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Vol. 14 No. 1 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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November 20, 2021
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February 27, 2022

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Molecular Genetics Fish Diseases Fish Health Management

Immune Response of White Shrimp (Litopenaeus vannamei) to Different Density and IMNV Challenge

https://doi.org/10.20473/jipk.v14i1.31468
Nur Komariah Baladrat
Jakarta Technical University of Fisheries.
Moch Nurhudah
Polytechnic of Marine and Fisheries, Karawang.
Heny Budi Utari
Animal Health Service, Central Proteina Prima Company, Jakarta.

Corresponding Author(s) : Nur Komariah Baladrat

komariah535@gmail.com

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 14 No. 1 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Highlight Research

  1. White shrimp (Litopenaeus vannamei) challenged with IMNV and different stocking densities
  2. The water quality parameter values are getting worse along with the increase in stocking density
  3. White shrimp THC value decreased with increasing stocking density and IMNV challenge test
  4. The development of the IMNV increased with an increase in stocking density seen from the speed and percentage of the appearance of the clinical symptoms and histopathology results

 

Abstract

Increasing in stocking density of shrimp affects the physiology and behaviour of their moving space. The health condition of shrimp is influenced by feeding, growth, and its susceptibility on disease. The aim of this study was to determine the development of immune response in relation to density and the presence of IMNV infection. This study used a completely randomized design (CRD) at density of 100 shrimp.m-2, 200 shrimp.m-2, and 400 shrimp.m-2, with three replications in each treatment. The shrimp used was 5,02±0,26 g and the virus infection was exposed orally. This research was facilitated at the Disease Research Centre Laboratory of Central Proteina Prima Company, Pasar Kemis, Tangerang for 30 days. The results showed that the Total Hemocyte Count (THC) in hemolymph of shrimp had different values between negative controls and challenged IMNV. The lowest THC value was found at a density of 400 shrimp m-2 (3.00x106ml-1). While the highest THC value was at a density of 100 shrimp.m-2 (4.75x106ml-1). This result is supported by the increasing value of water quality parameters along with the increasing density of shrimp. Histopathology changes on skeletal muscle and lymphoid organs confirmed that the development of IMNV infection was faster at high shrimp densities.

