The Impact of Dadih Functional Bread on Gestational Weight Gain for Pregnant Women in Padang, Indonesia: a Randomized Controlled Trial
Pengaruh Roti Fungsional Dadih terhadap Kenaikan Berat Badan Ibu Hamil di Padang, Indonesia: a Randomized Controlled Trial

Background: Gestational Weight Gain (GWG) is correlated to the growth and development of foetus and pregnancy outcomes. Probiotics have a positive effect on gestational weight gain. Dadih is a traditional West Sumatra food containing 108 CFU/ml of probiotics.
Objectives: This research aimed to investigate the effect of dadih functional bread on gestational weight gain.
Methods: This research is a Randomised Controlled Trials (RCT) design with double blinds with a gestational age of 12-20 weeks belonging to 88 pregnant women. The tests for weight balancing scales were used. The data were examined using Independent T-test for difference test and General Liniar Model (GLM) repeated measures, and Analysis of Covariance (ANCOVA).
Results: The average GWG was significantly 2 kg greater for the intervention group related to the control group (7.3±2.6 kg vs 5.4±3.8 kg). The GWG in the intervention group exceeded the recommended GWG per month. The results indicated that weight gain was difference across the four-time measurements in both groups. There was a significant different in the average GWG among the intervention control and the control group (p-value<0.001).
Conclusions: Dadih functional bread supplementation for three months significantly effect the gestational weight gain. Thus, the consumption of the dadih products can be considered when planning the nutrition to advance the nutritional status of pregnant women.
Lipoeto NI, Wattanapenpaiboon N, Malik A, Wahlqvist BMedSc ML. The nutrition transition in West Sumatra, Indonesia. Asia Pac J Clin Nutr 2004; 13: 312–316.
Aji AS, Lipoeto NI, Yusrawati Y, Malik SG, Kusmayanti NA, Susanto I et al. Association between pre-pregnancy body mass index and gestational weight gain on pregnancy outcomes: a cohort study in Indonesian pregnant women. BMC Pregnancy Childbirth 2022; 22: 492. https://doi.org/10.1186/s12884-022-04815-8.
Dewey KG. Formulations for Fortified Complementary Foods and Supplements: Review of Successful Products for Improving the Nutritional Status of Infants and Young Children. Food Nutr Bull 2009; 30: S239–S255. https://doi.org/10.1177/15648265090302S209.
Ministry of Health of the Republic of Indonesia. Basic Health Research 2018. Jakarta, 2018.
Ministry of Health of the Republic of Indonesia. Basic Health Research 2013. Jakarta, 2013.
Gusnedi G, Fahmida U, Witjaksono F, Nurwidya F, Mansyur M, Djuwita R et al. Effectiveness of optimized food-based recommendation promotion to improve nutritional status and lipid profiles among Minangkabau women with dyslipidemia: A cluster-randomized trial. BMC Public Health 2022; 22: 1–12. https://doi.org/10.1186/S12889-021-12462-5/TABLES/4.
Rahmah H (Hasnaini), Nurlinda A (Andi), Kurnaesih E (Een). The Effect of Supplementary Feeding on Body Weight of Pregnant Women Who Have Chronic Energy Deficiency in Indonesia. Journal of Aafiyah Health Research 2022; 3: 44–51. https://doi.org/10.52103/JAHR.V3I1.947.
Soltani H, Lipoeto NI, Fair FJ, Kilner K, Yusrawati Y. Pre-pregnancy body mass index and gestational weight gain and their effects on pregnancy and birth outcomes: a cohort study in West Sumatra, Indonesia. BMC Womens Health 2017; 17: 102. https://doi.org/10.1186/s12905-017-0455-2.
Viswanathan M, Siega-Riz AM, Moos MK, Deierlein A, Mumford S, Knaack J et al. Outcomes of maternal weight gain. Evid Rep Technol Assess (Full Rep) 2008; : 1.
Halkjær SI, de Knegt VE, Lo B, Nilas L, Cortes D, Pedersen AE et al. Multistrain Probiotic Increases the Gut Microbiota Diversity in Obese Pregnant Women: Results from a Randomized, Double-Blind Placebo-Controlled Study. Curr Dev Nutr 2020; 4: nzaa095. https://doi.org/10.1093/cdn/nzaa095.
Dhillon P, Singh K, Kaur K. The benefits of probiotic interventions in maternal-fetal health: An appraise review. PharmaNutrition 2020; 13: 100211. https://doi.org/10.1016/j.phanu.2020.100211.
Sánchez B, Delgado S, Blanco‐Míguez A, Lourenço A, Gueimonde M, Margolles A. Probiotics, gut microbiota, and their influence on host health and disease. Mol Nutr Food Res 2017; 61. https://doi.org/10.1002/mnfr.201600240.
Turjeman S, Collado MC, Koren O. The gut microbiome in pregnancy and pregnancy complications. Curr Opin Endocr Metab Res 2021; 18: 133–138. https://doi.org/10.1016/J.COEMR.2021.03.004.
Santacruz A, Collado MC, García-Valdés L, Segura MT, Martín-Lagos JA, Anjos T et al. Gut microbiota composition is associated with body weight, weight gain and biochemical parameters in pregnant women. British Journal of Nutrition 2010; 104: 83–92. https://doi.org/10.1017/S0007114510000176.
Santacruz A, Collado MC, García-Valdés L, Segura MT, Martín-Lagos JA, Anjos T et al. Gut microbiota composition is associated with body weight, weight gain and biochemical parameters in pregnant women. British Journal of Nutrition 2010; 104: 83–92. https://doi.org/10.1017/S0007114510000176.
Surono IS. Traditional Indonesian dairy foods. Asia Pac J Clin Nutr 2015; 24: 26–30. https://doi.org/10.6133/apjcn.2015.24.s1.05.
