Increased THC, DHC, PA and Survival of White Shrimp (Litopenaeus vannamei) by Feeding Porphyridium cruentum Nanoparticle
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A common disease in white shrimp is vibriosis which can cause acute hepatopancreatic necrosis or Acute Hepatopancreatic Necrosis Disease (AHPND). Immunostimulants are one of the alternatives to defend against pathogen infection. Porphyridium cruentum is a natural substance that can be used as an immunostimulant. The development of nanotechnology is progressing rapidly today due to its important role in various fields. The use of P. cruentum nanoparticles enhances the absorption and efficiency of feed, making their application more effective. The purpose of this study is to determine the effect of the addition of P.cruentum nanoparticles in feed on the enhancement of the immune response of white shrimp after administration for one month of rearing. Shrimp were divided into four groups, one group was not fed with P.cruentum nanoparticles (K), three other groups were fed with P.cruentum nanoparticles 0.8; 1.0; 1.2% per kg of feed respectively. The results showed that the treatment with the addition of P.cruentum nanoparticles provided an increase in Total Hemocytes Count (THC), Differential Hemocyte Cells (DHC), and phagocytic activity (PA) after administration for one month of rearing and at the end of rearing provided a higher survival rate than the control.
Arayamethakorn, S., Uengwetwanit, T., Karoonuthaisiri, N., Methacanon, P. and Rungrassamee, W., 2023. Comparative effects of different bacterial lipopolysaccharides on modulation of immune levels to improve survival of the black tiger shrimp. Journal of Invertebrate Pathology, 197, 107872. https://doi.org/10.1016/j.jip.2022.107872
Cantelli, L., Goncalves, P., Guertler, C., Kayser, M., Pilotto, M.R., Barracco, M.A. and Perazzolo, L.M., 2019. Dietary supplementation with sulfated polysaccharides from Gracilaria birdiae promotes a delayed immunostimulation in marine shrimp challenged by the white spot syndrome virus. Aquaculture International, 27, pp.349-367. https://doi.org/10.1007/s10499-018-0328-1
Darwantin, K., Sidik, R. and Mahasri, G., 2016. Efisiensi penggunaan imunostimulan dalam pakan terhadap laju pertumbuhan, respon imun dan kelulushidupan udang vannamei (Litopenaeus vannamei). Jurnal Biosains Pascasarjana, 18(2), pp.123-139. https://doi.org/10.20473/jbp.v18i2.2016.123-139
Ermawati, R. and Ratnawati, E., 2011. Sintesis Nanopartikel Magnetit Dengan Metode Dekomposisi Termal. Jurnal Kimia Dan Kemasan, 33(1), pp.96-101. https://doi.org/10.24817/jkk.v33i1.1834
Hidayat, C., Sumiati, Wina, E. and Jayanegara, A., 2018. Pembuatan Nanopartikel Zink Menggunakan Ekstrak Tanaman untuk Imbuhan Pakan Ayam Pedaging. Watazoa, 28(3), pp.107-118. http://dx.doi.org/10.14334/wartazoa.v28i3.1833
Himzanah, S.S., Rudi, M., Prasetiyo, H. and Hartana, N.S., 2023. Perbandingan Imunostimulan yang berbeda terhadap gambaran darah udang vaname (Litopenaeus vannamei) di tambak pendampingan PT Suri Tani Pemuka. Journal of Indonesian Tropical Fisheries (JOINT-FISH): Jurnal Akuakultur, Teknologi dan Manajemen Perikanan Tangkap dan Ilmu Kelautan, 6(2), pp.110-122. https://jurnal.fpik.umi.ac.id/index.php/JOINT-FISH/article/view/200
Indraswati, V.O., Supono, S. and Saefulloh, A., 2015. Suplementasi Minyak IKan untuk Peningkatan Imunitas Non Spesifik Udang Vaname (Litopenaeus vannamei). AQUASAINS, 3(2), pp.273-278. https://jurnal.fp.unila.ac.id/index.php/JPBP/article/view/723/665
Jannah, M., Junaidi, M., Setyowati, D.N.A. and Azhar, F., 2018. Pengaruh pemberian Lactobacillus sp. dengan dosis yang berbeda terhadap sistem imun udang vaname (Litopenaeus vannamei) yang diinfeksi bakteri Vibrio parahaemolyticus. Jurnal Kelautan: Indonesian Journal of Marine Science and Technology, 11(2), pp.140-150. http://dx.doi.org/10.21107/jk.v11i2.3980
Maftuch, Prasetio, E., Sudianto, A., Rozik, M., Nurdiyani, R., Sanusi, E., Nursyam, H., Fariedah, F., Marsoedi and Murachman, 2013. Improvement of innate immune responses and defense activity in tiger shrimp (Penaeus monodon Fab.) by intramuscular administration of the outer membrane protein Vibrio alginolyticus. SpringerPlus, 2, 432. https://doi.org/10.1186/2193-1801-2-432
Manoppo, H., 2014. Respon imun nonspesifik dan pertumbuhan udang vaname (Litopenaeus vannamei) yang diberi pakan yang ditambahkan nukleotida dengan lama pemberian berbeda. e-Journal BUDIDAYA PERAIRAN, 2(1), pp.30-37. https://doi.org/10.35800/bdp.2.1.2014.3790