Keywords

DensityTHCHistopathologyIMNV
Baladrat, N. K., Nurhudah, M., & Utari, H. B. (2022). Immune Response of White Shrimp (Litopenaeus vannamei) to Different Density and IMNV Challenge. Jurnal Ilmiah Perikanan Dan Kelautan, 14(1), 83–92. https://doi.org/10.20473/jipk.v14i1.31468
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References
  1. Aguilar, V., Racotta, I. S., Goytortua, E., WIlle, M., Sorgeloos, P., Civera, E., & Palacios, E. (2011). The influence of dietary arachidonic acid on teh immune response and perfomance of pacific whitelweg shrimp, Litopenaeus vannamei, at high stocking density. Aquaculture Nutrition, 18(3):258-271.
  2. Andrade, T. P. D., Redman, R. M., & Lightner, D. V. (2008). Evaluation of the preservation of shrimp samples with Davidson's AFA fixative for Infectious Myonecrosis Virus (IMNV) in situ hybridization. Aquaculture, 278(1-4):179-183.
  3. Apún-molina, J. P., Robles-romo, A., Alvarez-ruiz, P., Santamaria-miranda, A., Arjona, O., & Racotta, I. S. (2017). Influence of stocking density and exposure to White Spot Syndrome Virus in biological performance, metabolic, immune, and bioenergetics response of whiteleg shrimp Litopenaeus vannamei. Aquaculture, 479:528-537.
  4. Ariadi, H., Wafi, A., Magister, P., Brawijaya, U., Perikanan, P. B., Ibrahimy, U., & Brawijaya, U. (2020). Hubungan kualitas air dengan nilai FCR pada budidaya intensif udang vanname (Litopenaeus vannamei). Samakia: Jurnal Ilmu Perikanan, 11(1):44-50.
  5. Chang, C. F., Su, M. S., & Chen, H. Y. (1999). A rapid method to quantify total haemocyte count of Penaeus monodon using ATP analysis Fish Pathology, 34(4):211-212.
  7. FAO. (2016). The state of world fisheries and aquaculture. Rome: FAO.
  8. Fast, A. W., & Lester, L. J. (1992). Marine shrimp culture: Principles and practices. Amsterdam: Elsevier.
  9. Fendjalang, S. N. M., Budiardi, T., Supriyono, E. (2016). Kinerja produksi dan fisiologis udang vaname Litopenaeus vannamei pada karamba jaring apung dengan padat tebar berbeda di Selat Kepulauan Seribu. Thesis. Bogor: IPB University.
  10. Hasan, A. S. W. (2011). Ko-infeksi Infectious Myonecrosis Virus (IMNV) dan Vibrio harveyi pada udang vaname. Institut Pertanian Bogor. Thesis. Bogor: IPB University.
  11. Hendrawati, Prihadi, T. H., & Rohmah, N. N. (2008). Analisis kadar phosfat dan N-nitrogen (amonia, nitrat, nitrit) pada tambak air payau akibat rembesan lumpur lapindo di Sidoarjo, Jawa Timur. Jurnal Kimia Valensi, 1(3):135-143.
  12. Kurniawan, M. H., Putri, B., & Elisdiana, Y. (2018). Efektivitas pemberian bakteri Bacillus polymyxa melalui pakan terhadap imunitas non spesifik udang vannamei (Litopenaeus vannamei). E-Jurnal Rekayasa dan Teknologi Budidaya Perairan, VII(1):739-750.
  13. Kusumaningrum, E. D., Wardiyanto, & Tusihadi, T. (2012). Insidensi Infectious Myonecrosis Virus (IMNV) pada udang putih (Litopenaeus vannamei) di Teluk Lampung. E-Jurnal Rekayasa Dan Teknologi Budidaya Perairan, I(1):65-70.
  14. Li, Y., Li, J., & Wang, Q. (2006). The effects of dissolved oxygen concentration and stocking density on growth and non-specific immunity factors in Chinese shrimp, Fenneropenaeus chinensis. Aquaculture, 256(1-4):608-616.
  15. Lightner, D. V. (1996). Epizootiology, distribution and the impact on international trade of two penaeid shrimp viruses in the Americas. Scientific and Technical Review, 15(2):579-601.
  16. Lin, Y. C., Chen, J. C., Chen, Y. Y., Yeh, S. T., Chen, L. L., Huang, C. L., Hsieh, J. F., & Li, C. C. (2015). Crowding of white shrimp Litopenaeus vananmei depresses their immunity to and resistance against Vibrio alginolyticus and White Spot Syndrome Virus. Fish and Shellfish Immunology, 45(1):104-111.
  17. Miranti, S. (2016). Pengendalian infeksi Vibrio harveyi pada udang vaname dengan ekstrak kunyit-sambiloto dalam pakan di karamba jaring apung Kepulauan Seribu. Thesis. Bogor: IPB University.
  18. Molina, J. P. A., Romo, A. R., Ruiz, P. A., Miranda, A. S., Arjona, O., & Racotta, I. S. (2017). Influence of stocking density and exposure to White Spot Syndrome Virus in biological performance, metabolic, immune, and bioenergetics response of whiteleg shrimp Litopenaeus vannamei. Aquaculture, 479:528-537.
  19. Muharrama, A. R. W. (2020). Pertumbuhan, ekspresi gen dan respons imunitas udang vaname diberi probiotik Bacillus NP5 dan prebiotik madu serta diinfeksi Vibrio parahaemolyticus. Thesis. Bogor: IPB University.
  20. Poersch, L. H., Bauer, W., Wallner, M., & Wasielesky, W. (2020). Assessment of trace metals , total organic carbon and total nitrogen of a shrimp farm system in Southern Brazil. Regional Studies in Marine Science, 39:101452.
  21. Poulos, B. T., Tang, K. F. J., Pantoja, C. R., Bonami, J. R., & Lightner, D. V. (2006). Purification and characterization of Infectious Myonecrosis Virus of penaeid shrimp. Journal of General Virology, 87(4):987-996.
  22. Purnamasari, I., Purnama, D., & Utami, M. A. F. (2017). Pertumbuhan udang vaname (Litopenaus vannamei). Jurnal Enggano, 2(1):58-67.
