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Pengaruh Induksi Bakteri Pseudomonas aeruginosa Terhadap Human Umbilical Vein Endothelial Cells (HUVECS) Culture
[ The Influence Of Pseudomonas aeruginosa Induction To The Human Umbilical Vein Endothelial Cells (HUVECS) Culture ]
Corresponding Author(s) : Dwi Yuni Nur Hidayati
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 1 No. 1 (2009): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
One of the cause of negative gram bacteria is Pseudomonas aeruginosa (Ps. aeruginosa). It continuing become sepsis on the first step of patogenesa happen sticking between aeruginosa with endhotel cell. This sticking is mediated by adhesi molecul which have characterize same with hemaglutin protein. The main study of this research is to know the profile of endothelial cell culture within induction of Ps. aeruginosa. The methods are Ps. aeruginosa (9064) which have been isolated then processes on TCBS Media and continued with isolation terracely. The profile of weight molecule protein obtained by SDS PAGE and then conducted electroelution. The test of hemaglutinin using eritrosit mencit. The receptor cell used is endhotel cell (HUVECs) culture. The result show that dosage of hemaglutin protein of Aeruginosa bring effect to profile of HUVECs culture determined by index adhesion of Ps. aeruginosa to HUVECs culture. We conclude that protein 38,19 kDa give effect to profile of HUVECs culture in vitro.
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- Castric Peter, Cassels Frederick J.,Carlson Russel W, 2001, Sructural Charcterization of the Pseudomonas aeruginosa 1244 Pilin Glycan, Journal of Biological Chemistry, 270 ( 28) p 26479 – 26485
- Comer Jason E,Marshal Mark A, Blanch Vincent J , Deal Carolyn D., 2002, Identifification of the Pseudomonas aeruginosa 1244 Pilin glycosylation Site, Infection and Immunity, 70 ( 6) p 2837 – 2845
- Comolli Janes C., Waite Leslie L., Mostov Keith E., Engel Joanne N., 1999, Pili Bending to Asialo – GM 1 on Epithelial Cells Can Mediate Cytotoxicity or Bacterial Internalisation by Pseudomonas aeruginosa, Infection and Imunity 67 (7) p 3207 – 3214
- Jun Lin-Tong, Garduno Rafael, Boudreau Robert T.M., Issekutz Andrew C., 2002, Pseudomonas aeruginosa Activates Human Mast Cells to Induce Neutrophil Transendothelial Migration Via Mast Cell-Derived IL -1 and 1 , Journal Immunology, 169: 4522 -4530
- Nagayama, K., Oguchi,T., Arita, M., Honda,T.,1995, Purification and Characterization of A Cell Associated Haemagglutinin of Vibrio parahaemolyticus, Infec.and Immun 63 (5): 1987 – 1992
- Salyers Abigail A., Whitt Dixie D., 1994. Bacterial Pathogenesis. Washington DC: ASM Press, p.31 -35 ; 262
- Stathopoulos Christos, Hendrixson David R. Thanassi David G., Hultgreen Scott J.,2000, Secretion of Virulence determinants by the general secretory pathway in Gram – negative pathogens : an evolving story, Microbes and infection,2, p 1061 – 1072
- Virella Gabriel, 1997. Gram – negative Rods III : Opportunistic and Zoonotic Bacteri ; Microbiology and infectious disease 3 rd edition, Philadelpia : Williams & Willins awaverly Company,p.159 – 162
- Wizzemann, T.M., Adamoum J.E.,and Langermann, S., 1999, Adhesins as Targets for Vaccine Development, Centers for Disease Control and Prevevtion, USA, p.1-5
References
Castric Peter, Cassels Frederick J.,Carlson Russel W, 2001, Sructural Charcterization of the Pseudomonas aeruginosa 1244 Pilin Glycan, Journal of Biological Chemistry, 270 ( 28) p 26479 – 26485
Comer Jason E,Marshal Mark A, Blanch Vincent J , Deal Carolyn D., 2002, Identifification of the Pseudomonas aeruginosa 1244 Pilin glycosylation Site, Infection and Immunity, 70 ( 6) p 2837 – 2845
Comolli Janes C., Waite Leslie L., Mostov Keith E., Engel Joanne N., 1999, Pili Bending to Asialo – GM 1 on Epithelial Cells Can Mediate Cytotoxicity or Bacterial Internalisation by Pseudomonas aeruginosa, Infection and Imunity 67 (7) p 3207 – 3214
Jun Lin-Tong, Garduno Rafael, Boudreau Robert T.M., Issekutz Andrew C., 2002, Pseudomonas aeruginosa Activates Human Mast Cells to Induce Neutrophil Transendothelial Migration Via Mast Cell-Derived IL -1 and 1 , Journal Immunology, 169: 4522 -4530
Nagayama, K., Oguchi,T., Arita, M., Honda,T.,1995, Purification and Characterization of A Cell Associated Haemagglutinin of Vibrio parahaemolyticus, Infec.and Immun 63 (5): 1987 – 1992
Salyers Abigail A., Whitt Dixie D., 1994. Bacterial Pathogenesis. Washington DC: ASM Press, p.31 -35 ; 262
Stathopoulos Christos, Hendrixson David R. Thanassi David G., Hultgreen Scott J.,2000, Secretion of Virulence determinants by the general secretory pathway in Gram – negative pathogens : an evolving story, Microbes and infection,2, p 1061 – 1072
Virella Gabriel, 1997. Gram – negative Rods III : Opportunistic and Zoonotic Bacteri ; Microbiology and infectious disease 3 rd edition, Philadelpia : Williams & Willins awaverly Company,p.159 – 162
Wizzemann, T.M., Adamoum J.E.,and Langermann, S., 1999, Adhesins as Targets for Vaccine Development, Centers for Disease Control and Prevevtion, USA, p.1-5