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Fatty Acid Profiling of Moina sp. Preserved in Cryoprotective Agents at Low Temperature
Corresponding Author(s) : Dr. Nurul Ulfah Karim
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 13 No. 2 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Highlight Research
- Saturated fatty acid (SFA), polyunsaturated fatty acid (PUFA), ∑ ω6 and ∑ ω3 of Moina sp. preserved with 5, 10 and 20% GLY decreased with prolong storage.
- FA of Moina sp. preserved with 5, 10 and 20% EG showed a significant reduced only after M3.
- Monounsaturated fatty acid (MUFA) and PUFA of Moina sp. preserved with 5, 10 and 20% DMSO increased significantly (p<0.05) after M3.
- Moina sp. preserved in 5% DMSO maintain the docosaheaenoic acid (DHA) level, which could be potential method for long-term preservation.
Abstract
Moina sp. is an important feed for larval and post-larval rearing of aquaculture species. Preservation of Moina sp. using various preservation agents and techniques is known to be less time-consuming procedure in maintaining large number of feed in hatchery management. Hence, this study was carried out to determine the changes of fatty acid (FA) composition of Moina sp. preserved at 5, 10 and 20% in glycerol (GLY), ethylene glycol (EG) and dimethyl sulfoxide (DMSO) for 1 to 3 months (M1-M3). Moina sp. without cryoprotectant agents stored at -40°C as controls. Saturated fatty acid (SFA), polyunsaturated fatty acid (PUFA), ∑ ω6 and ∑ ω3 of Moina sp. preserved with 5, 10 and 20% GLY decreased with prolonging storage. FA of Moina sp. preserved with 5, 10 and 20% EG showed a significant reduction only after M3. Monounsaturated fatty acid (MUFA) and PUFA of Moina sp. preserved with 5, 10 and 20% DMSO increased significantly (p<0.05) after M3. Moina sp. preserved in 5% DMSO maintain the docosaheaenoic acid (DHA) level, which could be a potential method for long-term preservation.
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- Airina, N. M., & Jamaludin, M. (2012). Fatty acids composition of selected Malaysian fishes. Sains Malaysiana, 41(1):81-94.
- Akbary, P., Hosseini, S. A., & Imanpoor. (2011). M.R. Enrichment of Artemia nauplii with essential fatty acids and vitamin C: Effect on rainbow trout (Oncorhynchus mykiss) larvae performance. Iran Journal Fisheries Sciences, 10(4):557-569.
- Armitage, W. J., & Mazur, P. (1984). Toxic and osmotic effects of glycerol on human granulocytes. The American Journal of Physiology Cell, 247(5):382-389.
- Berz, D., McCormack, E. M., Winer, E. S., Colvin, G. A., & Quesenberry, P. J. (2007). Cryopreservation of hematopoietic stem cells. The American Journal of Hematology, 82:463-472.
- Bhattacharya, S. (2018). Cryoprotectants and their usage in cryopreservation process. In Y. Bozkurt (Ed.), Cryopreservation Biotechnology in Biomedical and Biological Sciences. (pp. 7-18). London: IntechOpen.
- Bordignon, F., Martinez-Llorens, S., Trocina, A., Jover-Cerda, M., & Tomas-Vidal, A. (2020). Recovery of fatty acid composition in Mediterranean Yellowtail (Seriola dumerili, Risso 1810) fed a fish-oil finishing diet. International Journal of Molecular Sciences, 21:4871-4889.
- Cavalli, R. O., Lavens, P., & Sorgeloos, P. (1999). Performance of Macrobrachium rosenbergii broodstock fed diets with different fatty acid composition. Aquaculture, 179:387-402.
- Chen, X., & Thibeault, S. (2013). Effect of DMSO concentration, cell density and needle gauge on the viability of cryopreserved cells in three dimensional hyaluronan hydrogel. Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 6228-6231.
- Costa, L., Ottoni, M. H. F., Dos Santos, M. S., Meireles, A. B., De Almeida, V. G., Pereira, W. F., De Avelar-Freitas, B. A., & Brito-Melo, G. E. A. (2017). Dimethyl sulfoxide (DMSO) decreases cell proliferation and TNF-α, IFN-γ, and IL-2 cytokines production in cultures of peripheral blood lymphocytes. Molecules (Basel, Switzerland), 22(11):1789.
- D'Abramo, L. R., & Sheen, S. S. (1993). Polyunsaturated fatty acid nutrition in juvenile freshwater prawn Macrobrachium rosenbergii. Aquaculture, 115:63-86.
- Das, P., Mandal, S. C., Bhagabati, S. K., Akhtar, M. S., & Singh, S. K. (2012). Important live food organisms and their role in aquaculture. In M. Sukham (Ed.), Frontiers in Aquaculture. (pp. 69-86). Mumbai: Narendra Publishing House.
