Antibiotic sensitivity test of Escherichia coli and Staphylococcus aureus isolated from the reproductive tract of dairy cows
Downloads
The purpose of this study was to determine the sensitivity of Staphylococcus aureus and Escherichia coli isolated from the reproductive tract of dairy cows during estrus to tetracycline, gentamicin, amoxicillin, and ciprofloxacin. Isolation and identification of E. coli and S. aureus from 24 cervical mucus samples resulted 15 samples containing E. coli and one sample containing S. aureus. Sensitivity test was performed using Kirby-Bauer (disc diffusion) method and interpreted using the Clinical and Laboratory Standards Institute (CLSI, 2017) guidelines. The sensitivity test of 15 isolates of E. coli showed 100% sensitivity to ciprofloxacin and tetracycline. For gentamicin, 14 (93.3%) isolates were sensitive, and 1 (6.7%) isolate was resistant, while for amoxicillin, 14 (93.3%) isolates were sensitive, and 1 (6.7%) isolate were intermediate. The sensitivity test of one isolate of S. aureus showed 100% sensitivity to ciprofloxacin, gentamicin, and amoxicillin; as for tetracycline 100% isolates were declared resistant.
Aditi FY, Rahman SS, Hossain MM. 2017. A Study on the Microbiological Status of Mineral Drinking Water. Open Microbiol J. 11: 31-44.
Agga GE, Arthur TM, Durso LM, Harhay DM, and Schmidt JW. 2015. Antimicrobial-resistant bacterial populations and antimicrobial resistance genes obtained from environments impacted by livestock and municipal waste. PLoS One 10: e0132586.
Amin YA, Ali RA, Fouad SS, Ibrahim RM. 2021. The deleterious effect of postpartum pyometra on the reproductive indices, the metabolic profile, and oxidant/antioxidant parameters of dairy cows. Vet World. 14: 329-38.
Appiah MO, Wang J, Lu W. 2020. Microflora in the Reproductive Tract of Cattle: A Review (Running Title: The Microflora and Bovine Reproductive Tract). Agriculture 10: 232.
Bambang AG, Fatimawati, Kojong NS. 2014. Analisis cemaran bakteri coliform dan identifikasi E. coli pada air isi ulang dari depot di Kota Manado. J ilmiah Farmasi UNSRAT. 3: 325-34.
Bhat FA, Bhattacharyya HK, Hussain SA. 2014. White side test: A simple and rapid test for evaluation of nonspecific bacterial genital infections of repeat breeding cattle. Vet Res Forum 5: 177-80.
Buckley F, Lopez-Villalobos N, Heins BJ. 2014. Crossbreeding: implications for dairy cow fertility and survival. Animal 8: 122-33.
Bush K, Bradford PA.2016. β-Lactams and β-Lactamase Inhibitors: An Overview. Cold Spring Harb Perspect Med. 6: a025247.
Campos CC, do Prado FL, dos Reis JPJ, Carneiro LC, Silva PRB, Moraes GF, Santos RM. 2020. Effects of clinical mastitis and puerperal diseases on reproductive efficiency of dairy cows. Trop Anim Health Prod. 52: 3061-8.
Compton CWR, Heuer C, Thomsen PT, Carpenter TE, Phyn CVC, McDougall S. 2017. Invited review: A systematic literature review and meta-analysis of mortality and culling in dairy cattle. J Dairy Sci. 100: 1-16.
Dewi AK. 2013. Isolasi, Identifikasi dan Uji Sensitivitas Staphylococcus aureus terhadap Amoxicillin dari Sampel Susu Kambing Peranakan Ettawa (PE) Penderita Mastitis di Wilayah Girimulyo, Kulonprogo, Yogyakarta. J Sain Vet. 31: 140-1.
Effendi MH, Piesha D, Galiono D. 2013. Resistensi Staphylococcus Koagulase Positif terhadap Antibiotik Non-Beta Laktam Isolat dari Kasus Pyoderma pada Anjing. Veterinaria Medika 6: 55-9.
