Hypolipidemic Effects of Modified Edamame Tempeh Flour on Lipid Profile Levels in Dyslipidemia Rats
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Background: Dyslipidemia is a lipid metabolism disorder that causes an increase or decrease in plasma lipid concentrations. Comprehensive management is an attempt to prevent and reduce dyslipidemia, one of which is nutritional therapy. Edamame contains nutrients such as protein, fat, fiber, and isoflavones that have the potential to improve lipid profiles. Edamame tempeh production is one way to improve product quality that is improving nutritional quality, digestibility, and bioavailability. Edamame tempeh modification is conducted by adding yeast Saccharomyces cerevisiae. This yeast plays a role in increasing the isoflavone levels and the product containing β-glucan.
Objectives: This research aims to analyze the effects of edamame tempeh flour (ET) and modified edamame tempeh flour (MET) on lipid profile levels in dyslipidemic rats.
Methods: This research was a true experimental with a randomized controlled group pretest-posttest design. Thirty-six Sprague Dawley male white rats that met the inclusion criteria were randomized and divided into six treatment groups consisting of negative control, positive control, the dose of ET flour was 2.7 g/200gBW and 5.4 g/200gBW, the dose of MET flour was 2.7 g/200gBW and 5.4 g/200gBW. The intervention was carried out once per day in the morning for 28 days. The parameters observed included total cholesterol, LDL-C, and HDL-C levels measured by the CHOP-PAP method and triglyceride levels using the GPO-PAP method with a spectrophotometer measuring instrument. Examination of lipid profile levels was carried out three times. All statistical tests used a 95% significance level.
Results: There was a significant difference in the decrease in total cholesterol, LDL-C, and triglyceride levels as well as an increase in HDL-C levels compared to before the intervention (p<0.05).
Conclusions: The administration of ET flour and MET flour could significantly reduce total cholesterol, LDL-C, triglyceride, and increased HDL-C, but the administration of MET flour, especially at a dose of 5.4 g/200gBW, showed a more effective improvement in lipid profile and approached the positive control group compared to ET flour.
Moor, V. J. A., Amougou, S. N., Ombotto, S., Ntone, F., Wouamba, D. E. & Nonga, B. N. Dyslipidemia in Patients with a Cardiovascular Risk and Disease at the University Teaching Hospital of Yaoundé, Cameroon. Int. J. Vasc. Med. 2017, 1–5 (2017).
Ayoade, O. G., Umoh, I. & Amadi, C. Dyslipidemia and Associated Risk Factors among Nigerians with Hypertension. Dubai Med. J. 3, 155–161 (2020).
Laila, U. & Hermawan, R. Kejadian Hiperkolesterolemia pada Pelaut PT. Samudera Indonesia. Bid. Ilmu Kesehat. 5, 270–276 (2015).
Fitriyana, N. I. Potency of functional food from edamame as Hipocholesterolemic Food. J. Rekapangan 11, 10–19 (2017).
Gulati, S., Misra, A. & Pandey, R. M. Effects of 3 g of soluble fiber from oats on lipid levels of Asian Indians - a randomized controlled, parallel arm study. Lipids Health Dis. 16, 1–8 (2017).
Rhee, E., Kim, H. C., Kim, J. H., Lee., E. Y., Kim, B. J., Kim, E, M., Song, Y. J., Lim, J. H., Kim, H. J., Choi, S., Moon, M. K., Na, J. O., Park, K., Oh, M, S., Han., S, Y., Noh, J., Yi, K, H., Lee, S., Hong, S. & Jeong, I. 2018 Guidelines for the management of dyslipidemia Cardiovascular disease in Koreans. Korean J. Intern. Med. 34, 723–771 (2019).
Priastiti, D. A. & Puruhita, N. Perbedaan Kadar Kolesterol LDL Penderita Dislipidemia pada Pemberian Tempe Kedelai Hitam dan Tempe Kedelai Kuning. J. Nutr. Coll. 2, 262–276 (2013).
Ahnan-Winarno, A. D., Cordeiro, L., Winarno, F. G., Gibbons, J. & Xiao, H. Tempeh: A semicentennial review on its health benefits, fermentation, safety, processing, sustainability, and affordability. Compr. Rev. Food Sci. Food Saf. 20, 1717–1767 (2021).
