Effects of Moringa oleifera leaf extract combined with DFBBX on type-1 collagen expressed by osteoblasts in the tooth extraction sockets of Cavia cobaya

S. Soekobagiono; Sherman Salim; Hanoem Eka Hidayati; Karina Mundiratri

doi:10.20473/j.djmkg.v51.i2.p86-90

Effects of Moringa oleifera leaf extract combined with DFBBX on type-1 collagen expressed by osteoblasts in the tooth extraction sockets of Cavia cobaya

S. Soekobagiono, Sherman Salim, Hanoem Eka Hidayati, Karina Mundiratri

= http://dx.doi.org/10.20473/j.djmkg.v51.i2.p86-90

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Abstract

Background: Tooth extraction is a common procedure in dentistry after which the residual ridge will no longer receive stimulus leading to volume, height and width loss. These anatomical changes can then result in difficulties with future denture fabrication and implant placement. Preservation of the alveolar ridge, therefore, assumes considerable importance after tooth extraction. Moringa oleifera, on the other hand, can enhance bone formation. Type-1 collagen is a marker of osteoblast formation. Purpose: This research aimed to analyze the effects of Moringa oleifera leaf extract combined with DFBBX on type-1 collagen expressions in tooth extraction sockets. Methods: 56 Cavia cobaya subjects were divided into eight groups. Their lower left incisors were then extracted prior to the sockets of the first and fifth groups being filled with PEG, those of the second and sixth groups with DFBBX, those of the third and seventh groups with Moringa oleifera leaf extract and a combination of DFBBX and Moringa oleifera leaf extract in those of the fourth and eighth groups. The sockets were then examined on days 7 and 30 by means of an immunohistochemical technique. The data collected was subsequently subjected to analysis by One Way Anova and Tukey HSD tests. Results: There were significant differences between the control group and the treatment group administrated with Moringa oleifera leaf extract combined with DFBBX. On days 7 and 30, the groups treated with the combination of DFBBX and Moringa oleifera leaf extract had the highest number of type-1 collagen expressions. Conclusion: A combination of DFBBX and Moringa oleifera leaf extract is effective in increasing type-1 collagen expressions in tooth extraction sockets.

Keywords

alveolar bone; DFBBX; Moringa oleifera leaf extract; type-1 collagen; socket preservation

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References

Mezzomo LA, Shinkai RS, Mardas N, Donos N. Alveolar ridge preservation after dental extraction and before implant placement: a literature review. Rev Odonto Ciência. 2011; 26(1): 77–83.

Van Der Weijden F, Dell’Acqua F, Slot DE. Alveolar bone dimensional changes of post-extraction sockets in humans: a systematic review. J Clin Periodontol. 2009; 36(12): 1048–58.

Salmen FS, Oliveira MR, Gabrielli MA, Piveta ACG, Filho VAP, Gabrielli MFR. Bone grafting for alveolar ridge reconstruction. Review of 166 cases. Rev Col Bras Cir. 2017; 44(1): 33–40.

Murphy CM, O’Brien FJ, Little DG, Schindeler A. Cell-scaffold interactions in the bone tissue engineering triad. Eur Cells Mater. 2013; 26: 120–32.

Kamadjaja DB, Harijadi A, Soesilawati P, Wahyuni E, Maulidah N, Fauzi A, Rah Ayu F, Simanjuntak R, Soesanto R, Asmara D, Rizqiawan A, Agus P, Pramono C. Demineralized freeze-dried bovine cortical bone: its potential for guided bone regeneration membrane. Int J Dent. 2017; 2017: 1–10.

Coppin J. A study of the nutritional and medicinal values of Moringa oleifera leaves from sub-Saharan Africa: Ghana, Rwanda, Senegal and Zambia. Thesis. New Jersey: The State University of New Jersey; 2008. p. 5-99.

Patel C, Rangrez A, Parikh P. The anti-osteoporotic effect of Moringa oliefera on osteoblastic cells : SaOS 2. IOSR J Pharm Biol Sci. 2013; 5(2): 10–7.

Bakar A. Kedokteran gigi klinis. 2nd ed. Yogyakarta: Quantum Sinergis Media; 2012. p. 117.

Pudianto A. Dosis efektif kombinasi ekstrak daun kelor (Moringa oliefera) dengan bone graft pada soket pencabutan gigi terhadap osteoblas dan osteoklas tulang alveolar Cavia cobaya. Thesis. Surabaya: Universitas Airlangga; 2015. p. 50-72.

