Potential of Basil (Ocimum basilicum) leaf extract in Tris-egg yolk extender on sperm motility and plasma membrane integrity of Sapudi ram at room temperature
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This study aimed to determine the effect of basil (Ocimum basilicum) leaf extract in a Tris-egg yolk extender on the motility, and plasma membrane integrity of Sapudi ram spermatozoa at room temperature. Fresh semen samples were collected from Sapudi ram using an artificial vagina. A completely randomized design was used, consisting of four treatment groups with five replicates each. Data were analyzed using SPPS with factorial analysis of variance (ANOVA) and Duncan’s test. The control group (T0) received Tris-egg yolk extender without basil leaf extract. Treatment groups T1, T2, and T3 received 1%, 2%, and 3% basil leaf extract, respectively, in the extender. Results showed that sperm motility and plasma membrane integrity (Table 4) tended to decrease with increased storage time. Among the treatment groups, the addition of 1% basil leaf extract in the Tris-egg yolk extender (T1) resulted in significantly higher (p <0.05) both sperm motility and plasma membrane integrity compared to the control (T0). However, higher concentrations of basil leaf extract (T2 and T3) did not result in significant differences (p >0.05) in sperm motility after three hours of storage compared to the 1% treatment (T1). In conclusion, the addition of 1% basil leaf extract to a Tris-egg yolk extender was effective in maintaining sperm motility and plasma membrane integrity of Sapudi ram spermatozoa stored at room temperature for up to three hours.
A'yuni DQ, Sa'adi A, Widjiati W. 2023. Ethanol extract of basil (Ocimum basilicum L.) leaves inhibits endometriosis growth in a mouse model by modulating vascular endothelial growth factor (VEGF) expression. J Med Life 16: 1224-30.
Bahadi MA, Al-Badwi MA, Samara EM, Abdoun KA, Alhidary IA, Al-Haidary AA. 2023. Group-training of rams at puberty for artificial vagina-mediated semen collection and its influence on semen quality and sexual behavior. Anim Reprod. 20: e20220051.
Chang F, Zhang B, Liu H, Fan H, Xie R, Li J, Hu Q, Ruan C. 2025. Effect of centrifuged chicken egg yolk on the cryopreservation of boar semen. Animals (Basel) 15: 599.
Gibbons AE, Fernandez J, Bruno-Galarraga MM, Spinelli MV, Cueto MI. 2019. Anim Reprod. 16: 803-809.
Gulcin İ. 2025. Antioxidants: a comprehensive review. Arch Toxicol. 99: 1893-997.
Gündoğan M, Tekerli M, Uçar M, Türkmenoğlu I. 2003. Effect of diluents on motility of ram sperm during storage at 5°C. J Reprod Sys. 49: 69-75.
Halaweh W, Khnissi S, Ben Souf I, Salman M, M'Hamdi N. 2025. Impact of mating methods and semen preservation on reproductive and growth performances in Palestinian Assaf sheep. Biology (Basel). 14: 80.
Hassanpour SH, Doroudi A. 2023. Review of the antioxidant potential of flavonoids as a subgroup of polyphenols and partial substitute for synthetic antioxidants. Avicenna J Phytomed. 13: 354-76.
Ibrahim A, Baliarti E, Budisatria IGS, Artama WT, Widayanti R, Maharani D, Tavares L, Margawati ET. 2023. Genetic diversity and relationship among Indonesian local sheep breed on Java Island based on mitochondrial cytochrome b gene sequences. J Genet Eng Biotechnol. 21: 34.
Juárez-Rojas L, Casillas F, López A, Betancourt M, Ommati MM, Retana-Márquez S. 2022. Physiological role of reactive oxygen species in testis and epididymal spermatozoa. Andrologia 54: e14367.
Kowalczyk A. 2022. The role of the natural antioxidant mechanism in sperm cells. Reprod Sci. 29: 1387-94.
Liang J, Lv C, Yang H, Zhang Y, Raza SHA, Wu G, Quan G. 2022. Ultrastructural modification of ram sperm frozen with cyclohexanediol and trehalose. Biopreserv Biobank 20: 348-56.
Madrigali A, Rota A, Panzani D, Castellani S, Shawahina M, Hassan A, Di Iacovo F, Rossignoli C, Camillo F. 2021. Artificial insemination in sheep with fresh diluted semen Trop Anim Sci J. 44: 255-60.
Maia MdS, Bicudo SD, Azevedo HC, Sicherle CC, de Sousa DB, Rodello L. 2009. Motility and viability of ram sperm cryopreserved in a Tris-egg yolk extender supplemented with anti-oxidants. Small Rumin Res. 85: 85-90.
Miranda AS, Suprayogi TW, Utomo B, Susilowati S, Dhamayanti Y. 2023. Impact of green tea (Camellia sinensis) leaf extract in skim milk-goose egg yolk semen extender on the quality of Sapudi sheep spermatozoa stored at 5°C. Ovozoa: J Anim Reprod. 12: 148-56.
Mishra R, Nikam A, Hiwarkar J, Nandgude T, Bayas J, Polshettiwar S. 2024. Flavonoids as potential therapeutics in male reproductive disorders. Futur J Pharm Sci. 10: 100.