Mosaddegh, E. and Hassankhani, A., 2014. Preparation and characterization of nano-CaO based on eggshell waste: Novel and green catalytic approach to highly efficient synthesis of pyrano [4, 3-b] pyrans. Chinese Journal of Catalysis, 35(3), pp.351-356. https://doi.org/10.1016/S1872-2067(12)60755-4
Mursal, I.L.P., 2018. Karakterisasi Xrd dan SEM pada material nanopartikel serta peran material nanopartikel dalam drug delivery system. Pharma Xplore: Jurnal Sains dan Ilmu Farmasi, 3(2), pp.214-221. https://doi.org/10.36805/jpx.v3i2.491
Mutmainnah, N., Risjani, Y. and Hertika, A.M.S., 2018. Growth Rate and Chemical Composition of Secondary Metabolite Extracellular Polysaccharide (EPS) Microalga Porphyridium cruentum. The Journal of Experimental Life Science, 8(2), pp.97-102. https://doi.org/10.21776/ub.jels.2018.008.02.05
Ozório, R.A., Lopes, R.G., Goes, B.S., da Silva, C.P., Derner, R.B. and Fracalossi, D.M., 2015. Growth and enzymatic profile of the pacific white shrimp fed with Porphyridium cruentum extract. Boletim do Instituto de Pesca, 41(1), pp.123-131. https://institutodepesca.org/index.php/bip/article/view/41_1_123-131/41_1_123-131
Prastiti, L.A., Verdian, A.H., Oktavian, A., Fatimah, N., Fathurohman, K., Astria, Q. and Siburian, A.F., 2023. Peningkatan Respon Imun Udang Vaname (Litopenaeus vannamei) Melalui Kombinasi Vitamin D3, Mineral Ca Dan Mg Pada Pakan. Jurnal Ilmu-ilmu Perikanan dan Budidaya Perairan, 18(1), pp.14-24. https://doi.org/10.31851/jipbp.v18i1.11326
Risjani, Y., Mutmainnah, N., Manurung, P., Wulan, S.N. and Yunianta, 2021. Exopolysaccharide from Porphyridium cruentum (purpureum) is not toxic and stimulates immune response against vibriosis: the assessment using zebrafish and white shrimp Litopenaeus vannamei. Marine Drugs, 19(3), 133. https://doi.org/10.3390/md19030133
Rosyida, A., Setyowati, D.N.A. and Azhar, F., 2022. The effect of adding white tumeric (Curcuma zedoaria) extract on the immune system of vaname shrimp (Litopenaeus vannamei) tested against Vibrio harveyi bacteria. Jurnal Perikanan dan Kelautan, 27(2), pp.136-144. https://doi.org/10.31258/jpk.27.2.136-144
Rustam, Nursyahran and Hartinah, 2024. The Cellular Response of the Juvenile White Shrimp (Lithopenaus vannamei) to Rearing with Different Levels of Cultivation Technology. Egyptian Journal of Aquatic Biology & Fisheries, 28(3), pp.1401-1409. https://ejabf.journals.ekb.eg/article_362762_8d341701562b72feab880571cd2fa95b.pdf
Satyantini, W.H., Kurniawan, A. and Kusdarwati, R., 2016. Penambahan Ekstrak Gracilaria verrucosa Terhadap Peningkatan Total Hemosit, Kelangsungan Hidup dan Respon Fisiologi Udang Galah (Macrobrachium rosenbergii). Jurnal Akuatika Indonesia, 1(2), pp.120-129. https://jurnal.unpad.ac.id/akuatika-indonesia/article/view/29158
Setyaningsih, I., Salamah, E. and Rahman, D.A., 2013. Chemical composition and Antihiperglychemic Activity of Biomass and Extracelluler Polysaccharide of Microalgae Porphyridium cruentum. Jurnal Pengolahan Hasil Perikanan Indonesia, 16(1), pp.79-85. https://doi.org/10.17844/jphpi.v16i1.7777
Syaichudin, M., Akmal, Hasbullah, D., Sirajuddin and Hartanto, N., 2019. Prospek Pengembangan Induk Udang Vaname Nusantara (Litopenaeus vannamei) Secara Berkelanjutan di BPBAP Takalar. Jurnal Perekayasaan Akuakultur Indonesia, 1(3), pp.21-37. https://www.researchgate.net/publication/358586379_PROSPEK_PENGEMBANGAN_INDUK_UDANG_VANAME_NUSANTARA_Litopenaeus_vannamei_SECARA_BERKELANJUTAN_DI_BPBAP_TAKALAR?_sg=-JKp6Xp2rrEFJVw72IOYp8m8aI3p2bjDraNvBbBHm2MSFjf3ygWJd9dYo8UkYGH20s-jvvu_jy3CDVY&_tp=eyJjb250ZXh0Ijp7ImZpcnN0UGFnZSI6InB1YmxpY2F0aW9uIiwicGFnZSI6Il9kaXJlY3QifX0
Torky, A., Saad, S. and Eltanahy, E., 2023. Microalgae as dietary supplements in tablets, capsules, and powder. In Handbook of food and feed from microalgae (pp.357-369). Academic Press. https://doi.org/10.1016/B978-0-323-99196-4.00002-4
Tseng, C.C., Chu, T.W., Danata, R.H., Risjani, Y., Shih, H.T. and Hu, S.Y., 2020. Hepcidin-expressing fish eggs as a novel food supplement to modulate immunity against pathogenic infection in Zebrafish (Danio rerio). Sustainability, 12(10), 4057. https://www.mdpi.com/2071-1050/12/10/4057
Zhang, Z., Shi, X., Wu, Z., Wu, W., Zhao, Q. and Li, E., 2023. Macroalgae improve the growth and physiological health of white shrimp (Litopenaeus vannamei). Aquaculture Nutrition, 2023(1), 8829291. https://doi.org/10.1155/2023/8829291
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