Pengaruh Kombinasi Metformin dan Ekstrak Air Meniran (Phyllanthus niruri Linn.) terhadap Perbaikan Status Obesitas Tikus Sprague dawley Jantan
Downloads
Latar Belakang: Perubahan gaya hidup dan pola makan menyebabkan tingginya angka kejadian obesitas di seluruh dunia yaitu 13% padat tahun 2016. Perbaikan status obesitas dapat dilakukan melalui jalur farmakologik ataupun non farmakologik. Metformin dan meniran (Phyllanthus niruri Linn.) mempunyai fungsi untuk menurunkan penyerapan asupan makanan.
Tujuan: menganalisis pengaruh kombinasi metformin dan ekstrak air meniran (Phyllanthus niruri Linn.) terhadap perbaikan status obesitas tikus Sprague Dawley jantan.
Metode: multigroup time series design. Dua puluh empat ekor tikus dibuat obesitas dengan cara diberi pakan tambahan lemak sapi cair dan fruktosa terhadap pakan dasar AIN93G selama 5 minggu. Obesitas ditentukan dengan indeks Lee > 300. Sampel dikelompokkan menjadi 4 kelompok, kelompok 1 diberi metformin 45 mg/kg BB/hari, kelompok 2 diberi ekstrak air meniran 400 mg/kg BB/hari, kelompok 3 diberi kombinasi keduanya, dan kelompok 4 kontrol.
Hasil: Selama penelitian ada perbedaan berat pakan dan asupan kalori pada setiap kelompok perlakuan. Setelah 28 hari perlakuan ada perbedaan kenaikan berat badan dan perubahan indeks Lee antara ketiga kelompok perlakuan (Ï < 0,05), walaupun indeks Lee > 300.
Kesimpulan: pemberian kombinasi ekstrak air meniran dan metformin dapat mengendalikan kenaikan berat badan dan menurunkan indeks Lee.Astrup, A. The satiating power of protein ” a key to obesity prevention ? 1 , 2. 1–2 (2005).
A, A. A. S. M. S. M. K. Prevention and treatment of obesity -- an over view. TT -. Pakistan J. Nutr. 3, 310–317 (2004).
WHO. Obesity and overweight. (2018).
Kemenkes Republik Indonesia. Potret sehat indonesia dari riskesdas 2018. (2018).
Kementrian Kesehatan Republik Indonesia. Hasil Utama Riskesdas 2018. (Kementrian Kesehatan RI, 2018).
WHO. Guideline : Daily iron and folic acid supplementation in pregnant women. WHO (2012).
Adisapoetra, I. Strategi peningkatan aktivitas fisik. in (Widyakarya Nasional Pangan dan Gizi IX, 2008).
Cheng, D. Prevalence, predisposition and prevention of type II diabetes. Nutr. Metab. (Lond). 2, 1–12 (2005).
Beatriz, L., Rojas, A. & Gomes, M. B. Metformin : an old but still the best treatment for type 2 diabetes. Diabetol. Metab. Syndr. 5, 1 (2013).
Azcona-sanjulián, M. C., Lambán, A. C. & Ruiz, B. L. Six-Month Therapy with Metformin in Association with Nutritional and Life Style Changes Obesity in Children and Adolescents with Obesity. Int. J. Pediatr. Res. 1, 1–6 (2015).
LS, H. Metformin: a review of its pharmacological properties and therapeutic use. Diabete Metab. 5, 233–45 (1979).
Mellinger, C. G. et al. Chemical and biological properties of an arabinogalactan from Phyllanthus niruri. J. Nat. Prod. 68, 1479–1483 (2005).
Okoli, C., Ezike, A. & Akah, P. Evaluation of antidiabetic potentials of Phyllanthus niruri in alloxan diabetic rats Evaluation of antiplasmodial natural products View project Natural Products View project. African J. Biotechnol. 9, 248–259 (2010).
S. Thiangthuma, B. Dejaeghera, M. Goodarzia, C. Tistaerta, A.Y. Gordienc, N. Nguyen Hoaid, M. Chau Vand, J. Quetin-Leclercqc, L. Suntornsukb, Y. V. H. Potentially antioxidant compounds indicated from Mallotus and Phyllanthus species fingerprints. J. Chromatogr. 114–121 (2012).
Adeneye, A. A., Amole, O. O. & Adeneye, A. K. Hypoglycemic and hypocholesterolemic activities of the aqueous leaf and seed extract of Phyllanthus amarus in mice. Fitoterapia 77, 511–514 (2006).
Shetti, A. A., Sanakal, R. D. & Kaliwal, B. B. Antidiabetic effect of ethanolic leaf extract of Phyllanthus amarus in alloxan induced diabetic mice. 2, 11–15 (2012).
Asare, G. A. et al. Acute toxicity studies of aqueous leaf extract of Phyllanthus niruri. Interdiscip. Toxicol. 4, 206–210 (2011).
Oyewo, Bukoye, E., Akanji, Adewumi, M. & Adekunle, A. S. Immunomodulation Capabilities of Aqueous Leaf Extract of Phyllanthus amarus in male Wistar Rats. Rep. Opin. 4, 22–37 (2012).
Reeves, P. G. Committee Report AIN-93 Purified Diets for Laboratory Rodents : Final Report of the American Institute of Nutrition Ad Hoc Writing Committee on the Reformulation of the AIN-76A Rodent Diet. 1939–1951 (1993).
Rogers, P. & Webb, G. P. Estimation of body fat in normal and obese mice. Br. J. Nutr. 43, 83 (1980).
Giribabu, N. et al. Aqueous Extract of Phyllanthus niruri Leaves Displays In Vitro Antioxidant Activity and Prevents the Elevation of Oxidative Stress in the Kidney of Streptozotocin-Induced Diabetic Male Rats. 2014, (2014).
S., C., H, E., J, B. & B, S. Obesity: Handbook of General Practice. (Oxford University Press, 2002).
Ogunbode, A. ., Ogunbode, O. & Fatiregun, A. Health risk obesity. 7, 7–10 (2009).
Malin, S. K. & Kashyap, S. R. Effects of metformin on weight loss : potential mechanisms. 323–329 (2014). doi:10.1097/MED.0000000000000095
Seifarth, C., Schehler, B., Schneider, H. J., Clin, E. & Diabetes, E. E ff ectiveness of Metformin on Weight Loss in Non-Diabetic Individuals with Obesity. 27–31 (2012).
Tiwari, A. K. & Rao, J. M. Diabetes mellitus and multiple therapeutic approaches of phytochemicals : Present status and future prospects. (2002).
Khanna, A. K., Rizvi, F. & Chander, R. Lipid lowering activity of Phyllanthus niruri in hyperlipemic rats. J. Ethnopharmacol. 82, 19–22 (2002).
Karuna, R., Reddy, S. S., Baskar, R. & Saralakumari, D. Antioxidant potential of aqueous extract of Phyllanthus amarus in rats. Indian J. Pharmacol. 41, 64–7 (2009).
AMERTA NUTR by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under "the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.