Muarifah H, Maharani D, Bintara S, Budisatria IGS. 2019. Growth performance on Sapudi ewe’s birth type in Sapudi Island, Madura, East Java, Indonesia. The UGM Annual Scientific Conference Life Sciences 2016. KnE Life Sci. 174-8.
Nadeem HR, Akhtar S, Sestili P, Ismail T, Neugart S, Qamar M, Esatbeyoglu T. 2022. Toxicity, antioxidant activity, and phytochemicals of Basil (Ocimum basilicum L.) leaves cultivated in Southern Punjab, Pakistan. Foods 11: 1239.
Palacin I, Santolaria P, Alquezar-Baeta C, Soler C, Silvestre MA, Yániz J. 2020. Relationship of sperm plasma membrane and acrosomal integrities with sperm morphometry in Bos taurus. Asian J Androl. 22: 578-82.
Paul RK, Kumar D, Naqvi S. 2017. Antioxidants protect proteins' anchorage to the bilayer by improving plasma membrane integrity of ram spermatozoa during liquid preservation in a soya lecithin-based diluent. Reprod Domest Anim. 52: 1052-60.
Purdy PH, Mocé E, Stobart R, Murdoch WJ, Moss GE, Larson B, Ramsey S, Graham JK, Blackburn HD. 2010. The fertility of ram sperm held for 24h at 5 degrees C prior to cryopreservation. Anim Reprod Sci. 118: 231-5.
Quraini SP, Susilowati S, Restiadi TI, Chusniati S. 2022. Comparison of different poultry egg yolks-citrate extender with green tea (Camellia sinensis) extract addition on Sapudi ram spermatozoa quality in chilled temperature storage. Ovozoa: J Anim Reprod. 11: 93-7.
Rajabi-Toustani R, Mehr MR, Motamedi-Mojdehi R. 2021. Reduction of seminal plasma concentration can decrease detrimental effects of seminal plasma on chilled ram spermatozoa. Anim Reprod. 18: e20200211.
Rather HA, Islam R, Malik AA, Lone FA. 2016. Addition of antioxidants improves quality of ram spermatozoa during preservation at 4°C. Small Rumin Res. 141: 24-8.
Romano R, De Luca L, Aiello A, Pagano R, Di Pierro P, Pizzolongo F, Masi P. 2022. Basil (Ocimum basilicum L.) leaves as a source of bioactive compounds. Foods 11: 3212.
Sari RP, Nugraha CD, Suyadi S. 2024. Effect of thawing on semen quality of Boer goats with Tris-aminomethane diluent supplemented with antioxidant Quercetin. J Sain Peternakan Indonesia 19: 11-20,
Sigit B, Maharani D, Tavares L, Iustitia SP. 2024. Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram. J Anim Sci Technol. 66: 891-904.
Sodiq A, Tawfik ES. 2004. Productivity and breeding strategies of sheep in Indonesia: A review. J Agric Rural Dev Trop Subtrop. 105: 71-82.
Spencer JP, Vauzour D, Rendeiro C. 2009. Flavonoids and cognition: the molecular mechanisms underlying their behavioural effects. Arch Biochem Biophys. 492: 1-9.
Sujarwanta RO, Afidah U, Suryanto E, Rusman, Triyannanto E, Hoffman LC. 2024. Review: Goat and sheep meat production in Indonesia. Sustainability 16: 4448.
Tapia JA, Macias-Garcia B, Miro-Moran A, Ortega-Ferrusola C, Salido GM, Peña FJ, Aparicio IM. 2012. The membrane of the mammalian spermatozoa: much more than an inert envelope. Reprod Domest Anim. 47: 65-75.
Tateishi R, Ogawa-Kishida N, Fujii N, Nagata Y, Ohtsubo Y, Sasaki S, Takashima K, Kaneko T, Higashitani A. 2024. Increase of secondary metabolites in sweet basil (Ocimum basilicum L.) leaves by exposure to N2O5 with plasma technology. Sci Rep. 14: 12759.
Triyaningrum T, Fahrudin M, Karja NWK, Prasetyaningtyas WE. 2024. The quality of chilled ram semen after storage in extender with addition of melatonin at 4° C. J Kedokteran Hewan 18: 34-9.
Van de Hoek M, Rickard JP, de Graaf SP. 2022. Motility assessment of ram spermatozoa. Biology (Basel) 11: 1715.
Wang Y, Fu X, Li H. 2025. Mechanisms of oxidative stress-induced sperm dysfunction. Front Endocrinol (Lausanne) 16: 1520835.
Zahra M, Abrahamse H, George BP. 2024. Flavonoids: Antioxidant powerhouses and their role in nanomedicine. Antioxidants (Basel) 13: 922.
Zaini BSI, Pujiana IM, Djamaludin EO, Salim HM. 2020. The effectiveness of extract Basil leaves on motility and morphology of spermatozoa mice exposed with cigarette smoke. Biomol Health Sci J. 3: 76-80.
Zhang L, Wang X, Sohail T, Jiang C, Sun Y, Wang J, Sun X, Li Y. 2024. Punicalagin protects ram sperm from oxidative stress by enhancing antioxidant capacity and mitochondrial potential during liquid storage at 4 °C. Animals (Basel) 14: 318.
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