Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas
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This study aimed to determine the effect of Moringa (Moringa oleifera Lam.) leaf extract on the viability and motility of spermatozoa of rats (Rattus norvegicus) exposed to heat (40 ºC). A total of 25 rats aged 2.5 months and weighed 200 grams were randomly divided into five groups. Rats in K (–) group were not exposed to heat and without Moringa leaf extract administration. Rats in K (+) group were exposed to heat (40 ºC) for 1 hour daily without Moringa leaf extract administration. Meanwhile, rats in P1, P2, and P3 groups were administrated with Moringa leaf extract 100, 200, and 400 mg/kg BW/day, then exposed to 40 ºC 1 hour daily for 14 days. The results showed that exposure to 40 °C one hour per day for 14 days in K (+) group caused a decrease (p <0.05) on viability and motility of rat spermatozoa compared to those of the K (–) group. The administration of Moringa leaf extract (P1, P2, and P3 groups) was followed by an increase (p <0.05) in viability and motility of rat spermatozoa compared to the K (+) group. Viability and motility of rat spermatozoa in the P3 group (given Moringa leaf extract 400 mg/kg BW/day and exposed to 40 ºC 1 hour daily for 14 days) were not significantly different (p >0.05) compared to the viability and motility of rat spermatozoa in K (–) group. It could be concluded that administration of Moringa leaf extract 400 mg/kg BW/day can maintain the viability and motility of spermatozoa in white rats exposed to heat.
Agati G, Brunetti C, Di Ferdinando M, Ferrini F, Pollastri S, Tattini M. 2013. Functional roles of flavonoids in photoprotection: new evidence, lessons from the past. Plant Physiol Biochem. 72: 35-45.
Agati G, Brunetti C, Fini A, Gori A, Guidi L, Landi M, Sebastiani F, Tattini M. 2020. Are Flavonoids Effective Antioxidants in Plants? Twenty Years of Our Investigation. Antioxidants (Basel) 9: 1098.
Alahmar AT. 2019. Role of Oxidative Stress in Male Infertility: An Updated Review. J Hum Reprod Sci. 12: 4-18.
Andani ND, Sasmito B, Hani'ah H. 2018. Pengaruh Perubahan Tutupan Lahan Terhadap Fenomena Urban Heat Island dan Keterkaitannya dengan tingkat Kenyamanan Termal (Temperature Humidity Index) di Kota semarang. J Geodesi Undip. 7: 53-65.
Arifin B, Ibrahim S. 2018. Struktur, Bioaktivitas dan Antioksidan Flavonoid. J Zarah. 6: 21-9.
Badriyah B, Achmadi J, Nuswantara LK. 2017. Kelarutan Senyawa Fenolik dan Aktivitas Antioksidan Daun Kelor (Moringa Olifera Lam) di dalam Rumen Secara In Vitro. J Peternakan Indonesia. 19: 116-21.
Bardaweel SK, Gul M, Alzweiri M, Ishaqat A, ALSalamat HA, Bashatwah RM. 2018. Reactive Oxygen Species: the Dual Role in Physiological and Pathological Conditions of the Human Body. Eurasian J Med. 50:193-201.
Cahyadi TRT, Christiyanto M, Setiatin ET. 2016. Persentase hidup dan abnormalitas sel spermatozoa kambing Peranakan Etawah (PE) dengan pakan yang disuplementasi Daun Binahong (Anredera cordifolia (Ten.) Steenis). Anim Agric J. 5: 23-32.
Das AK, Rajkumar V, Verma AK, Swarup D. 2012. Moringa oleifera leaves Extract: A Natural Antioxidant for Retarding Lipid Peroxidation in Cooked Goat Meat Patties. J Food Sci Tech. 47: 585-91.
de La Salles AYF, Batista LF, de Souza BB, da Silva AF, de Barros Correia EL. 2017. Growth and reproduction hormones of ruminants subjected to heat stress. J Anim Behav Biometeorol. 5: 7-12.
de los Reyes M, Palomino J, Martínez V, Aretio C, Gutiérrez M. 2011. Acrosin release and acrosin activity during incubation in capacitating media using fresh and frozen-thawed dog sperm. Biol Res. 44:139-44.
Durairajanayagam D, Agarwal A, Ong C, Prashast P. 2014. Lycopene and male infertility. Asian J Androl. 16: 420-5.
El-Tantawy WH. 2015. Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats. J Tradit Complement Med. 6: 327-31.
Ermiza E. 2012. Pengaruh paparan suhu terhadap kualitas spermatozoa mencit jantan (Mus musculus) Strain Jepang. Sainstis 1: 19-28.
Estrada E, Rodríguez-Gil JE, Rivera Del Álamo MM, Peña A, Yeste M. 2017. Effects of reduced glutathione on acrosin activity in frozen-thawed boar spermatozoa. Reprod Fertil Dev. 29: 283-93.
Handayani E, Supriatna I, Tumbelaka LI, Kaiin EM. 2021. Analisis Komparatif Kualitas Semen Beku yang Telah dan Belum Bersertifikasi Standar Nasional Indonesia. J Vet. 22: 207-15.
Lamando D, Soegianto A, Abadi A, Keman S. 2014. Antioxidant effects of sarang semut (Myrmecodia pendans) on the apoptosis of spermatogenic cels of rats exposed to plumbum. J Pharm Biol Chem Sci. 5: 282-94.