Putra AA (A), Marlida Y (Y), Khasrad K (Khasrad), Azhike SY (S), Wulandari R (R). Perkembangan dan Usaha Pengembangan Dadih: sebuah Review Tentang Susu Fermentasi Tradisional Minangkabau. Jurnal Peternakan Indonesia 2011; 13: 159–170. https://doi.org/10.25077/JPI.13.3.159-170.2011.
Surono IS, Martono PD, Kameo S, Suradji EW, Koyama H. Effect of probiotic L. plantarum IS-10506 and zinc supplementation on humoral immune response and zinc status of Indonesian pre-school children. Journal of Trace Elements in Medicine and Biology 2014; 28: 465–469. https://doi.org/10.1016/j.jtemb.2014.07.009.
Hartriyanti Y, Suyoto PS, Muhammad HF, Palupi IR. Nutrient Intake of Pregnant Women in Indonesia: A Review. Mal J Nutr 2012; 18: 113–124.
Choi H, Lim J-Y, Lim N-K, Ryu HM, Kwak DW, Chung JH et al. Impact of pre-pregnancy body mass index and gestational weight gain on the risk of maternal and infant pregnancy complications in Korean women. Int J Obes 2022; 46: 59–67. https://doi.org/10.1038/s41366-021-00946-8.
Tela FG, Bezabih AM, Adhanu AK. Effect of pregnancy weight gain on infant birth weight among mothers attending antenatal care from private clinics in Mekelle City, Northern Ethiopia: A facility based follow-up study. PLoS One 2019; 14: e0212424. https://doi.org/10.1371/journal.pone.0212424.
Moll U, Olsson H, Landin-Olsson M. Impact of Pregestational Weight and Weight Gain during Pregnancy on Long-Term Risk for Diseases. PLoS One 2017; 12: e0168543. https://doi.org/10.1371/journal.pone.0168543.
Venema K, Surono IS. Microbiota composition of Dadih - a traditional fermented buffalo milk of West Sumatra. Lett Appl Microbiol 2019; 68: 234–240. https://doi.org/10.1111/lam.13107.
Taufiqa Z, Chandra DN, Helmizar H, Lipoeto NI, Hegar B. Micronutrient Content and Total Lactic Acid Bacteria of Dadiah Pudding as Food Supplementation for Pregnant Women. Open Access Maced J Med Sci 2021; 9: 1149–1155. https://doi.org/10.3889/oamjms.2021.6791.
Balqis R, Putra AE, Utama BI, Helmizar H. Pengaruh Pemberian Dadih dengan Perubahan Jumlah Lactobacillus fermentum Pada Feses Ibu Hamil. Jurnal Kesehatan Andalas 2018; 7: 42. https://doi.org/10.25077/jka.v7i0.872.
Celi P, Cowieson AJ, Fru-Nji F, Steinert RE, Kluenter A-M, Verlhac V. Gastrointestinal functionality in animal nutrition and health: New opportunities for sustainable animal production. Anim Feed Sci Technol 2017; 234: 88–100. https://doi.org/10.1016/j.anifeedsci.2017.09.012.
Collado MC, Surono IS, Meriluoto J, Salminen S. Potential Probiotic Characteristics of Lactobacillus and Enterococcus Strains Isolated from Traditional Dadih Fermented Milk against Pathogen Intestinal Colonization. J Food Prot 2007; 70: 700–705. https://doi.org/10.4315/0362-028X-70.3.700.
Sánchez B, Delgado S, Blanco‐Míguez A, Lourenço A, Gueimonde M, Margolles A. Probiotics, gut microbiota, and their influence on host health and disease. Mol Nutr Food Res 2017; 61. https://doi.org/10.1002/mnfr.201600240.
Judkins TC, Archer DL, Kramer DC, Solch RJ. Probiotics, Nutrition, and the Small Intestine. Curr Gastroenterol Rep 2020; 22: 2. https://doi.org/10.1007/s11894-019-0740-3.
Soltani H, Fair F, Marvin-Dowle K, Lipoeto NI. The effects of a local dairy food supplement on pregnancy outcomes in Indonesia: A feasibility trial. Eur J Public Health 2020; 30. https://doi.org/10.1093/eurpub/ckaa166.924.
Ferraro ZM, Barrowman N, Prud’homme D, Walker M, Wen SW, Rodger M et al. Excessive gestational weight gain predicts large for gestational age neonates independent of maternal body mass index. The Journal of Maternal-Fetal & Neonatal Medicine 2012; 25: 538–542. https://doi.org/10.3109/14767058.2011.638953.
Akgun N, Keskin HL, Ustuner I, Pekcan G, Avsar AF. Factors affecting pregnancy weight gain and relationships with maternal/fetal outcomes in Turkey. Saudi Med J 2017; 38: 503–508. https://doi.org/10.15537/smj.2017.5.19378.
Rasmussen KM, Yaktine AL. Weight Gain During Pregnancy: Reexamining the Guidelines. Food and Nutrition Board, Board on Children, Youth and Families, Institute of Medicine, National Research Council: Weight Gain During Pregnancy: Reexamining the Guidelines 2009; : 71–110.
Sahin ME, Madendag IC. Effect of Gestational Weight Gain on Perinatal Outcomes in Low Risk Pregnancies with Normal Prepregnancy Body Mass Index. Biomed Res Int 2019; 2019: 1–4. https://doi.org/10.1155/2019/3768601.
Sunsaneevitayakul P, Sompagdee N, Dehghan MA-, Talungchit P. Effect of Gestational Weight Gain on Overweight and Obese Pregnant Women. Siriraj Med J 2022; 74: 364–370. https://doi.org/10.33192/Smj.2022.44.
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