Fitranti, D. Y. & Marthandaru, D. Pengaruh susu kedelai dan jahe terhadap kadar kolesterol total pada wanita hiperkolesterolemia. J. Gizi Indones. (The Indones. J. Nutr. 4, 89–95 (2016).
Kurniawan, N. D., Setiani, B. E. & Dwiloka, B. Kadar Lemak, Kadar Air, Kadar Protein, dan Antioksidan Tempe Edamame (Glycine max (L) Merrill) Dengan Jenis Pengemas Yang Berbeda. J. Teknol. Pangan 3, 355–358 (2019).
Maruthi, J. & Paramesh, R. Effect of integrated nutrient management on seed quality of vegetable soybean [Glycine max (L.) merrill] cv. Karune. Legum. Res. 39, 578–583 (2016).
Xu, Y., Cartier, A., Kibet, D., Jordan, K., Hakala, I., Davis, S., Sismour, E., Kering, M. & Rutto, L. Physical and nutritional properties of edamame seeds as influenced by stage of development. J. Food Meas. Charact. 10, 193–200 (2016).
Zeipiņa, S., Alsiņa, I. & Lepse, L. Insight in edamame yield and quality parameters: A review. in Research for Rural Development vol. 2 40–44 (2017).
Multari, S., Stewart, D. & Russell, W. R. Potential of Fava Bean as Future Protein Supply to Partially Replace Meat Intake in the Human Diet. Compr. Rev. Food Sci. Food Saf. 14, 511–522 (2015).
Radiati, A. & Sumarto. Analisis Sifat Fisik , Sifat Organoleptik, dan Kandungan Gizi pada Produk Tempe dari Kacang Non-Kedelai. J. Apl. Teknol. Pangan 5, 16–22 (2016).
Ali, N. M., Yeap, S. K., Yusof, H. M., Beh, B. K., Ho, W. Y., Koh, S. P., Abdullah, M. P., Alitheen, N. B. & Long, K. Comparison of free amino acids, antioxidants, soluble phenolic acids, cytotoxicity and immunomodulation of fermented mung bean and soybean. J Sci Food Agric 96, 1648–1658 (2015).
Astawan, M., Mardhiyyah, Y. S. & Wijaya, C. H. Potential of Bioactive Components in Tempe for the Treatment of Obesity. J. Gizi dan Pangan 13, 79–86 (2018).
Kustyawati, M. E., Nawansih, O. & Nurdjanah, S. Profile of aroma compounds and acceptability of modified tempeh. Int. Food Res. J. 24, 734–740 (2017).
Santos, V. A. Q. Nascimento, C. G., Schmidt, C. A. P., Mantovani, D., Dekker, R. F. H. & Cunha, M. A. da. Solid-state fermentation of soybean okara: Isoflavones biotransformation, antioxidant activity and enhancement of nutritional quality. Lwt 92, 509–515 (2018).
Pengkumsri, N., Suvamaruthi, B. S., Sirilun, S., Peerajan, S., Kesika, P., Chaiyasut, K. & Chaiyasut, C. Extraction of β-glucan from Saccharomyces cerevisiae: Comparison of different extraction methods and in Vivo assessment of immunomodulatory effect in mice. Food Sci. Technol. 37, 124–130 (2017).
Henrion, M., Francey, C., Lê, K. A. & Lamothe, L. Cereal B-glucans: The impact of processing and how it affects physiological responses. Nutrients 11, 1–14 (2019).
Rizal, S., Kustyawati, M. E., Murhadi & Hasanudin, U. The Growth of Yeast and Fungi, the Formation of β-Glucan, and the Antibacterial Activities during Soybean Fermentation in Producing Tempeh. Int. J. Food Sci. 1–8 (2021) doi:10.1155/2021/6676042.
Nurkistin, D., Tamtomo, D. G. & Wiboworini, B. International Nursing and Health Sciences Symposium. in Proximate and isoflavones content in edamame tempeh flour and modified edamame tempeh flour 14–21 (2021).
WHO. General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine World Health Organization. (2000).
