Teguh Hari Sucipto, Siti Churrotin, Harsasi Setyawati Setyawati, Kris Cahyo Mulyatno, Ilham Harlan Amarullah, Shuhai Ueda, Tomohiro Kotaki, Sri Sumarsih, Puspa Wardhani, Sri Subekti Bendryman, Aryati Aryati, Soegeng Soegijanto, Masanori Kameoka

Abstract views = 1087 times | downloads = 509 times


Dengue virus (DENV) is a significant pathogen emerging worldwide as a cause of infectious disease. Antidengue treatments are urgently required to control the emergence of dengue. DENV is a mosquito-borne disease responsible for acute systemic diseases and serious health conditions. DENVs were distributed in the tropical and sub-tropical areas and transmitted to humans by Aedes agypty and Aedes albopictus. Dengue vaccine or antiviral has not yet been clinically approved for humans, even though there have been great efforts toward this end. Antiviral activity against DENV is an important alternative for the characterization and development of drugs. Metal–organic compounds were reported to exhibit fungicidal, bactericidal, and antiviral activities its inhibitory activity was not significant, at high concentration it was more toxic to replicating cells than to stationary cell monolayers of Vero cells. The aim of this study is to investigate the antiviral effects of Cobalt(II)–Morin complex. This compound was further investigated for its inhibitory effect on the replication of DENV-2 in Vero cells. The replication of DENV was measured by enzyme-linked immunosorbent assay and the value of selectivity index (SI). SI was determined as the ratio of the 50% cytotoxic concentration (CC50) to the 50% inhibitory concentration (IC50). The IC50 value of the Cobalt(II)–Morin complex for DENV-2 was 3.08 µg/ml, and the CC50 value of the complex for Vero cells was 3.36 µg/ml; thus, the SI value was 1.09. The results of this study demonstrate the antidengue serotype 2 inhibitory activity of Cobalt(II)–Morin complex and its high toxicity in Vero cells. Further studies are not required before Co(II)–Morin can be applied in the treatment of DENV-2 infections.


cobalt(II); morin; complex compound; inhibitory activity; DENV-2

Full Text:



Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature. 2013 Apr 7;496(7446):504–7.

Woodland DL. Vaccines Against Dengue Virus. Viral Immunol. 2015 Mar;28(2):75–75.

Lim SP, Noble CG, Shi P-Y. The dengue virus NS5 protein as a target for drug discovery. Antiviral Res. 2015 Jul;119:57–67.

Assunção-Miranda I, Cruz-Oliveira C, Neris RLS, Figueiredo CM, Pereira LPS, Rodrigues D, et al. Inactivation of Dengue and Yellow Fever viruses by heme, cobalt-protoporphyrin IX and tin-protoporphyrin IX. J Appl Microbiol. 2016 Mar;120(3):790–804.

ARIMA H, ASHIDA H, DANNO G. Rutin-enhanced Antibacterial Activities of Flavonoids against Bacillus cereus and Salmonella enteritidis. Biosci Biotechnol Biochem. 2002 Jan 22;66(5):1009–14.

Subash S, Subramanian P. Morin a flavonoid exerts antioxidant potential in chronic hyperammonemic rats: A biochemical and histopathological study. Mol Cell Biochem. 2009;327(1–2):153–61.

Fang S-H, Hou Y-C, Chang W-C, Hsiu S-L, Lee Chao P-D, Chiang B-L. Morin sulfates/glucuronides exert anti-inflammatory activity on activated macrophages and decreased the incidence of septic shock. Life Sci. 2003 Dec;74(6):743–56.

Gravina HD, Tafuri NF, Silva Júnior A, Fietto JLR, Oliveira TT, Diaz MAN, et al. In vitro assessment of the antiviral potential of trans-cinnamic acid, quercetin and morin against equid herpesvirus 1. Res Vet Sci. 2011 Dec;91(3):e158–62.

Merchant B. Gold, the Noble Metal and the Paradoxes of its Toxicology. Biologicals. 1998 Mar;26(1):49–59.

Nagender Reddy Panyala, Eladia María Peña-Méndez, Josef Havel. Gold and nano-gold in medicine: overview, toxicology and perspectives. J Appl Biomed. 2009;7(May):75–91.

Mao W, Bao K, Feng Y, Wang Q, Li J, Fan Z. Synthesis, crystal structure, and fungicidal activity of triorganotin(IV) 1-methyl-1H-imidazole-4-carboxylates. Main Gr Met Chem. 2015 Jan 1;38(1–2).

Lu X, Ye J, Zhang D, Xie R, Bogale RF, Sun Y, et al. Silver carboxylate metal–organic frameworks with highly antibacterial activity and biocompatibility. J Inorg Biochem. 2014 Sep;138:114–21.

Chang EL, Simmers C, Knight DA. Cobalt Complexes as Antiviral and Antibacterial Agents. Pharmaceuticals. 2010 May 26;3(6):1711–28.

Aguado S, Quirós J, Canivet J, Farrusseng D, Boltes K, Rosal R. Antimicrobial activity of cobalt imidazolate metal–organic frameworks. Chemosphere. 2014 Oct;113:188–92.


Smee DF, Bray M, Huggins JW. Antiviral activity and mode of action studies of ribavirin and mycophenolic acid against orthopoxviruses in vitro. Antivir Chem Chemother. 2001;12(6):327–35.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods. 1983 Dec;65(1–2):55–63.

Sucipto TH, Churrotin S, Setyawati H, Kotaki T, Martak F, Soegijanto S. ANTIVIRAL ACTIVITY OF COPPER(II)CHLORIDE DIHYDRATE AGAINST DENGUE VIRUS TYPE-2 IN VERO CELL. Indones J Trop Infect Dis. 2017 Aug 22;6(4):84.

Carvalho OV, Botelho CV, Ferreira CGT, Ferreira HCC, Santos MR, Diaz MAN, et al. In vitro inhibition of canine distemper virus by flavonoids and phenolic acids: Implications of structural differences for antiviral design. Res Vet Sci. 2013 Oct;95(2):717–24.

Lv J, Liu T, Cai S, Wang X, Liu L, Wang Y. Synthesis, structure and biological activity of cobalt(II) and copper(II) complexes of valine-derived schiff bases. J Inorg Biochem. 2006 Nov;100(11):1888–96.


  • There are currently no refbacks.

Copyright (c) 2017 Indonesian Journal of Tropical and Infectious Disease

View My Stats

IJTID Indexed by : 


IJTID (pISSN 2085-1103eISSN 2356-0991is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Lembaga Penyakit Tropis (Institute of Tropical Disease)

Universitas Airlangga

Kampus C Mulyorejo

Surabaya 60115