Effect of electrolyzed reduced water on Wistar rats with chronic periodontitis on malondialdehyde levels

Rini Devijanti Ridwan, Wisnu Setyari Juliastuti, R. Darmawan Setijanto

DOI: http://dx.doi.org/10.20473/j.djmkg.v50.i1.p10-13
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Abstract


Background: Periodontal disease is a progressive destructive change that causes loss of bone and periodontal ligaments around the teeth that can eventually lead to its loss. The main bacteria in chronic periodontitis is Porphyromonas gingivalis. Aggregatibacter actinomycetemcomitans, a pathogen associated with aggressive periodontitis, initiates a proinflammatory response that causes tissue destruction of periodontal, alveolar bone resorption and subsequent tooth loss. Electrolyzed reduced water (ERW) is an alkaline water, ERW not only has a high pH and low oxidation reduction potential (ORP), but also contains several magnesium ions. Magnesium ions proven effective for the prevention of various diseases. Purpose: To analyze the level of malondialdehyde (MDA) in Wistar rats with cases of chronic and aggressive periodontitis that consumed ERW. Method: Wistar rats were divided into four groups, each group with 10 rats. The first and second group were Wistar rat with chronic periodontitis and consume drinking water and ERW. The third and fourth group were Wistar rat with aggressive periodontitis and consume drinking water and ERW. This experiment is done by calculating the levels of MDA. The calculation of the levels of MDA is done with spectrophotometric assay for MDA. Result: The results of this experiment show that the level of MDA in serum in group that consume ERW had decreased significantly different with thegroup that consume drinking water with the statistical test. Conclusion: It can be concluded that ERW can decrease the MDA level in Wistar rat with chronic and aggressive periodontitis case.

Keywords


chronic periodontitis; aggressive periodontitis; electrolyzed reduced water; malondialdehyde level; Wistar rat

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References


Park SK, Park SK. Electrolyzed-reduced water increases resistance to oxidative stress, fertility, and lifespan via insulin/IGF-1-like signal in C. elegans. Biol Res 2013; 46(2): 147-52.

Shirahata S, Hamasaki T, Teruya K. Advanced research on the health benefit of reduced water. Trends in Food Science & Technology 2012; 23: 124-31.

Kobayasi T, Kaneko S, Tahara T, Hayakawa M, Abiko Y, Yoshie H. Antibody responses to Porphyromonas gingivalis hemagglutinin A and outer membrane protein in chronic periodontitis. J Periodontol 2006; 77(3): 364-9.

American Academy of Periodontology. Parameter on aggressive periodontitis. Journal of Periodontol 2000; 71(5 Suppl): 867–9.

Demmer RT, Papapanou PN. Epidemiologic patterns of chronic and aggressive periodontitis,. Periodontology 2000, 2010; 53(1): 28–44.

Armitage GC, Cullinan MP. Comparison of the clinical features of chronic and aggressive periodontitis. Periodontol 2000, 2010; 53: 28-44.

Hendler A, Mulli TK, Hughes FJ, Perrett D, Bombardieri M, HouriHaddad Y, Weiss EI, Nissim A. Involvement of autoimmunity in the pathogenesis of aggressive periodontitis. J Dent Res 2010; 89(12): 1389-94

Zainuri M, Wanandi SI. Aktivitas spesifik manganese superoxide dismutase (Mnsod) dan katalase pada hati tikus yang diinduksi hipoksia sistemik: hubungannya dengan kerusakan oksidatif. Media Litbang Kesehatan 2012; 22(2): 87-92

Bag A, Bag N. Target sequence polymorphism of human manganese superoxide dismutase gene and its association with cancer risk: a review. Cancer Epidemiol Biomarkers Prev 2008; 17(12): 3298-305.

Harju T, Kaarteenaho-Wiik R, Sirviö R, Pääkkö P, Crapo JD, Oury TD, Soini Y, Kinnula VL. Manganese superoxide dismutase is incresed in the airways of smokers’ lungs. Eur Respir J 2004; 24(5): 765-71.

Park SK, Kim JJ, Yu AR, Lee MY, Park SK. Electrolyzed reduced water confers increased resistance to environmental stresses. Mol Cell Toxicol 2012; 8(3): 241-7.

Ohsawa I, Ishikawa M, Takahashi K, Watanabe M, Nishimaki K, Yamagata K, Katsura K, Katayama Y, Asoh S, Ohta S. Hydrogen acts as a therapeutic antioxidant by selectively reducing cytotoxic oxygen radicals. Nat Med 2007; 13(6): 688–94.

Ohta S. Molecular hydrogen is a novel antioxidant to efficiently reduce oxidative stress with potential for the improvement of mitochondrial diseases. Biochim Biophys Acta 2012; 1820(5): 586-94.

Huang C, Kawamura T, Toyoda Y, Nakao A. Recent advances in hydrogen research as a therapeutic medical gas. Free Radic Res 2010; 44(9): 971-82.

Itoh T, Hamada N, Terazawa R, Ito M, Ohno K, Ichihara M, Nozawa Y, Ito M. Molecular hydrogen inhibits lipopolysaccharide/interferon c-induced nitric oxide production through modulation of signal transduction in macrophages. Biochem Biophys Res Commun 2011; 411(1): 143-9.

Itoh T, Fujita Y, Ito M, Masuda A, Ohno K, Ichihara M, Kojima T, Nozawa Y, Ito M. Molecular hydrogen suppresses FcepsilonRImediated signal transduction and prevents degranulation of mast cells. Biochem Biophys Res Commun 2009; 389(4): 651-6.


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