Wirawan S. Pemberian ekstrak daun kelor (Moringa oliefera) dan bone graft serta kombinasi keduanya pada soket pencabutan gigi terhadap pembentukan sel osteoblas dan sel osteoklas (Cavia cobaya). Thesis. Surabaya: Universitas Airlangga; 2015. p. 30-50.

Lu Y, Kamel-El Sayed SA, Wang K, Tiede-Lewis LM, Grillo MA, Veno PA, Dusevich V, Phillips CL, Bonewald LF, Dallas SL. Live imaging of type I collagen assembly dynamics in osteoblasts stably expressing GFP and mCherry-tagged collagen constructs. J Bone Miner Res. 2018; 33(6): 1166–82.

Al-Ghamdi H, Mokeem SA, Anil S. Current concepts in alveolar bone augmentation : a critical appraisal. Saudi Dent J. 2007; 19(2): 74–90.

Gupta R, Pandit N, Malik R, Sood S. Clinical and radiological evaluation of an osseous xenograft for the treatment of infrabony defects. J Can Dent Assoc. 2007; 73(6): 513–513f.

Tan WS, Arulselvan P, Karthivashan G, Fakurazi S. Moringa oleifera flower extract suppresses the activation of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 macrophages via NF-κB pathway. Mediators Inflamm. 2015; 2015: 1–11.

Karthivashan G, Tangestani Fard M, Arulselvan P, Abas F, Fakurazi S. Identification of bioactive candidate compounds responsible for oxidative challenge from hydro-ethanolic extract of Moringa oleifera leaves. J Food Sci. 2013; 78(9): C1368–75.

Schett G. Effects of inflammatory and anti-inflammatory cytokines on the bone. Eur J Clin Invest. 2011; 41(12): 1361–6.

Park HJ, Baek K, Baek JH, Kim HR. TNFα increases RANKL expression via PGE2-induced activation of NFATc1. Int J Mol Sci. 2017; 18(3): 1–15.

Burali SC, Kangralkar V, Sravani OS, Patil SL. The beneficial effect of ethanolic extract of Moringa oleifera on osteoporosis. Int J Phamaccutical Appl. 2010; 1: 50–8.

Patisaul HB, Jefferson W. The pros and cons of phytoestrogens. Front Neuroendocr. 2010; 31(4): 400–19.

Schilling T, Ebert R, Raaijmakers N, Schütze N, Jakob F. Effects of phytoestrogens and other plant-derived compounds on mesenchymal stem cells, bone maintenance and regeneration. J Steroid Biochem Mol Biol. 2014; 139: 252–61.

Barrett K, Brooks H, Boitano S, Barman S. Ganong’s review of medical physiology. 23rd ed. New York: McGraw Hill Medical; 2010. p. 261-72.

Henriksen K, Karsdal MA. Type I collagen. In: Karsdal MA, editor. Biochemistry of collagens, laminins and elastin. Amsterdam: Academic Press; 2016. p. 1–11.

Guskuma MH, Hochuli-Vieira E, Pereira FP, Rangel-Garcia I, Okamoto R, Okamoto T, Filho OM. Evaluation of the presence of VEGF, BMP2 and CBFA1 proteins in autogenous bone graft: histometric and immunohistochemical analysis. J CranioMaxillofacial Surg. 2014; 42(4): 333–9.

Tomlin EM, Nelson SJ, Rossmann JA. Ridge preservation for implant therapy: a review of the literature. Open Dent J. 2014; 8: 66–76.

Kresnoadi U, Rahayu RP, Rubianto M, Sudarmo SM, Budi HS. TLR2 signaling pathway in alveolar bone osteogenesis induced by Aloe vera and xenograft (XCB). Braz Dent J. 2017; 28(3): 281–6.

Kresnoadi U, Ariani MD, Djulaeha E, Hendrijantini N. The potential of mangosteen (Garcinia mangostana) peel extract, combined with demineralized freeze-dried bovine bone xenograft, to reduce ridge resorption and alveolar bone regeneration in preserving the tooth extraction socket. J Indian Prosthodont Soc. 2017; 17(3): 282–8.

Rostiny R, Djulaeha E, Hendrijantini N, Pudijanto A. The effect of combined Moringa oleifera and demineralized freeze-dried bovine bone xenograft on the amount of osteoblast and osteoclast in the healing of tooth extraction socket of Cavia cobaya. Dent J (Maj Ked Gigi). 2016; 49(1): 37–42.