Effect of Combination of Averrhoa Bilimbi Extract and Curcuma Longa Extract on Low Density Lipoprotein Levels in Rats Fed a High Fat Diet
Pengaruh Kombinasi Ekstrak Belimbing Wuluh (Averrhoa Bilimbi L.) dan Ekstrak Kunyit (Curcuma Longa L.) terhadap Kadar Low Density Lipoprotein
Downloads
Background: Hypercholesterolemia is a state of total blood cholesterol levels transported by LDL exceeding normal limits. As a result, it can clog blood vessels, and this condition is a risk factor for CHD. Alternative medicine includes flavonoids in Averrhoa bilimbi (belimbing wuluh) known to reduce cholesterol and LDL levels, and curcumin in turmeric known to reduce fat absorption and increase fat excretion.
Objectives: The aim of this study was to analyze the combined effect of bilimbi fruit and turmeric extracts on LDL levels.
Methods: This experimental research used post-test only with a control group design involving male Wistar rats divided into 6 (six) random groups. Group KN (standard feed, aquadest for 28 days), K- (high-fat feed for 14 days, followed by standard feed and aquadest), K+ (high-fat feed for 14 days, followed by standard feed, aquadest, and simvastatin 0.18mg/200 g BW/day), P1 (high-fat feed for 14 days, followed by standard feed, aquadest and extracts of bilimbi fruit and turmeric extract at a dose of 240: 270 mg/200 g BW/day), P2 (high-fat diet for 14 days, followed by standard feed, distilled water and bilimbi fruit and turmeric extract at a dose of 360: 175 mg/200 g BW/day), P3 (high-fat diet for 14 days, followed by standard feed, distilled water and extracts of bilimbi fruit and turmeric at a dose of 120: 405 mg/200 g BW/day). The determination of LDL levels was done through a direct method using a spectrophotometer with a wavelength of 550 nm.
Results: The mean LDL levels were KN (53.60 ± 10.69) mg/dL, K- (193.40 ± 16.86) mg/dL, K+ (100.20 ± 42.48) mg/dL, P1 (118.00 ± 64.71) mg/dL, P2 (104.00 ± 45.28) mg/dL, P3 (78.00 ± 4.69) mg/dL. The data received was analyzed using the Kruskal-Wallis test. Then, the results obtained differences in LDL levels between various groups (p<0.001). Subsequently, it was continued with the Mann-Whitney test on KN compared to K-, K+, P1, P2, P3 indicating p=0.009. As a result, it was found a significant difference in LDL levels between KN and K-, K+, P1, P2, and P3.
Conclusions: This research concludes that providing a mixture of bilimbi fruit extract and turmeric extract can reduce LDL levels.
Sendy, A. M. dan Widodo, A. Pengaruh Ekstrak Daun Belimbing Wuluh (Averrhoa Blimbi L.)Terhadap Kadar Kolesterol LDL Serum Tikus Wistar (Rattus Norvegicus) Hiperkolesterolemia. Diponegoro Med. J. (Jurnal Kedokt. Diponegoro) 8 (2), 642–652 (2019).
Lawuningtyas, A. et al. Hubungan Tingkat Pengetahuan dan Ketepatan Penggunaan Obat Simvastatin Correlation between Hypercholesterolemic Patient ’ s Knowledge and Simvastatin Use in Malang Retail Pharmacies. Pharm. J. Indones. 5 (2), 91–96 (2020).
Perkeni. Panduan Pengelolaan Dislipidemia Di Indonesia 2021. (2021).
Marco Sentosa, Tyas Rini Saraswati, S. T. Kadar Low Density Lipoprotein ( LDL ) Kuning Telur Puyuh Jepang ( Coturnix coturnix japonica L .) setelah Pemberian Tepung Kunyit ( Curcuma Longa L .) pada Pakan Low Density Lipoprotein ( LDL ). Bul. Anat. dan Fisiol. 2 (1), (2017).
Fauziah, F., Uthia, R. dan Musdar, M. Pengaruh Ekstrak Etanol Daun Belimbing Wuluh ( Averrhoa Bilimbi L .) Terhadap Kadar Kolesterol Total Dan LDL Pada Mencit Putih Jantan Hiperkolesterolemia. J. Farm. Higea 10 (2), 116–125 (2018).
Fairudz, A. & Nisa, K. Pengaruh serat pangan terhadap kadar kolesterol penderita overweight. Med. J. Lampung Univ. 4, 121–126 (2015).
Putri, N. A. dan Sri Gumilar, M. Efek Hipolipidemik Alpukat (Persea Americana) Pada Hiperkolesterolemia: Literatur Review. J. Bahana Kesehat. Masy. (Bahana J. Public Heal. 3 (2), 108–114 (2019).
Riskesdas. Riset Kesehatan Dasar 2013. (2013).
Laporan Nasional Riskesdas. Laporan Nasional Riset Kesehatan Dasar 2018. REPOSITORI BADAN LITBANG KESEHATAN RI 198 (2018).
Laporan Nasional Riskesdas. Laporan_Nasional_RKD2018_FINAL.pdf. Badan Riset dan Pengembangan Kesehatan 198 (2018).
Rosyida, R., Yuniarti, Y., Mintarsih, S. N., Ambarwati, R. & Larasati, M. D. Efektifitas Pemberian Jus Jambu Biji Terhadap Kadar Kolesterol Total Pada Pralansia Overweight. J. Ris. Gizi 6, 31 (2018).
Pratiwi, H., Winarso, D. dan Handoyo, N. Effect of Turmeric Etanol Extract (Curcuma Longa L) on Low Density Lipoprotein Level and Liver Histopathology Image in Type 1 Diabetes Mellitus Rat Model Induced by Streptozotocin. J. Ilmu dan Teknol. Has. Ternak 11 (1), 1–7 (2016).
Doja, B. C. D., Rame, M. M. T. dan Rengga, M. P. E. Uji Aktivitas Antihiperkolesterolemia Ekstrak Metanol Buah Belimbing Wuluh (Averrhoa Bilimbi Linn.) Pada Tikus Putih (Rattus Norvegicus) Galur Wistar. CHMK Pharm. Sci. J. 3 (2), 3 (2020).
Nurbaiti, Satriansyah, M. F. dan Gustine, R. (4), pp. 263–269. Nurbaiti, Satriansyah, M. F. dan Gustine, R. 2017. Efektivitas Ekstrak Belimbing Wuluh ( Avorrhea Belimbi L. ) Terhadap Kadar Kolesterol Total, High-Density Lipoprotein ( HDL ), dan Low-Density Lipoprotein ( LDL ) pada Tikus Putih Hiperk. J. Kedokt. dan Kesehat. 3 (1), 28–38 (2017).
Othman, F. A. et al. Toxicity Evaluation of A. Bilimbi L. Fruit Extract on Haematological and Histopathological Analysis in Animal Model. Pharm J. 39–43 (2019).
Baratzadeh, F., Butler, A. dan Kesharwani, P. Effects Of Curcumin On Low-Density Lipoprotein Oxidation: From Experimental Studies To Clinical Practice. EXCLI J. 840–851 (2022) doi:https://doi.org/10.17179/excli2022-4878.
Copyright (c) 2024 Amerta Nutrition
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
AMERTA NUTR by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under "the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.