- Davies, O. G., Smith, A. J., Cooper, P. R., Shelton, R. M., & Scheven, B. A. (2014). The effects of cryopreservation on cells isolated from adipose, bone marrow and dental pulp tissues. Cryobiology, 69:342-347.
- Hubel, A. (1997). Parameters of cell freezing: implications for the cryopreservation of stem cells. Transfusion Medicine Review, 11:224-233.
- Jang, T. H., Park, S. C., Yang, J. H., Kim, J. Y., Seok, J. H., Park, U. S., Choi, C. W., Lee, S. R., & Han, J. (2017). Cryopreservation and its clinical application. Integrative Medicine Research, 6:12-18.
- Kangpanish, C. (2016). Effects of alternative oil sources, n- ton-6 ratios and arachidonic acid supplementation in feed on growth and reproduction of giant freshwater prawn. Thesis. Thailand: Burapha University.
- Loh, J. Y., Ong, H. K., Hii, Y. S., Smith, T. J., Lock, M. W., & Khoo, G. (2012). Highly unsaturated fatty acid (HUFA) retention in the freshwater cladoceran, Moina macrocopa enriched with lipid emulsions. Israeli Journal of Aquaculture, 4:637-646.
- Majid, M. I. A., Endarti, E., Hashim, R., & Chong, A. (2003). Direct FAME synthesis for rapid total lipid analysis from fish oil and cod liver oil. Journal of Food Composition and Analysis, 18:161-171.
- Mohammad, S. N., & Mustafa, S. (2012). Makanan hidup untuk ikan. Johor: Dewan Bahasa dan Pustaka.
- Mubarak, A. S., Jusaidi, D., Zarin, M. Jr., & Suprayudi, M. A. (2017). Evaluation of the rice brand and cassava suspension use in the production of male Moina offspring and ephippia. Aquaculture Aquarium Conservation and Legislation, 10(3):512-524.
- Regulska, M., Pomierny, B., Basta-Kaim, A., Starek, A., Fili, M., Lason, W., & Budziszewska, B. (2010). Effects of ethylene glycol ethers on cell viability in the human neuroblastoma SH-SY5Y cell line. Pharmacological Report, 62(6):1242-1239.
- Srivastava, P. K. (2012). Short term preservation of Moina micrura; Influence of storage on protein, lipid and carbohydrate contents. Trend in Life Sciences, 1(1):1-5.
- Tuschong, L., Soenen, S. L., Blaese, R. M., Candotti, F., & Muul, L. M. (2002). Immune response to fetal calf serum by two adenosine deaminase-deficient patients after T cell gene therapy. Human Gene Therapy, 13:1605-1610.
- Yan, L. J. (2011). Fatty acid enrichment and potential food source for Moina macrocopa cultivation. Thesis. Malaysia: Universiti Tunku Abdul Rahman.
- Yong, K. W., Pingguan-Murphy, B., Xu, F., Wan Abas, W. A. B., Choi, J. R., Omar, S. Z., Azmi, M. A. N., & Wan Safwani, W. K. Z. (2015). Phenotypic and functional cauterization of long-term cryopreserved human adipose-derived stem cells. Science Report, 5:1-10.
References
Airina, N. M., & Jamaludin, M. (2012). Fatty acids composition of selected Malaysian fishes. Sains Malaysiana, 41(1):81-94.
Akbary, P., Hosseini, S. A., & Imanpoor. (2011). M.R. Enrichment of Artemia nauplii with essential fatty acids and vitamin C: Effect on rainbow trout (Oncorhynchus mykiss) larvae performance. Iran Journal Fisheries Sciences, 10(4):557-569.
Armitage, W. J., & Mazur, P. (1984). Toxic and osmotic effects of glycerol on human granulocytes. The American Journal of Physiology Cell, 247(5):382-389.
Berz, D., McCormack, E. M., Winer, E. S., Colvin, G. A., & Quesenberry, P. J. (2007). Cryopreservation of hematopoietic stem cells. The American Journal of Hematology, 82:463-472.
Bhattacharya, S. (2018). Cryoprotectants and their usage in cryopreservation process. In Y. Bozkurt (Ed.), Cryopreservation Biotechnology in Biomedical and Biological Sciences. (pp. 7-18). London: IntechOpen.
Bordignon, F., Martinez-Llorens, S., Trocina, A., Jover-Cerda, M., & Tomas-Vidal, A. (2020). Recovery of fatty acid composition in Mediterranean Yellowtail (Seriola dumerili, Risso 1810) fed a fish-oil finishing diet. International Journal of Molecular Sciences, 21:4871-4889.
