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Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Molecular Genetics DNA Barcoding

DNA Barcoding on Indian Ocean Squid, Uroteuthis duvaucelii (D'Orbigny, 1835) (Cephalopoda: Loliginidae) from the Java Sea, Indonesia

https://doi.org/10.20473/jipk.v14i2.33448
Norma Afiati
Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, Central Java, 50275.
https://orcid.org/0000-0001-9788-4815
Subagiyo Subagiyo
Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, Central Java, 50275.
https://orcid.org/0000-0002-8446-9038
Christina Retna Handayani
Directorate for Aquaculture Development and Fish Health, Directorate General of Aquaculture, Ministry of Marine Affairs and Fisheries of the Republic of Indonesia, Central Jakarta, 10110.
https://orcid.org/0000-0003-4135-8546
Retno Hartati
Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, Central Java, 50275.
https://orcid.org/0000-0002-3131-5166
Nenik Kholilah
Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, Central Java, 50275.
https://orcid.org/0000-0002-8335-3498

Corresponding Author(s) : Norma Afiati

normaafiati.na@gmail.com

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Highlight Research

  1. The possibility of U. duvauceli be a polymorph cryptic species is further reinforced by the results of the present study.
  2. Phylogenetic analysis showed two separate clusters though did not perfectly segregate north to the south waters.
  3. Individuals in cluster II from the south coast showed the highest polymorphism levels by 40 segregating sites (S), compared to only 2-4 sites in that of cluster I
  4. Frequencies of A, C, G, and T in mitochondrial DNA of all specimens skewed toward A and T, with T being the most preferred and G being the least preferred nucleotide
  5. All specimens showed no amino acid frequency for glutamic acid. Further, all four individuals in cluster II (south coast) were also have no amino acid frequencies for aspartic acid and valine as well.


Abstract

Uroteuthis duvauceli (D' Orbigny, 1835) also known as the Indian Ocean Squid is a highly important commercial marine organism along the Java coast, Indonesia. Based on genetic variation this species-complex are polymorphic and cryptic. In the present study, the genetic diversity and stock structure of loliginid squid U. duvauceli is investigated using a mitochondrial gene cytochrome oxidase subunit I (COI). Samples were collected by hand-jigging onboard of an 8hp small fisher-boat equipped with a few lamps during May to August 2015, May 2016 and August to November 2018. Sample collection started at dusk until midnight. The attractor was a weighed-quill attached to nylon string, manually immersed into the water and pulled quickly and continuously for about 3-5 minutes at each effort. The determination was conducted with BLAST. Phylogenetic analysis showed two separate clusters with 100% bootstrap value, in which cluster II from Palabuhanratu has divergences of 5.9 - 7.0%, compared to cluster I.  Genetic variations exist within and among individuals over the locations. Palabuhanratu individuals have the highest polymorphism levels compared to other locations as shown by 40 segregating sites (S). Frequencies of A, C, G, and T in mtDNA of the 20 specimens U. duvauceli are biased toward A and T, with T being the most favoured and G being the least favoured nucleotide. All specimen showed no amino acid frequency for glutamic acid and all four individuals in cluster II (south coast) also have no amino acid frequencies for aspartic acid and valine as well.

Keywords

Uroteuthis duvauceli mtDNA COI Java Sea Indonesia
Norma Afiati, Subagiyo, S., Handayani, C. R., Hartati, R., & Kholilah, N. (2022). DNA Barcoding on Indian Ocean Squid, <I>Uroteuthis duvaucelii</I> (D’Orbigny, 1835) (Cephalopoda: Loliginidae) from the Java Sea, Indonesia. Jurnal Ilmiah Perikanan Dan Kelautan, 14(2), 231–245. https://doi.org/10.20473/jipk.v14i2.33448
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Author Biographies

Norma Afiati, Department of Aquatic Resources Management, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, Central Java, 50275.

Dept. of Aquatic Resources Management,

Professor.

Subagiyo Subagiyo, Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, Central Java, 50275.
Department of Marine Science, Lecturer
Christina Retna Handayani, Directorate for Aquaculture Development and Fish Health, Directorate General of Aquaculture, Ministry of Marine Affairs and Fisheries of the Republic of Indonesia, Central Jakarta, 10110.
Research Centre for Brackish Water Aquaculture Development, Researcher
Retno Hartati, Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, Central Java, 50275.
Department of Marine Science, Lecturer
Nenik Kholilah, Department of Marine Science, Faculty of Fisheries and Marine Science, Universitas Diponegoro, Semarang, Central Java, 50275.
Department of Marine Science, Academic Staff.
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