IN VITRO FERTILITY TEST OF HUMAN SPERMATOZOA MEMBRANE PROTEIN FERTILIN BETA ANTIBODY IN MICE (Mus musculus Balb/c) AS IMMUNOCONTRACEPTIVE CANDIDATE

Reny I’tishom, Doddy M Soebadi, Aucky Hinting, Hamdani Lunardhi, Rina Yudiwati

= http://dx.doi.org/10.20473/fmi.v52i3.5453
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Abstract


One of the materials as potential candidates immunocontraception material is spermatozoa. Fertilin beta is spermatozoa membrane protein and is found only in mature spermatozoa and ejaculate, which serves as an adhesion molecule. Spermatozoa membrane protein that is used as an ingredient immunocontraception candidate, must have specific criteria that the specificity of spermatozoa, the role of antigen in the fertilization process, which includes the formation of immunogenicity sufficient antibody response has the potential to block fertilization. Antibodies against spermatozoa affect the stages before fertilization of the reproductive process and can hinder the development of the embryo after fertilization. Until now very little research data spermatozoa membrane protein as an ingredient immunocontraception are up to the test of experimental animals. The research objective is to prove the role of the resulting antibody induction of antibodies fertilin beta protein in the membrane of human spermatozoa induce agglutination and reduce motility thus reducing the number of in vitro fertilization. Research conducted at the IVF Laboratory, Department of Biology of Medicine, Faculty of Medicine, University of Airlangga. This research includes: Test the potential of antibody protein beta fertilin membrane of human spermatozoa and inhibit the role of antibodies in vitro fertilization in mice (Mus musculus Balb/c). In vitro studies have resulted in fertilization figure of 25% is smaller than the number that is equal to control fertilization of 58.7%, whereas previously the spermatozoa were incubated first with a beta membrane protein antibody fertilin human spermatozoa. While the percentage of inhibition of sperm to fertilize an oocyte by 33.75%. Potential imunokontraseptif considered effective if it decreased significantly (P <0.05) than the numbers fertilization in the treatment group compared with the control group. This shows fertilin beta membrane protein antibody has the ability to inhibit human spermatozoa to fertilize oocytes that reduce the number of fertilization.

Keywords


beta membrane protein antibody fertilin human spermatozoa; fertilization invitro; Mus musculus Balb/c

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References


Bohring C, Krause E, Habermann B, Krause W (2001). Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease. Mol Hum Reprod 7, 113-118

Calamera JC, Doncel GF, Brugo-Olmedo S, Sayago A, Acosta AA (2002). Male antisperm antib-odies:association with a modified sperm stress test and lipid peroxidation. Andrologia 34, 63-68

Darsini N, Lunardhi H, Aswin RH, I’tishom R, Hinting A (2012). Synthesis of beta fertilin protein polyclonal antibody of human sperm membrane as a candidat for immunocontraceptive material. Folia Medica Indonesiana 48, 6-11

Domagala A and Kurpisz M (2004). Identification of sperm immunoreactive antigen for immunocontra-ceptive purposes: a review. Reproductive Biology and Endocrinology 2, 11-18

Elder K and Dale B (2000). In vitro fertilization, 2nd ed, United Kingdom, Cambridge University Press

Faizah Z (2013). Perbandingan maturasi, morfologi dan fertilisasi antara oosit sapi yang divitrifikasi pra dan pasca maturasi invitro. Thesis. Universitas Airlangga, Surabaya

Griffin PD (2003). Contraceptive Vaccines. Available from http://www.gfmer.ch/Books/Reproductive_ health/Contraceptives_vaccines.html. Accessed November 2, 2015

I’tishom R, Soebadi DM, Hinting A, Lestari U (2013). Characterization of fertilin beta protein of human sperm a candidate for immunocontracetion. Folia Medica Indonesiana 49, 26-32

Martinez ML and Harris JD (2000). Effectiveness of zona pellucida protein ZPB as an immunocontraceptive antigen. Journal of Reproduction and Fetility 120, 19-32

Mayonda IT (2006). The Immunology of Human Reproduction, London, Taylor & Francis Group

Naz RK (1996). Application of sperm antigen in immunocontraception. Frontier in Bioscience 1, e87-95

Naz RK and Zhu X (1998). Recombinant fertilization antigen-1 causes a contraceptive effect in actively immunized mice. Biol Reprod 59, 1095-1100

Naz RK, Gupta SK, Gupta JC, Vyas HK, Talwar GP (2005). Recent advances in contraceptive vaccine development: a mini-review. Human Reproduction 20, 3271-3283

Naz RK (2006). Antisperm immunity for contraception. Journal of Andrology 27, 153-159

Ramalho-Santos J, Ricardo DM, Sutovsky P, Wing-Sang Chan A, Hewitson L, Gary MW, Simerly CR, Schatten G (2000). SNAREs in mammalian sperm: possible implications for fertilization. Developmental Biology 223, 54-69

Wahyuningsih SPA (2011). Analisis potensi protein subunit membran spermatozoa kelinci sebagai kandidat bahan immunocontraception. Dissertation. Universitas Airlangga, Surabaya

Widjiati, Sukra Y, Purwantara B, Djuwita I (2011). The role of glucose and phosphate in vitro culture medium to overcome cell block on mouse embryo. Folia Medica Indonesiana 47, 166-170


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