  23. Ridlo, A., & Pramesti, R. (2009). Aplikasi ekstrak rumput laut sebagai agen imunostimulan sistem pertahanan non spesifik pada udang (Litopennaeus vannamei). Jurnal Ilmu Kelautan, 14:133-137.
  24. Rusaini, & Owens, L. (2010). Insight into the lymphoid organ of penaeid prawns: A review. Fish and Shellfish Immunology, 29(3):367-377.
  25. Sansanayuth, P., Phadungchep, A., Ngammontha, S., Ngdngam, S., Sukasem, P., Hoshino, H., & Ttabucanon, M. S. (1996). Shrimp pond effluent: Pollution problems and treatment by construted Wetlands. Water Science and Technology, 34(11):93-98.
  26. Sarah, H., Prayitno, S. B., & Haditomo, A. H. C. (2018). Studi kasus keberadaan penyakit IMNV (Infectious Myo Necrosis Virus) pada udang vaname (Litopenaeus vannamei) di pertambakan Pekalongan Jawa Tengah. Jurnal Sains Akuakultur Tropis, 2(1):66-72.
  27. Senapin, S., Phewsaiya, K., Briggs, M., & Flegel, T. W. (2007). Outbreaks of Infectious Myonecrosis Virus (IMNV) in Indonesia confirmed by genome sequencing and use of an alternative RT-PCR detection method. Aquaculture, 266(1-4):32-38.
  28. Silva, S. M. B. C. D., Rocha, J. L., Martins, P. C. C., Gálvez, A. O., Santos, F. L. D., Andrade, H. A., & Coimbra, M. R. M. (2015). Experimental infection of Infectious Myonecrosis Virus (IMNV) in the Pacific white shrimp Litopenaeus vannamei (Boone, 1931). Aquaculture International, 23:563-576.
  29. Song, Y. L., Yu, C. I., Lien, T. W., Huang, C. C., & Lin, M. N. (2003). Haemolymph parameters of Pacific white shrimp (Litopenaeus vannamei) infected with Taura Syndrome Virus. Fish & Shellfish Immunology, 14(4):317-331.
  30. Sookying, D., Silva, F. S. D., Davis, D. A., & Hanson, T. R. (2011). Effects of stocking density on the perfromance of pacific white shrimp Litopenaeus vannamei cultured under pond and outdoor tank conditions using a high soybean meal diet. Aquaculture, 319(1-2):232-239.
  31. Sulmartiwi, L., Rekasana, A., & Sudarno, S. (2013). Distribusi penyakit Infectious Myo Necrosis Virus (IMNV) pada udang vaname (Litopenaeus vannamei) di pantai Utara Jawa Timur. Jurnal Ilmiah Perikanan Dan Kelautan, 5(1):49-54.
  32. Sukenda, Nuryati, S., & Sari, I. R. (2010). Pemberian meniran Phyllanthus niruri untuk pencegahan infeksi IMNV (Infectious Myonecrosis Virus) pada udang vaname (Litopenaus vannamei). Undergraduate Thesis. Bogor: IPB University.
  33. Supriatna, Mahmudi, M., Musa, M., & Kusriani. (2020). Hubungan pH dengan parameter kualitas air pada tambak intensif udang vanamei (Litopenaus vannamei). Journal of Fisheries and Marine Research, 4(3):368-374.
  34. Syafaat, M. N., Gunarto, & Mansyur, A. (2013). Evaluasi kualitas air pada udang vaname (Litopenaeus vannamei) semi intensif dan intensif dengan aplikasi probiotik. Prosiding Forum Inovasi Teknologi Akuakultur:813-823.
  35. Syafaat, M. N., Mansyur, A., & Tonnek, S. (2010). Dinamika kualitas air pada budidaya udang vaname (Litopenaeus vannamei) semi-intensif dengan teknik pergiliran pakan. Prosiding Indoaqua-Forum Inovasi Teknologi Akuakultur:487–494.
  36. Tang, K. F. J., Melba, & Arthur, J. R. (2019). Shrimp Infectious Myonecrosis strategy manual. Rome: FAO.
  37. Tang, K. F. J., Pantoja, C. R., Poulos, B. T., Redman, R. M., & Lightnere, D. V. (2005). In situ hybridization demonstrates that Litopenaeus vannamei, L. stylirostris and Penaeus monodon are susceptible to experimental infection with Infectious Myonecrosis Virus (IMNV). Diseases of Aquatic Organisms, 63(2-3):261-265.
  38. Tobing, S. W. L. (2019). Pertumbuhan dan kelulushidupan udang vaname Litopenaeus vannamei pada salinitas 5 ppt dengan kepadatan berbeda. Thesis. Bandar Lampung: Lampung University.
  39. Umiliana, M., Sarjito, & Desrina. (2016). Pengaruh salinitas terhadap infeksi Infectious Myonecrosis Virus (IMNV) pada udang vaname Litopenaeus vannamei. Journal of Aquaculture Management And Technolo, 5(1):73-81.
  40. Wafi, A., Ariadi, H., Fadjar, M., Mahmudi, M., & Supriatna, S. (2020). Model simulasi panen parsial pada pengelolaan budidaya intensif udang vannamei (Litopenaeus vannamei). Jurnal Ilmu Perikanan, 11(2):118-126.
  41. Wang, L-U., & Chen, J-C. (2005). The immune response of white shrimp Litopenaeus vannamei and its susceptibility to Vibrio alginolyticus at different salinity levels. Fish and Shellfish Immunology, 18:269-278.
  42. Widanarni, W., Rahmi, D., Gustilatov, M., Sukenda, S., & Utami, D. A. S. (2020). Immune responses and resistance of white shrimp (Litopenaeus vannamei) fed probiotic Bacillus sp NP5 and prebiotic honey against White Spot Syndrome Virus infection. Jurnal Akuakultur Indonesia, 19(2):118-130.
  43. Yudiati, E. (2016). Ekspresi gen dan laju sintasan udang vaname (Litopenaeus vannamei) yang tersuplementasi dengan alginat secara oral untuk resistensi penyakit White Spot Syndrome Virus. Buletin Oseanografi Marina, 5(2):135-142.
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Author Biography

Nur Komariah Baladrat, Jakarta Technical University of Fisheries.
I'm a postgraduate student in 2019 majoring in aquaculture industry
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