THE EFFECT OF GENDARUSSIN A ISOLATES OF Justicia gendarussa Burm.f. LEAF IN REVERSE TRANSCRIPTASE IN HIBITION OF HIV TYPE I INVITRO
Screening has been done to a few extracts from the leaves Justicia gendarussa Burm.f to see the growth rate of the virus from the blood plasma of HIV patients at Dr Soetomo Hospital. It is known that J. gendarussa leaf extract inhibits HIV type 1 reverse transcriptase. In addition, its main content is gendarussin A, besides gendarussin B, JGF1, JGF2 and JGF3, which have just identified. At the beginning, extraction and fractionation were performed with 3 models that highlight the absolute methanol, 70% methanol and 70% ethanol with the release of alkaloids. Furthermore, samples of each fraction were incubated in plasma of HIV patients with a titer of 3.6 10^6 copies for 1 h in concentrations of 1.64 ppm, 4.1 ppm, 8.2 ppm, 16.4 ppm and 41.0 ppm. After incubation, examination was performed by using Nucli sens a machine, which is a combination of PCR and Elisa, thus avoiding direct contact with the highly pathogenic virus. The result showed that the activity sequence from the most potential to the weak, among others, was 1.64 ppm >4.1 ppm > 8.2 ppm > 16.4 ppm > 41.0 ppm, each with barriers value of 0.62 10^6, 1.4 10^6, 1.6 10^6, 2.4 10 cells/ml. In conclusion, highest anti-HIV activity comes from the concentration of gendarussin A isolate at 1.64 ppm. Furthermore, after linear
regression of y = -3.063 x + 81.37 was done, the IC50 of 10.24 ppm was obtained.
Health Profile: Indonesia. United States Agency for International Development (March 2008). Accessed August 25, 2008.
JoAnn O’Toole, Lauren Robertson, Susan Walters Schmid, https://www.atrainceu.com/course/washington-hiv-aids-4-units-136
Yu LM, Easterbrook PJ, Marshall T, 1997. Relationship between CD4 Countand CD4% in HIV infected people. International Jof Epidemiology, Vol 26 No 6, Great Britain.
Ng, TB, Huang B, Fong,WP., Yeung., H.W., 1997. Anti-human immunodeficiency virus(anti-HIV) natural products with special emphasis on HIV reverse trancriptase inhibitors. Life Science 61, 933-949.
Lee-Huang, S.,Huang, P.L., Nara, P.L, Chen, H.C., Kung, H.F., Huang, P., Huang, H.I,, Huang, P.L., 1990. MAP 30 A New inhibitor of HIV-1 infection and replication, FEBS Letters 272, 12-18.
Woradulanyapinij, W., Soonthornchareonnon, N., Wiwat, C., 2005. In vitro HIV type 1 reverse transcriptase inhibitory activities ofThai medicinal plants and Canna indica L. rhizomes. Journal of Ethnopharmacology 101, pp. 84-89.
Andrae-Marobela, Kerstin; Ghislain, Fotso Wabo; Okatch, Harriet; Majinda, Runner R. T, 2013. Polyphenols: A Diverse Class of Multi-Target Anti-HIV-1 Agents Current Drug Metabolism;May2013, Vol. 14 Issue 4, p392
Prajogo BEW, Noor CH, Hudi, Aucky H, Dian, Mustaina, Kasmijati, Anggraeni, Radjaram, 2008. Pengaruh ekstak etanol 70 % pada pria fertile (Uji klinik fase I). Fakultas Farmasi Unair-BKKBN Pusat.
Prajogo BEW, Noor CH, Hudi, Aucky H, Dian, Mustaina, Flouresia, Anggraeni, Radjaram, 2009. Pengaruh ekstak etanol 70 % pada pria pasangan usia subur (PUS) (Uji klinik fase II). Fakulas Farmasi Unair-BKKBN Pusat.
Prajogo BEW, Nasronudin, Noor CH dan Neny P, 2009. Aktivitas penghambatan Reverse Transcriptase HIV tipe I tanaman obat Justicia gendarussa Burm.f. LPPM Unair.
Izzah Z, Prajogo BEW, Radjaram A, 2010. Studi Stabilitas Kimia Gendarussin A dalam sediaan Granul Fraksi Air Daun Justicia Gendarussa Burm F, Majalah Farmasi Airlangga, Vol.8 No.1, April 2010
Nianhang Chen, Lian Wen, Henry Lau, Sekhar Surapaneni, and Gondi Kumar, 2012. Pharmacokinetics, metabolism and excretion of [14C]-lenalidomide following oral administration in healthy male subjects Cancer Chemother Pharmacol. 2012 Mar; 69(3): 789–797. Published online 2011 Oct 29. doi: 10.1007/s00280-011-1760-3CID: PMC3286592
The Indonesian Journal of Tropical and Infectious Disease (IJTID) is a scientific peer-reviewed journal freely available to be accessed, downloaded, and used for research. All articles published in the IJTID are licensed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which is under the following terms:
Attribution — You must give appropriate credit, link to the license, and indicate if changes were made. You may do so reasonably, but not in any way that suggests the licensor endorses you or your use.
NonCommercial — You may not use the material for commercial purposes.
ShareAlike — If you remix, transform, or build upon the material, you must distribute your contributions under the same license as the original.
No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.