EKSPLORASI GEN ENZIM LIPASE PADA TANAH PENGOLAHAN LIMBAH KELAPA SAWIT DENGAN PENDEKATAN METAGENOMIK
Downloads
Abstrak
Enzim lipase merupakan bagian dari enzim hidrolase yang bekerja pada ikatan ester. Enzim ini juga mengkatalisis beberapa jenis reaksi sehingga merupakan enzim yang potensial diaplikasikan ke berbagai bidang seperti industri tekstil, kulit, deterjen, makanan, dan lain-lain. Salah satu cara mendapatkan enzim novel ialah dengan pendekatan secara metagenomik dari sampel lingkungan tanpa melalui kultur di dalam laboratorium. Sampel lingkungan yang biasa diteliti ialah memiliki keadaan ekstrem atau memiliki sumber substrat enzim yang ingin dieksplorasi. Pada penelitian ini dilakukan eksplorasi gen enzim lipase pada tanah hasil pengolahan limbah kelapa sawit (POME) melalui pendekatan metagenomik menggunakan desain primer degenerate untuk gen enzim lipase untuk golongan HSL. Didapatkan fragmen gen lipase yang memiliki ukuran 250-300 pasang basa dan dilakukan kloning ke dalam plasmid pET-30a(+) dengan sel inang E. coli TOP10 menghasilkan pustaka fragmen lipase limbah sawit (PFL2S) sebanyak 26 klon.
Kata kunci: Lipase, metagenomik, primer degenerate
Glogauer, A., Martini, V.P., Faoro, H., Couto, G.H., Müller-santos, M., Monteiro, R.A., Mitchell, D.A., Souza, E.M. De, Pedrosa, F.O., Krieger, N., 2011. Identification and characterization of a new true lipase isolated through metagenomic approach. Microb. Cell Fact. 10, 54.
Handelsman, J., Rondon, M.R., Brady, S.F., Clardy, J., Goodman, R.M., 1998. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem. Biol. 5, R245–R249.
Hurt, R.A., Qiu, X., Wu, L., Roh, Y., Tiedje, J.M., Zhou, J., Hurt, R.A., Qiu, X., Wu, L., Roh, Y.U.L., Palumbo, A. V, 2001. Simultaneous Recovery of RNA and DNA from Soils and Sediments. Appl. Environ. Microbiol. 67.
JunGang, L., KeGui, Z., WenJun, H., 2010. Cloning and biochemical characterization of a novel lipolytic gene from activated sludge metagenome, and its gene product. Microb. Cell Fact. 9, 83.
Kakirde, K.S., Parsley, L.C., Liles, M.R., 2010. Size does matter: Application-driven approaches for soil metagenomics. Soil Biol. Biochem. 42, 1911–1923.
Lämmle, K., Zipper, H., Breuer, M., Hauer, B., Buta, C., Brunner, H., Rupp, S., 2007. Identification of novel enzymes with different hydrolytic activities by metagenome expression cloning. J. Biotechnol. 127, 575–592.
Liaw, R.-B., Cheng, M.-P., Wu, M.-C., Lee, C.-Y., 2010. Use of metagenomic approaches to isolate lipolytic genes from activated sludge. Bioresour. Technol. 101, 8323–9.
Lorenz, P., Eck, J., 2005. Metagenomics and industrial applications. Nature 3, 510–516.
Momsia, T., Momsia, P., 2013. A REVIEW ON " MICROBIAL LIPASE-VERSATILE TOOL FOR INDUSTRIAL APPLICATIONS .” Int. J. life Sci. Biotechnol. pharma Res. 2.
Otten, L.G., Quax, W.J., 2005. Directed evolution: selecting today's biocatalysts. Biomol. Eng. 22, 1–9.
Schoemaker, H.E., 2003. Dispelling the Myths--Biocatalysis in Industrial Synthesis. Science (80-. ). 299, 1694–1697.
Shi, P., Liu, W., Meng, K., Bai, Y., Wang, G., Zhan, Z., Yao, B., 2009. Lipase Diversity in Glacier Soil Based on Analysis of Metagenomic DNA Fragments and Cell Culture 19, 888–897.
Simon, C., Daniel, R., 2009. Achievements and new knowledge unraveled by metagenomic approaches. Appl. Microbiol. Biotechnol. 85, 265–276.
Steele, H.L., Jaeger, K.-E., Daniel, R., Streit, W.R., 2009. Advances in Recovery of Novel Biocatalysts from Metagenomes. J. Mol. Microbiol. Biotechnol. 16, 25–37.
Uchiyama, T., Miyazaki, K., 2009. Functional metagenomics for enzyme discovery: challenges to efficient screening. Curr. Opin. Biotechnol. 20, 616–622.
Yan, Q., Duan, X., Liu, Y., Jiang, Z., Yang, S., 2016. Biotechnology for Biofuels Expression and characterization of a novel 1 , 3 "‘ regioselective cold "‘ adapted lipase from Rhizomucor endophyticus suitable for biodiesel synthesis. Biotechnol. Biofuels 1–13.
Copyright (c) 2017 Jurnal Kimia Riset
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
COPYRIGHT NOTICE
1. By submitting the article to Jurnal Kimia Riset (JKR), the author has agreed to transfer some of the copyrights to the publisher of the research chemistry journal, Universitas Airlangga, indicated in the Copyright Transfer Agreement.
2. Authors still retain significant rights to use and share their own published articles for non-commercial purposes subject to Creative Commons Attribution-NonComercial 4.0 International License
3. All publications (printed/electronic) are open access for educational purposes, research, library, and other non-commercial purposes. Besides the purposes mentioned above, the editorial board is not responsible for copyright violations.