Salsabila, D. M., Maryusman, T. & Fatmawati, I. Pengaruh Sinbitok Kefir Tepung Pisang Batu (Musa balbisiana) terhadap Kadar Glukosa Darah Tikus Sindrom Metabolik. J. Bioteknol. Biosains Indones. 7, 18–27 (2020).
Parameshwar, P., Naik, G. N., Kumar, N. S. & Vidyasagar, M. Pharmacokinetic Profile of Wheat Bran on Hyperlipidemic Wistar Strain Rats. Int J Biopharm. 3, 78–81 (2012).
Jafar, H., Kenta, Y. S. & Nurhaeni. Uji efek anti hiperkolesterolemia ekstrak etanol daun boroco merah terhadap tikus putih jantan hiperkolesterolemia. Farmakol. J. Farm. XVI, 26–34 (2019).
Nugraheni, K. & Bintari, S. H. Aktivitas antidislipidemia tepung tempe dan susu kedelai pada profil lipid tikus diabetes yang diinduksi streptozotocin. J. Gizi dan Diet. Indones. 4, 147–153 (2016).
Nishimura, M., Ohkawara, T., Sato, Y., Satoh, H., Takahashi, Y., Hajika, M. & Nishihira, J. Improvement of Triglyceride Levels through the Intake of Enriched-β-Conglycinin Soybean (Nanahomare) Revealed in a Randomized, Double-Blind, Placebo-Controlled Study. Nutrients 8, 1–14 (2016).
Lammi, C., Zanoni, C. & Arnoldi, A. IAVPGEVA, IAVPTGVA, and LPYP, three peptides from soy glycinin, modulate cholesterol metabolism in HepG2 cells through the activation of the LDLR-SREBP2 pathway. J. Funct. Foods 14, 469–478 (2015).
Caponio, G. R., Wang, D. Q. H., Di Ciaula, A., De Angelis, M. & Portincasa, P. Regulation of cholesterol metabolism by bioactive components of soy proteins: Novel translational evidence. Int. J. Mol. Sci. 22, 1–18 (2021).
Khoury, D. El & Anderson, G. H. Recent advances in dietary proteins and lipid metabolism. Curr Opin Lipidol 243, 207–213 (2013).
Food and Drug Administration (FDA). Food labeling health claims; soy protein and coronary heart disease. Fed Regist vol. 64 (2017).
Fairudz, A. & Nisa, K. Pengaruh Serat Pangan Terhadap Kadar Kolesterol Penderita Overweight. J. Major. 4, 121–126 (2015).
Sima, P., Vannucci, L. & Vetvicka, V. β-glucans and cholesterol (Review). Int. J. Mol. Med. 41, 1799–1808 (2018).
Huang, Y. C., Wu, B. H., Chu, Y. L., Chang, W. C. & Wu, M. C. Effects of tempeh fermentation with lactobacillus plantarum and rhizopus oligosporus on streptozotocin-induced type II diabetes mellitus in rats. Nutrients 10, 1–15 (2018).
Flórez-Méndez, J. & González, L. Role of the consumption of fucoidans and beta-glucans on human health: An update of the literature. Rev. Chil. Nutr. 46, 768–775 (2019).
Guilarducci, J. D. S., Marcelino, B. A. R., Konig, I. F. M., Orlando, T. M., Varaschin, M. S. & Pereira, L. J. Therapeutic effects of different doses of prebiotic (isolated from Saccharomyces cerevisiae) in comparison to n-3 supplement on glycemic control, lipid profiles and immunological response in diabetic rats. Diabetol. Metab. Syndr. 12, 1–12 (2020).
Ramdath, D. D., Padhi, E. M. T., Sarfaraz, S., Renwick, S. & Duncan, A. M. Beyond the cholesterol-lowering effect of soy protein: A review of the effects of dietary soy and its constituents on risk factors for cardiovascular disease. Nutrients 9, (2017).
Sagith, D. V., Ilmiawati, C. & Katar, Y. Pengaruh Pemberian Ekstrak Biji Melinjo (Gnetum gnemon) Terhadap Kadar Kolesterol LDL Pada Tikus Galur Wistar (Rattus norvegicus) Model Hiperkolesterolemia. J. Kesehat. Andalas 7, 486 (2018).
