CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL

Prihartini Widiyanti; Bambang Prajogo; Ni Putu Ermi Hikmawati

doi:10.20473/ijtid.v6i1.1207

CYTOTOXICITY OF JUSTICIA GENDARUSSA BURM F. LEAF EXTRACTS ON MOLT-4 CELL

Prihartini Widiyanti, Bambang Prajogo, Ni Putu Ermi Hikmawati

= http://dx.doi.org/10.20473/ijtid.v6i1.1207

Abstract views = 1833 times |

downloads = 919 times

Abstract

Justicia gendarussa Burm f. (Acanthaceae) is known for its activity as a male contraceptive and anti-HIV properties. The present study was designed to evaluate extracts of J. gendarussa for cytotoxicity activity against MOLT-4 cells. The cytotoxic activity of the fractionated-extract and 70% ethanol extracts of J. gendarussa leaves on MOLT-4 cells were evaluated using a WST-1 assay. The treatment cells, control cells without treatment and control media were also tested in duplicate. The absorbance was measured at a wavelength of 450 nm using a microplate absorbance reader (Bio-Rad). The average absorbance measures formazan produced by viable cells that metabolize the WST-1 reagent. Then the data was analyzed with regression analysis Microsoft Excel 2007 program to determine the concentration with 50% cell viability (50% Cytotoxicity Concentration, CC50). The CC50 values of the fractionated-extract and 70% ethanol extract of J. gendarussa leaves were 94 μg/ml and 78 μg/ml, respectively. The cytotoxicity of fractionated-extract and 70% ethanol extract of J. gendarussa leaves were not significantly different (p > 0.05). It can be concluded that the fractionated-extract and 70% ethanol extract of J. gendarussa leaves are not toxic to MOLT-4 cells.

Keywords

cytotoxicity; Justicia gendarussa Burm.f; MOLT-4 cell; WST-1 assay, anti HIV

Full Text:

PDF

References

Ratnasooriya WD, Deraniyagala SA, Dehigaspitiya DC. Antinoceptive activity and toxicological study of aqueous leaf extract of Justicia gendarussa Burm f. in rats. Pharmacogn Mag, 3, 2007: 145–155.

Prajogo B, Guliet D, Queiroz F, Wolfernder J-L, Cholies N, Aucky H, Hostettmann K. Isolation of Male Antifertility Compound in n-Butanol Fraction of Justicia gendarussa Burm f. Leaves. Folia Medica Indonesiana, 45 (1) 2009: 28–31.

Prajogo B, Widiyanti P, and Riza H. Effect of Ethanolic Extract of Justicia gendarussa Burm f. Against Activity of Reverse Transcriptase HIV Enzyme In Vitro. Jurnal Bahan Alam Indonesia, 8 (6) 2014: 384–388.

Prajogo B, Ifadotunnikmah F, Febriyanti AP, Jusak N. Efek Fase Air Daun Gandarusa (Justicia gendarussa Burm.f) pada Fungsi Hati dan Fungsi Ginjal Kelinci Jantan (Uji Toksisitas Fase Air Daun Gandarusa Sebagai Bahan Kontrasepsi Pria). Veterinaria Medika, 1(3) 2008: 79–82.

Prajogo B. Autentik Tanaman Justicia gendarussa Burm f. Sebagai Bahan Baku Obat Kontrasepsi Pria. Surabaya: Airlangga University Press dengan LP3 UNAIR; 2014.

Ayob Z, Samad AA, Bohari SPM. Cytotoxicity Activities in Local Justicia gendarussa Crude Extracts against Human Cell Lines. Jurnal Teknologi, 64 (2) 2013: 45–52.

Ayob Z, Bohari SPM, Samad AA, Jamil S. Cytotoxicity Activities in against Breast Cancer Cell of Local Justicia gendarussa Crude Extracts. Evidance-Based Complementary and Alternative Medicine, 2014: 1–12.

Chakravarty AK, Dastiar PPG, and Pakrashi SC. Simple Aromatic Amines from Justicia gendarussa 13C NMR Spectra of the Bases and Their Analogues. Tetrahedron, 18(12) 1982: 1797–1802.

Kiren Y, Deguchi J, Hirasawa Y, Morita H, Prajogo, B.Justidrusamides A-D, new 2-aminobenzyl Alcohol Derivatives from Justicia gendarussa. Journal of Natural Medicines; 2014.

Veljkovic V, Mouscadet J-F, Veljkovic N, Glisic S, Debyser Z. Simple Criterion for Selection of Flavonoid Compounds with Anti-HIV Activity. Bioorganic and Medicinal Chemistry Letters, 17, 2007: 1226–1232.

Ko Y-J, Oh H-J, Ahn H-M, Kang H-J, Kim J-H, Ko, YH. Flavonoids as Potential Inhiibitors of Retroviral Enzymes. J. Korean Soc. Appl. Biol. Chem, 52 (4) 2009: 321–326.

Bolton JL, Trush MA, Penning TM, Dryhurst G, Monks TJ. Role of Quinones in Toxicology. Chem Res Toxicol, 13 2000: 135–160.

Inayat-Hussain SH, Winski SL, Ross D. Different Involvement of Caspase in Hydroquinone-induced Apoptosis in Human Leucemic HL-60 and Jurcat Cells. ToxicolAppliPharmacol, 175 2001: 95–103.

Morin D, Barthelemy S, Zini R, Labidalle S, Tillement, JP. Curcumin Induces the Mitochondrial Permeability Transition Pore by Membrane Protein Thiol Oxidation. FEBSLett, 495, 2005: 131–136.

Salvi M, Brunati AM, Clari G, Toninello A. Interaction of Genistein with the Mitochondrial Electron Transport Chain Results in the Opening of the Membrane Transition Pore. Biochim Biophys Acta,1556, 2005: 187–156.