Froböse NJ, Bjedov S, Schuler F, Kahl BC, Kampmeier S, Schaumburg F. 2020. Gram Staining: a Comparison of Two Automated Systems and Manual Staining. J Clin Microbiol. 58: e01914-20.
Garneau-Tsodikova S, Labby KJ. 2016. Mechanisms of Resistance to Aminoglycoside Antibiotics: Overview and Perspectives. Medchemcomm. 7: 11-27.
Grossman TH. 2016. Tetracycline Antibiotics and Resistance. Cold Spring Harb Perspect Med. 6: a025387.
Hananto W, Rudyanto MD, and Suardana IW. 2015. Isolasi dan Identifikasi Escherichia coli O157:H7 pada Sapi Bali di Kuta Selatan, Badung, Bali. Indonesia Medicus Veterinus 4: 351-61.
Hangas A, Aasumets K, Kekäläinen NJ, Paloheinä M, Pohjoismäki JL, Gerhold JM, Goffart S. 2018. Ciprofloxacin impairs mitochondrial DNA replication initiation through inhibition of Topoisomerase 2. Nucleic Acids Res. 46: 9625-36.
Isnan MH, Gelgel KTP and Suarjana IGK. 2017. Isolasi dan Identifikasi Bakteri dari Susu Kambing Peranakan Etawa Terindikasi Mastitis Klinis di Beberapa Kecamatan di Kabupaten Banyuwangi. Buletin Veteriner Udayana 9: 73-80.
Kaur SP, Rao R, Nanda S. 2011. Amoxicillin: A Broad-Spectrum Antibiotic. Int J Pharm Sci. 3: 30-7.
Kumar M, Pant SS, Ram R, Kumar S, Gupta PK. 2014. Therapeutic Efficacy of Levofloxacin along with Vitamin E for the Management of Repeat Breeding Syndrome in Cow under Field Condition. Inter J Vet Sci. 3: 155-7.
Kustanti NOA. 2016. Efisiensi Reproduksi Sapi Perah Friesian Holstein (Studi kasus Di Peternakan Bapak Nur Trianto Desa Ngaglik Kecamatan Srengat Kabupaten Blitar). AVES: Jurnal Ilmu Peternakan 10: 35-42.
Leboffe MJ, Pierce BE. 2011. A Photographic Atlas for the Microbiology Laboratory. 4th Ed. Morton Publishing, Colorado.
Leininger DJ, Roberson JR, Elvinger F. 2001. Use of eosin methylene blue agar to differentiate Escherichia coli from other gram-negative mastitis pathogens. J Vet Diagn Invest. 13: 273-5.
Mathew B, Agrawal S, Nashikkar N, BundaleS, Upadhyay A. 2019. Isolation of Acetic Acid Bacteria and Preparation of Starter Culture for Apple Cider Vinegar Fermentation. Adv Microbiol. 9: 556-69.
Molina-Coto R, Lucy MC. 2018. Uterine inflammation affects the reproductive performance of dairy cows: A review, Agronomía Mesoamericana 29: 449-68.
Mustafa HSI. 2014. Staphylococcus aureus Can Produce Catalase Enzyme When Adding to Human WBCs as a Source of H2O2 Productions in Human Plasma or Serum in the Laboratory. Open J Med Microbiol. 4: 249-51.
Nur MO, Mulyati S, Chusniati S, Sardjito T, Tyasningsih W, Mafruchati M. 2020. Profil bakteri non spesifik dalam lendir serviks sapi perah pada fase folikuler dan fase luteal. Ovozoa 9: 17-22.
Öztürkler Y, Ucar O. 2006. Treatment of Infertile Cows: Efficacy of PGF2α and Gentamicin or Their Combination in a PRID Regime. Turk J Vet Anim Sci. 30: 521-25.
Pohl A, Burfeind O, Heuwieser W. 2015. The associations between postpartum serum haptoglobin concentration and metabolic status, calving difficulties, retained fetal membranes, and metritis. J Dairy Sci. 98: 4544-51.
