanto H. Aspek-aspek epidemiologi maloklusi. Yogyakarta: Gadjah Mada University Press; 1993. p. 1-77.

Graber RL, Vanarsdall Jr. Orthodontics, current principles and techniques. 4th ed. Mosby, St. Louis: Vig Publisher; 2000. p. 557-646.

English JD, Buschang PH, Throckmorton GS. Does malocclusion affect masticatory performance?. Angle Orthod 2002; 72(1): 21-7.

Dewi O. Analisis hubungan maloklusi dengan kualitas hidup pada remaja SMU Kota Medan Tahun 2007. Tesis. Medan: Universitas Sumatera Utara; 2008.

Atashi MHA. Prevalence of malocclusion in 13-15 year-old adolescents in Tabriz. JODDD 2007; 1(1): 1-6.

Laguhi VA, Anindita PS, Gunawan PN. Gambaran maloklusi dengan menggunakan HMAR pada pasien di rumah sakit gigi dan mulut Universitas Sam Ratulangi Manado. Jurnal e-Gigi 2014; 2(2): 1-7.

Lew KK, Foong WC, Loh E. Malocclusion prevalence in an ethnic Chinese population. Aust Dent J 1993; 38(5): 442-9.

Roberts-Harry D, Sandy J. Orthodontics. Part 10: Impacted teeth. Br Dent J 2004; 196: 319-27.

Ren Y, Maltha JC, Van‘t Hof MA, Kuijpers-Jagtman, AM. Age effect on orthodontic tooth movement in rats. J Dent Res 2003; 82(1): 38-42.

Mundy GR. Nutritional modulators of bone remodelling during aging. Am J Clin Nutr 2006; 83(suppl): 427S-30S.

Parfitt AM. Bone remodeling. Relationship to the amount and structure of bone and pathogenesis and prevention of fractures. In: Riggs BL, Melton LJ, editors. Osteoporosis etiology, diagnosis and management. New York: Raven Press; 1988.

Ma DF, Qin LQ, Wang PY, Katoh R. Soy isoflavone intake inhibits bone resorption and stimulates bone formation in menopausal women: meta-analysis of randomized controlled trials. Eur J Clin Nutr 2008; 62(2): 155-61.

Cornwell T, Cohick W, Raskin I. Dietary phytoestrogens and health. Phytochemistry 2004; 65(99): 5-1016.

Kini U, Nandeesh BN. Physiology of bone formation, remodeling, and metabolism. In: Fogelman I, Gnanasegaran G, Van der Wall H, editors. Radionuclide and hybrid bone imaging. Springer-Verlag Berlin Heidelberg; 2012. p. 29-55.

Ariffin, SHZ, Yamamoto, Abidin lZZ, Wahab RMA, Ariffin ZZ. Cellular and molecular changes in orthodontic tooth movement, Review Article. Scientific World J 2011; 11: 1788–803.

Setchell KDR, Adlercreutz H. Mammalian lignans and phytoestrogens. Recent studies on their formation, metabolism and biological role in health and disease. In: Rowland IR, editor. Role of the gut flora in toxicity and cancer. London, UK: Academic Press; 1988. p. 315-45.

Tepavčević V, Cvejić J, Poša M, Popović J. Isoflavone content and composition in soybean. In: Tzi Bun Ng, editor. Soybeanbiochemistry, chemistry, and physiology. Croatia: InTech; 2011. p. 281-94.

Price K, Fenwick G. Naturally occurring oestrogens in foods-A review. Food Addit Contam 1985; 2: 73 -106.

Turhan NO, Bolkan F, Duvan CI, Ardicoglu Y. The effect of isoflavones on bone mass and bone remodelling markers in postmenopausal women. Turk J Med Sci 2008; 38(2): 145-52.

Kalajzic Z, Peluso EB, Utreja A, Dyment N, Nihara J, Xu M, Chen J, Uribe F, Wadhwa S. Effect of cyclical forces on the periodontal ligament and alveolar bone remodeling during orthodontic tooth movement. Angle Orthod 2014; 84: 297-303.

MirHashemi AH, Afshari M, Alaeddini M, Etemad-Moghadam S, Dehpour A, Sheikhzade S, Akhoundi MSA. Effect of Atorvastatin on orthodontic tooth movement in male wistar rats. J Dent (Tehran) 2013; 10(6): 532-9.

Dang ZC, Audinot V, Papapoulos SE, Boutin JA, Lowik CWGM. Peroxisome proliferator-activated receptor γ (PPARγ) as a molecular target for the soy phytoestrogen genistein. J Biol Chem 2003; 278(2): 962-7.

Dang ZC, Lowik C. Dose-dependent effects of phytoestrogens on bone. Trends Endocrinol Metab 2005; 25(5): 208-13.

Tsuda M, Kitazaki T, Ito T, Fujita T. The effect of ipriflavone (TC-80) on bone resorption in tissue culture. J Bone Miner Res 1986; 1: 207-11.

Ushiroyama T, Okamura S, Ikeda A, Ueki M. Efficacy of ipriflavone and 1 alpha vitamin D therapy for the cession of vertebral bone loss. Int J Gynaecol Obstet 1995; 48: 283-8.

Kuiper GJM, Lemmen JG, Carlsson BO, Corton JC, Safe SH, Van Der Saag PT, Van Der Burg B, Gudtafsson J-A. Interaction of estrogenic chemicals and phytoestrogen with estrogen receptor β. Endocrinology 1998; 139(10): 4252-62.

Yamaguchi M. Isoflavone and bone metabolism: its cellular mechanism and preventive role in bone loss. J Health Sci 2002; 48(3): 209-22.


Refbacks

  • There are currently no refbacks.


View My Stats