Application of Fish Probiotics in Catfish Farming Activities Using Biofloc System: Low and High Protein Feed Induction

biofloc feed protein sangkuriang catfish bacteria indigenous

Authors

  • Salsabila Dewi Maharani Aquatic Resource Management, Faculty of agriculture, University of Trunojoyo Madura, Indonesia
  • Ananda Wahyuni Aquatic Resource Management, Faculty of agriculture, University of Trunojoyo Madura, Indonesia
  • Abdus Salam Junaedi
    abdus.salamj@trunojoyo.ac.id
    Aquatic Resource Management, Faculty of agriculture, University of Trunojoyo Madura, Indonesia
  • Haryo Triajie Aquatic Resource Management, Faculty of agriculture, University of Trunojoyo Madura, Indonesia
  • Muhammad Zainuri Aquatic Resource Management, Faculty of agriculture, University of Trunojoyo Madura, Indonesia
September 30, 2025

Catfish farming with the biofloc system is increasingly used because it can increase feed efficiency. However, the main challenge in this system is optimal water quality, especially in relation to the presence of pathogenic bacteria such as coliform. This study aims to determine the effect of giving probiotics to feed with high, low, and control protein content on water quality, water and intestinal TPC, and the level of coliform bacteria in the anus of catfish. The analysis method used is pour plate and spread plate. In addition, water quality such as pH, temperature, was also observed to determine the relationship between environmental conditions and bacterial development. The main objective of this study was to determine the differences in water quality values, TPC in water and intestines, and coliform bacteria levels in each treatment. The results of this study are expected to provide a better understanding of how the use of probiotics. Temperature values ​​ranged from 26.71-28.83°C and pH ranged from 8.34-9.45. Bacterial TPC values ​​ranged from 8.03-16.94 CFU/ml. The TC value of bacteria in catfish anus samples ranged from 3.81-7.00 CFU/ml. This study can be a practical solution for farmers to improve sustainable catfish cultivation.