Prendiville R, Pierce KM, Delaby L, Buckley F. 2011. Animal performance and production efficiencies of Holstein-Friesian, Jersey and Jersey í— Holstein-Friesian cows throughout lactation. Livest Sci. 138: 25-33.
Qomariyah AA, Indrayudha P. 2021. Antibacterial Activity of Combination of Ethanol Extract of Pepermine Leaves (Mentha piperita L.) and Amikacin Against Klebsiella pneumonia, Staphylococcus aureus, and Escherichia coli. J Nutraceuticals Herbal Medicine 4: 12-29.
Rahayu SA, Gumilar MMH. 2017. Uji Cemaran Air Minum Masyarakat Sekitar Margahayu Raya Bandung Dengan Identifikasi Bakteri Escherichia coli. Indo J Pharm Sci Tech. 4: 50-56.
Ribeiro ES, Gomes G, Greco LF, Cerri RLA, Vieira-Neto A, Monteiro Jr PLJ, Lima FS, Bisinotto RS, Thatcher WW, Santos JEP. 2016. Carryover effect of postpartum inflammatory diseases on developmental biology and fertility in lactating dairy cows. J Dairy Sci. 99: 2201-20.
Rosadi B, Sumarsono T, Hoesni F. 2018. Identifikasi Gangguan Reproduksi pada Ovarium Sapi Potong yang Mengalami Anestrus Postpartum Panjang. J Vet. 1: 385-89.
Saimin J, Hartati H, Purnamasari Y, Mulyawati SA, Tien T, Aritrina P. 2020. Microbiological and Biochemical Contamination Analysis of Refilled Drinking-water in Abeli, Kendari, Southeast Sulawes. Indones Biomed J. 12: 124-9.
Shakya P, Barrett P, Diwan V, Marothi Y, Shah H, Chhari N, Tamhankar AJ, Pathak A, Lundborg CS. 2013. Antibiotic resistance among Escherichia coli isolates from stool samples of children aged 3 to 14 years from Ujjain, India. BMC Infect Dis. 13: 477.
Sharma S, Singh M, and Vasishta NK. 2009. Isolation and antimicrobial susceptibility of aerobic bacteria recovered from the uteri of dairy cows suffering from endometritis. Indian J Anim Sci. 79: 278-82.
Sudrajad K, Madyawati SP, Tyasningsih W, Srianto P, Widodo OS. 2018. Bacterial isolates from the cervical mucus of dairy cattle at follicular and luteal phases. The Philipp J Vet Med. 55: 121-26.
Todar K. 2002. Antimicrobial Agents Used in the Treatment of Infectious Disease. Textbook of Bacteriology, University of Wisconsin-Madison.
Toelle NN, Lenda V. 2014. Identifikasi dan Karakteristik Staphylococcus Sp. dan Streptococcus Sp. dari Infeksi Ovarium Pada Ayam Petelur Komersial. J Ilmu Ternak 1: 32-7.
Trzcinski K, Cooper BS, Hryniewicz W, Dowson CG. 2000. Expression of resistance to tetracyclines in strains of methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother. 45: 763-70.
Ullah R, Qureshi AW, Sajid A, Khan I, Ullah A, Taj R. 2021. Percentage Incidences of Bacteria in Mahseer (Tor putitora), Silver carp (Hypophthalmichthys molitrix) Fish Collected from Hatcheries and Local Markets of District Malakand and Peshawar of Khyber Pakhtunkhwa, Pakistan. Braz J Biol. 84: 1-6.
Utami RE. 2011. Antibiotika, Resistensi, dan Rasionalitas Terapi. El-Hayah.1: 191-8.
Copyright (c) 2022 Baswendra Triadi, Suwarno Suwarno, Suryanie Sarudji, Ratna Damayanti, Rahmi Sugihartuti, Agnes Theresia Soelih Estoepangesti
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Ovozoa by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under "the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.