Cavalli, R. O., Lavens, P., & Sorgeloos, P. (1999). Performance of Macrobrachium rosenbergii broodstock fed diets with different fatty acid composition. Aquaculture, 179:387-402.
Chen, X., & Thibeault, S. (2013). Effect of DMSO concentration, cell density and needle gauge on the viability of cryopreserved cells in three dimensional hyaluronan hydrogel. Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 6228-6231.
Costa, L., Ottoni, M. H. F., Dos Santos, M. S., Meireles, A. B., De Almeida, V. G., Pereira, W. F., De Avelar-Freitas, B. A., & Brito-Melo, G. E. A. (2017). Dimethyl sulfoxide (DMSO) decreases cell proliferation and TNF-α, IFN-γ, and IL-2 cytokines production in cultures of peripheral blood lymphocytes. Molecules (Basel, Switzerland), 22(11):1789.
D'Abramo, L. R., & Sheen, S. S. (1993). Polyunsaturated fatty acid nutrition in juvenile freshwater prawn Macrobrachium rosenbergii. Aquaculture, 115:63-86.
Das, P., Mandal, S. C., Bhagabati, S. K., Akhtar, M. S., & Singh, S. K. (2012). Important live food organisms and their role in aquaculture. In M. Sukham (Ed.), Frontiers in Aquaculture. (pp. 69-86). Mumbai: Narendra Publishing House.
Davies, O. G., Smith, A. J., Cooper, P. R., Shelton, R. M., & Scheven, B. A. (2014). The effects of cryopreservation on cells isolated from adipose, bone marrow and dental pulp tissues. Cryobiology, 69:342-347.
Hubel, A. (1997). Parameters of cell freezing: implications for the cryopreservation of stem cells. Transfusion Medicine Review, 11:224-233.
Jang, T. H., Park, S. C., Yang, J. H., Kim, J. Y., Seok, J. H., Park, U. S., Choi, C. W., Lee, S. R., & Han, J. (2017). Cryopreservation and its clinical application. Integrative Medicine Research, 6:12-18.
Kangpanish, C. (2016). Effects of alternative oil sources, n- ton-6 ratios and arachidonic acid supplementation in feed on growth and reproduction of giant freshwater prawn. Thesis. Thailand: Burapha University.
Loh, J. Y., Ong, H. K., Hii, Y. S., Smith, T. J., Lock, M. W., & Khoo, G. (2012). Highly unsaturated fatty acid (HUFA) retention in the freshwater cladoceran, Moina macrocopa enriched with lipid emulsions. Israeli Journal of Aquaculture, 4:637-646.
Majid, M. I. A., Endarti, E., Hashim, R., & Chong, A. (2003). Direct FAME synthesis for rapid total lipid analysis from fish oil and cod liver oil. Journal of Food Composition and Analysis, 18:161-171.
Mohammad, S. N., & Mustafa, S. (2012). Makanan hidup untuk ikan. Johor: Dewan Bahasa dan Pustaka.
Mubarak, A. S., Jusaidi, D., Zarin, M. Jr., & Suprayudi, M. A. (2017). Evaluation of the rice brand and cassava suspension use in the production of male Moina offspring and ephippia. Aquaculture Aquarium Conservation and Legislation, 10(3):512-524.
Regulska, M., Pomierny, B., Basta-Kaim, A., Starek, A., Fili, M., Lason, W., & Budziszewska, B. (2010). Effects of ethylene glycol ethers on cell viability in the human neuroblastoma SH-SY5Y cell line. Pharmacological Report, 62(6):1242-1239.
Srivastava, P. K. (2012). Short term preservation of Moina micrura; Influence of storage on protein, lipid and carbohydrate contents. Trend in Life Sciences, 1(1):1-5.
Tuschong, L., Soenen, S. L., Blaese, R. M., Candotti, F., & Muul, L. M. (2002). Immune response to fetal calf serum by two adenosine deaminase-deficient patients after T cell gene therapy. Human Gene Therapy, 13:1605-1610.
Yan, L. J. (2011). Fatty acid enrichment and potential food source for Moina macrocopa cultivation. Thesis. Malaysia: Universiti Tunku Abdul Rahman.
Yong, K. W., Pingguan-Murphy, B., Xu, F., Wan Abas, W. A. B., Choi, J. R., Omar, S. Z., Azmi, M. A. N., & Wan Safwani, W. K. Z. (2015). Phenotypic and functional cauterization of long-term cryopreserved human adipose-derived stem cells. Science Report, 5:1-10.
Author Biography
Research Interests :
- Fisheries Post Harvest
- Seafood Safety and Quality
- Processing of Aquatic Products
- Microbiology