Most Probable Number of Escherichia Coli in Fresh Milk at KPSP Ijen Makmur, Licin Sub-District, Banyuwangi
Downloads
The study aimed to identify the total number of E. coli used in fresh cow milk in KPSP Ijen Makmur. The sample was used by as many as 16 samples from a group of cattle farmers. E. coli analyzed using Most Probable Number (MPN), 3 series of tubes. Before continuing the MPN test the milk must be diluted, 25 ml of milk was poured into the dilution of the 225ml peptone water buffered then homogenized for 2 minutes. MPN consist of presumptive coliform test if positive was found of gas and was cloudy, presumptive faecal coliform test positive was found in the gas and was cloudy, confirmed E.coli test if there was a black colony with or metallic green, continued by a biochemical test with red ring-positive Indole, Methyl Red positive the color is red, negative Voges Paskauer if there is no change in color, and negative citrate will turn green. Results showed that from the 16 samples of fresh milk used there were 7 samples of fresh milk that exceeded the contamination limit E. coli or < 3 apm /ml. Conclusion, number of E. coli in fresh milk at KPSP Ijen Makmur 43.75% of the total sample exceeded maximum contamination.
Bahri, S. (2008). Beberapa aspek keamanan pangan asal ternak di Indonesia. Pengembangan Inovasi Pertanian, 1(3), 225.
Balia, R. L., Harlia, E & Suryanto, D. (2008). Jumlah bakteri total dan koliform pada susu segar peternakan sapi perah rakyat dan susu pasteurisasi tanpa kemasan di pedagang kaki lima. Prosiding Prospek Industri Sapi Perah Menuju Perdagangan Bebas, 2020, pp.322-325.
Budiyanto, A., & Usmiati, S. (2008). Pemerahan susu secara higienis menggunakan alat perah sederhana. In Seminar Nasional Teknologi Peternakan dan Veteriner (pp. 327-334).
Gustiani, E. (2009). Pengendalian cemaran mikroba pada bahan pangan asal ternak (daging dan susu) mulai dari peternakan sampai dihidangkan. Jurnal Litbang Pertanian, 28(3), 96-100.
Handayani, K. S., & Maya, P. (2010). Kesehatan ambing dan higien pemerahan di peternakan sapi perah desa pasir buncir kecamatan caringin. Jurnal Penyuluhan Peternakan, 5(1).
Hijriah, P. F., Santoso, P. E., & Wanniatie, V. 2016. Status mikrobiologi (total plate count, coliform, dan Escherichia coli) susu kambing peranakan etawa (pe) di desa Sungai Langka kecamatan Gedong Tataan Kabupaten Pesawaran. Jurnal Ilmiah Peternakan Terpadu, 4(3), 217-221.
Jawetz, E., J. Melnick, & E. Adelberg. (2013). Medical Microbiology. 26th Edition. Mc Graw Hill. 232-235.
Jeffrey, T., Lejeune, & P.J.R. Schultz. (2009). Unpasteurized milk: A continued public health threat. Food Safety. Clinical Infectious Dis, (48), 93-100.
Maulida, F.N. (2013). Tatalaksana Kesehatan Peternakan Sapi Perah Rakyat di Kecamatan Cisarua Kabupaten Bogor. Skripsi. Bogor: Fakultas Kedokteran Hewan Institut Pertanian Bogor. 6-26.
Musawir, M. A., & Arsin, A. A. (2016). Kontaminasi bakteri Escherichia coli pada botol susu dengan kejadian diare pada bayi. Media Kesehatan Masyarakat Indonesia, 10(3), 146-153.
Permatasari, R. I. (2018). Hygiene, Sanitation and Bacteriological Quality of Cow's Milk in Krajan Design, Gendro Village, Tutur Regency of Pasuruan District. Jurnal Kesehatan Lingkungan, 10(4), 343-350.
Pradika, A. Y., Chusniati, S., Purnama, M. T. E., Effendi, M. H., Yudhana, A. & Wibawati, P. A. (2019). Uji Total E. coli pada Susu Sapi Segar di Koperasi Peternak Sapi Perah (KPSP) Karyo Ngremboko Kecamatan Purwoharjo Kabupaten Banyuwangi. Jurnal Medik Veteriner, 2(1), 1-6.
Prihutomo, S., Setiani, B. E., & Harjanti, D. W. (2015). Screening sumber cemaran bakteri pada kegiatan pemerahan susu di peternakan sapi perah rakyat Kabupaten Semarang. Jurnal Ilmu-Ilmu Peternakan, 25(1), 66-71.
Saleh, E. (2004). Teknologi Pengolahan Susu dan Hasil Ikutan Ternak, Progam Studi Produksi Ternak, Fakultas Pertanian Universitas, Sumatera Utara, Digitized by USU digital library. 9(2), 77-85.
Sartika, R. A. D., Indrawani, Y. M., & Sudiarti, T. (2005). Analisis Mikrobiologi E. coli O157:H7 Pada Hasil Olahan Hewan Sapi Dalam Proses Produksinya. Makara Kesehatan, 9(1), 27.
SNI 01-2332.1. (2015). Cara Uji Mikrobiologi - Bagian 1: Penentuan Coliform Dan E. coli Pada Produk Perikanan. Badan Standarisasi Nasional Indonesia.
SNI 3141. (2011). Susu Sapi Segar. Badan Standarisasi Nasional Indonesia.
SNI 7388. (2009). Batas Maksimum Cemaran Bakteri dalam Pangan. Badan Standarisasi Nasional Indonesia.
Sutiknowati, L. I. (2016). Bioindikator Pencemar, Bakteri E. coli. Jurnal Oseana, 41(4), 63-71.
Suwito, W. (2016). Bakteri yang sering mencemari susu: deteksi, patogenesis, epidemiologi, dan cara pengendaliannya. Jurnal Penelitian dan Pengembangan Pertanian, 29(3), 96-100.
Vimont, A., Vernozy‐Rozand, C., & Delignette‐Muller, M. L. (2006). Isolation of E. coli O157: H7 and non‐O157 STEC in different matrices: review of the most commonly used enrichment protocols. Letters in applied microbiology, 42(2), 102-108.
Wijiastutik, D. (2012). Hubungan Higiene dan Sanitasi Pemerahan Susu Sapi dengan Total Plate Count pada Susu Sapi di Peternakan Sapi Perah Manggis Kabupaten Boyolali. Jurnal Kesehatan Masyarakat Volume 1 Nomer 2 Tahun 2012. Universitas Diponegoro.
Authors who publish in this journal agree to the following terms:
1. The journal allows the author to hold the copyright of the article without restrictions;
2. The journal allows the author(s) to retain publishing rights without restrictions;
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (CC BY-NC-SA).
11.jpg)
