The Effect of Trehalose Sugar on Insulin Resistance in Old Rats by Assessing HOMA-IR (Homeostasis Model Assessment-Insulin Resistance)
Downloads
Background: Insulin resistance is a condition in which insulin cannot take up glucose, increasing blood glucose. Elderly people are more exposed to insulin resistance, requiring dietary interventions that extend longevity. Trehalose, a naturally occurring sugar, showed potentially reduce insulin resistance which can be measured using the HOMA-IR index.
Objectives: This study aimed to assess HOMA-IR levels as a parameter of insulin resistance in old rats after giving trehalose sugar.
Methods: Experimental research with 28 male Wistar rats (Rattus novergicus) was separated into 4 groups, the control group of young rats (Group A), the control group of old rats (Group B), a group of old rats that were given 2% Trehalose solution (Group C), and a group of old rats that given 2% sucrose solution (Group D) that observed for 8 weeks.
Results: The results showed differences in HOMA-IR levels (p<0.001) between old and young subjects. The intervention in Group C was optimal in reducing levels of HOMA-IR (p<0.001) by 18.2% compared with the old control, while Group D increased levels of HOMA-IR by 14.3% (p<0.001) compared with the old control. The age of the subjects with HOMA-IR level is positively correlated (p<0.001; r=0.721) and the weight of subjects with the HOMA-IR level is also positively correlated (p<0.001; r=0.698), indicating that the older and the greater weight of subject resulting in the bigger of HOMA-IR value.
Conclusion: Trehalose is effective in reducing HOMA-IR levels as a parameter of insulin resistance in old rats.
Barzilai, N. & Ferrucci, L. Insulin resistance and aging: a cause or a protective response? Journals Gerontol. Ser. A Biomed. Sci. Med. Sci. 67, 1329–1331 (2012).
Subramaniam, I. & Gold, J. Diabetes Mellitus in Erderly. J. Indian Acad Geri 2, 77–81 (2005).
Fink, R. I., Kolterman, O. G., Griffin, J. & Olefsky, J. M. Mechanisms of insulin resistance in aging. J. Clin. Invest. 71, 1523–1535 (1983).
Widiastuti, N. H. Faktor Risiko yang Mempengaruhi Terjadinya Resistensi Insulin pada Wanita Lansia 58-65 Tahun di Kounitas Lansia Restu RSUD Dr Soetomo Surabaya. (Universitas Airlangga, 2019).
Ryan, A. S. Insulin Resistance with Aging. Sport. Med. 30, 327–346 (2000).
DeBosch, B. J. et al. Trehalose inhibits solute carrier 2A (SLC2A) proteins to induce autophagy and prevent hepatic steatosis. Sci Signal 146, 1–28 (2017).
Arai, C. et al. Trehalose prevents adipocyte hypertrophy and mitigates insulin resistance in mice with established obesity. J. Nutr. Sci. Vitaminol. (Tokyo). 59, 393–401 (2013).
Yoshizane, C. et al. Daily consumption of one teaspoon of trehalose can help maintain glucose homeostasis: A double-blind, randomized controlled trial conducted in healthy volunteers. Nutr. J. 19, 1–9 (2020).
Sengupta, P. The laboratory rat: Relating its age with human's. Int. J. Prev. Med. 4, 624–630 (2013).
Federer, W. . Experimental Design, Theory and Application. Oxford and IBH Publ. Co (Oxford and IBH Publ. Co, 1967).
Yu, W. et al. Trehalose Protects against Insulin Resistance-Induced Tissue Injury and Excessive Autophagy in Skeletal Muscles and Kidney. Curr. Pharm. Des. 25, 2077–2085 (2019).
Antunes, L. C., Elkfury, J. L., Jornada, M. N., Foletto, K. C. & Bertoluci, M. C. Validation of HOMA-IR in a model of insulin-resistance induced by a high-fat diet in Wistar rats. Arch. Endocrinol. Metab. 60, 138–142 (2016).
Pagliassotti, M. J. et al. Trehalose supplementation reduces hepatic endoplasmic reticulum stress and inflammatory signaling in old mice. J. Nutr. Biochem. 45, 15–23 (2017).
Hermanto, T., Sony, W. & DPP, B. Korelasi antara HOMA-IR Ibu Diabetes Mellitus Gestasional Trimester Tiga dengan Luaran Maternal dan Neonatal. Maj. Obstet. Ginekol. 20, 122–126 (2012).
Sah, S. P., Singh, B., Choudhary, S. & Kumar, A. Animal models of insulin resistance: A review. Pharmacol. Reports 68, 1165–1177 (2016).
Jura, M. & Kozak, L. P. Obesity and related consequences to aging. Age (Omaha). 38, (2016).
Berry, A. et al. Trehalose administration in C57BL/6N old mice affects healthspan improving motor learning and brain anti-oxidant defenses in a sex-dependent fashion: a pilot study. Exp. Gerontol. 129, 110755 (2020).
O'Neill, M. K., Piligian, B. F., Olson, C. D., Woodruff, P. J. & Swarts, B. M. Tailoring Trehalose for Biomedical and Biotechnological Applications. Pure Appl Chem 89, 1223–1249 (2017).
Sheludiakova, A., Rooney, K. & Boakes, R. A. Metabolic and behavioural effects of sucrose and fructose/glucose drinks in the rat. Eur. J. Nutr. 51, 445–454 (2012).
Copyright (c) 2022 Amerta Nutrition
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
AMERTA NUTR by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under "the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.
