Efectiveness of Ocimum sanctum Linn. Leaves Ethanol Extract on Mortality Raillietina echinobothrida In Vitro

Jessica Giovani, Setiawan Koesdarto, Mirny Lamid, Sri Agus Sudjarwo, Muchammad Yunus, Suryo Kuncorojakti

= http://dx.doi.org/10.20473/jops.v2i1.16383
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Abstract


The aims of the research are to prove the anthelmintic effects of Ocimum sanctum Linn. leaves ethanol extract on Raillietina echinobothrida in vitro and to know the value of LC50 and LC90of Ocimum sanctum Linn. leaves ethanol extract. The research used completely randomized design. The research had five treatments and each treatment was done in four replications. The research used 5 Raillietina echinobothrida adult worms for each treatment and all replications. The observation and the recording were done at the first hour until the sixth hour of the research. The Raillietina echinobothrida adult worms were noted dead if there was no movement when they were disturbed by anatomic tweezer, the death of Raillietina echinobothrida were confirmed when they dipped mildly in warm water (50°C) and they showed no movements. The acquired data was analyzed using ANOVA and continued with LSD (5%). The results were Ocimum santum Linn. ethanol extract has the anthelmintic activity in 1.25%, 2.5%, 5% and 10% concentrations. The LC50 and LC90 was analyzed using probit analysis. The results of LC50 calculation were 7.8% at the third hour, 6% at the forth hour, 3.2% at the fifth hour, 2.5% at the sixth hour and the results of LC90 were 6.5% at the fifth hour, 5.2% at the sixth hour. The higher concentration of the extract, the higher anthelmintic activity worked.


Keywords


Ocimum sanctum Linn. leaves, ethanol extract, Raillietina echinobothrida, in vitro

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References


Asha, M. K., D. Parasanth, B. Murali, R. Padmaja and A. Amit. 2001. Anthelmintic Activity of Essential Oil of Ocimum sanctum and euge-nol. Elsevier Science B.V.: Fitoterapia 72 (2001) 669-­‐670.

Athanasiadou, S., I. Kyriazakis, F. Jackson, and R. L. Coop. 2001. Direct anthelmintic effects of condensed tannins towards different gastro-in¬testinal nematodes of sheep in vitro and in vivo studies. Vet. Parasitol. 99, 205–219.

Hoste, H., J. Frank, A. Spiridoula, M. T. Stig and O. H. Simone. 2006. The Effects of Tannin rich Plants on Parasitic Nematodes in Rumi¬nants. Science Direct J. Trends in Parasito¬logy, Vol. 22, No. 6.

Hussain, A. 2008. Evaluation of Anthelmintic Activity of Some Ethnobotanicals. Faculty of Veterinary Science. University of Agricul-ture Faisalabad Pakistan.

Jagadeesan, K., T. George, M. N. Kamalanathan, and T. Ilyaraja 2014. Studies on the Anthel-mintic and Antioxidant Activity of Root Ext-racts of Elytratria Acaulis. Research of Ani-mal and Veterinary Sciences, Depart¬me¬nt¬ of Zoology, President College, Vol. 2, Issue 8, Page 457, 2014.


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