Margaritelis NV, Cobley JN, Paschalis V, Veskoukis AS, Theodorou AA, Kyparos A, Nikolaidis MG. 2016. Going retro: Oxidative stress biomarkers in modern redox biology. Free Radic Biol Med. 98: 2-12.
Novita M, Sulaiman MI, Yura S. 2016. Pengaruh Jenis Pelarut Aktivitas Antioksidan dan Kandungan Fenol Beberapa Jenis Bayam dan Sayuran Lain. J Ilmiah Mahasiswa Pertanian Unsyiah 1: 935-40.
Ogunsola OA, Owalabi JO, Fabiyi OS, Nwobi NL, Faluyi B, Akinbola AS. 2017. Moringa Plant Consumption had Effects on Reproduction Functions in Male and Female Rat Models. J Dent Med Sci. 16: 82-6.
Panggalih A, Susilowati S, Maslachah L, Ratnani H, Suprayogi TW. 2021. The effect of watermelon (Citrullus lanatus) rind ethanolic extract on the number of Leydig, Sertoli, and spermatogenic cells of rat (Rattus novergicus) exposed to heat. Ovozoa 10: 7-11.
Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. 2017. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev. 2017:8416763.
Prastyaningtyas K, Kurnijasanti R, Sugihartuti R, Susilowati S, Suprayogi TW, Eliyani H. 2021. The effect of red dragon fruit (Hylocereus polyrhizus) peel extract on Leydig cells number, seminiferous tubules diameter, and testicular weight of mice (Mus musculus) exposed to heat. Ovozoa 10: 18-24.
Pratama R. 2019. Efek rumah kaca terhadap bumi. Buletin Utama Teknik 14: 120-26.
Pratiwi IN, Aligita W, Kaniawati M. 2021. Kajian potensi antioksidan dari tanaman herbal dan pengaruhnya terhadap penyakit Parkinson. J Ilmiah Farmasi 17: 80-95.
Raharjo R, Sudjarwo SA, I'tishom R. 2017. Effects of Red Dragon Fruit (Hylocereus polyrhizus) skin extract on lead acetate toxicity in the morphology of Balb/c MICE (Mus musculus) spermatozoa. Folia Medica Indonesiana 53: 237-41.
Riesco MF, Alvarez M, Anel-Lopez L, Neila-Montero M, Palacin-Martinez C, Montes-Garrido R, Boixo JC, de Paz P, Anel L. 2021. Multiparametric Study of Antioxidant Effect on Ram Sperm Cryopreservation-from Field Trials to Research Bench. Animals 11: 283.
Rohmah L, Triana IN, Sunarso A, Susilowati S, Hidajati N, Kurnijasanti R. 2018. Pengaruh pemberian ekstrak kulit semangka (Citrullus lanatus) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan suhu panas. Ovozoa 7: 131- 6.
Sabés-Alsina M, Tallo-Parra O, Mogas MT, Morrell JM, Lopez-Bejar M. 2016. Heat stress has an effect on motility and metabolic activity of rabbit spermatozoa. Anim Reprod Sci. 173:18-23.
Sarfraz A, Qureshi AA, Shahid RU, Hussain M, Usman M, Umar Z. 2019. Maintenance of Thermal Homeostasis with Special Emphasis on Testicular Thermoregulation." Acta Vet Eurasia 45: 63.
Silva LKX, Sousa JS, Silva AOA, Lourenço Junior JB, Faturi C, Martorano LG, Franco IM, Pantoja MHA, Barros DV, Garcia AR. 2018. Testicular thermoregulation, scrotal surface temperature patterns and semen quality of water buffalo bulls reared in a tropical climate. Andrologia 50: 11-8.
Solihati N, Purwantara B, Supriatna I, Winarto A. 2013. Perkembangan sel-sel spermatogenik dan kualitas sperma pascapemberian ekstrak pegagan (Centella asiatica). Indones J Anim Vet Sci. 18: 192-201.
Subianto A, Boer R, Aldrian E, Perdinan P dan Kinseng R. 2018. Isu Perubahan Iklim dalam konteks Keamanan Nasional. J Ketahanan Nas. 24: 287-305.
Tavalaee M, Sadeghi N, Nasr-Esfahani MH. 2018. Effect of heat stress on Spermatogenesis. J Shahrekord Univ Med Sci. 20: 110-27.
Utami W, Mardawati E, Putri SH. 2020. Pengujian aktivitas antioksidan kulit Buah Naga Merah (Hylocereus polyrhizus) sebagai masker gel peel off. J Industri Pertanian 2: 95-102.
Vadnais ML, Cao W, Aghajanian HK, Haig-Ladewig L, Lin AM, Al-Alao O, Gerton GL. 2014. Adenine nucleotide metabolism and a role for AMP in modulating flagellar waveforms in mouse sperm. Biol Reprod. 90: 128.
Wagner H, Cheng JW, Ko EY. 2017. Role of reactive oxygen species in male infertility: An updated review of literature. Arab J Urol. 16: 35-43.
Widiantoro K, Madyawati SP, Sardjito T, Hernawati T, Triana IN, Warsito SH. 2021. Kualitas post-thawing semen domba Merino dalam bahan pengencer berbasis susu skim-kuning telur yang ditambah isolat crude protein Tirosine Kinase. Ovozoa 10: 39-45
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