Uchendu, I. K., Onwukwe, O. S., Agu, C. E., Orji, O. C., Chekwube, B. E. & Nwosu, T. F. Hypolipidaemic and renoprotective effects of Glycine max (Soy bean) against lipid profile and renal biochemical alterations in hypercholesterolemic rat. Int. J. Biomed. Res. 7, 822–828 (2016).
Widiyawati, A. & Susindra, Y. Utilization of edamame soybean (Glycine max (L) Merril) as modified of enteral formula high calories. in IOP Conference Series: Earth and Environmental Science vol. 207 1–7 (2018).
Handayani, W. & Rudijanto, A. Mechanism of Pufas (Polyunsaturated Fatty Acids) in Soy Milk Against Insulin Resistance Improvement. J. Appl. Environ. Biol. Sci. 7, 185–190 (2017).
Kusmiati & Dhewantara, F. X. R. Cholesterol-lowering effect of beta glucan extracted from saccharomyces cerevisiae in rats. in Scientia Pharmaceutica vol. 84 153–165 (2016).
Grundy, M. M. L., Fardet, A., Tosh, S. M., Rich, G. T. & Wilde, P. J. Processing of oat: The impact on oat’s cholesterol lowering effect. Food Funct. 9, 1328–1343 (2018).
Sokoła-Wysoczańska, E., Wysoczański, T., Wagner, J., Czyz, K., Bodkowski, R., Loncyński, S. & Patkowska-Sokola, B. Polyunsaturated fatty acids and their potential therapeutic role in cardiovascular system disorders—a review. Nutrients 10, 1–21 (2018).
Astawan, M., Wresdiyati, T. & Saragih, A. M. Evaluasi Mutu Protein Tepung Tempe dan Tepung Kedelai Rebus Pada Tikus Percobaan Evaluation of Protein Nutritional Quality of Tempe and Boiled Soybean Flours by Rats. J. Mutu Pangan Indones. J. Food Qual. 2, 11–17 (2015).
Astawan, M., Wresdiyati, T. & Sirait, J. Pengaruh Konsumsi Tempe Kedelai Grobongan terhadap profil Serum, Hematologi, dan Antioksidan Tikus. J. Teknol. dan Ind. Pangan 26, 155–162 (2015).
Nachvak, S. M., Moradi, S., Anjom-Shoae, J., Rahmani, J., Nisiri, M., Maleki, V. & Sadeghi, O. Soy, Soy Isoflavones, and Protein Intake in Relation to Mortality from All Causes, Cancers, and Cardiovascular Diseases: A Systematic Review and Dose–Response Meta-Analysis of Prospective Cohort Studies. J. Acad. Nutr. Diet. 119, 1–35 (2019).
Herwana, E. & Graciela, A. High dietary daidzein intake lowers cholesterol levels among post-menopausal women. Universa Medica 39, 47–54 (2020).
Froyen, E. & Burns-Whitmore, B. nutrients The Effects of Linoleic Acid Consumption on Lipid Risk Markers for Cardiovascular Disease in Healthy Individuals : A Review of Human Intervention Trials. Nutrients 12, 1–19 (2020).
Inoue, N., Fujiwara, Y., Funayama, A., Ogawa, N., Tachibana, N., Kohno, M. & Ikeda, I. Soybean β-conglycinin improves carbohydrate and lipid metabolism in Wistar Rats. Biosci. Biotechnol. Biochem. 79, 1528–1534 (2015).
Afifah, D. N., Nabilah, N., Supradam G. T., Pratiwi, S. N., Nuryanto. & Sulchan, M. The Effects of Tempeh Gembus, an Indonesian Fermented Food, on Lipid Profiles in Women with Hyperlipidemia. Curr. Nutr. Food Sci. 16, 56–64 (2020).
Barańska, A., Blaszczuk, A., Polz-Dacewicz, M., Kanadys, W., Malm, M., Janiszewska, M. & Jedrych, M. Effects of Soy Isoflavones on Glycemic Control and Lipid Profile in Patients with Type 2 Diabetes : A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Nutrients 13, 2–19 (2021).
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