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Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Aquaculture Fish Diseases Fish Health Management

Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila

https://doi.org/10.20473/jipk.v16i1.44079
Arini Resti Fauzi
Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-0162-6895
Munti Yuhana
Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-4019-0721
Widanarni Widanarni
Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-0821-3225
Mia Setiawati
Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-6604-8184
Usamah Afiff
Department of Veterinary Disease and Public Health, Veterinary and Biomedical School, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-1373-9520

Corresponding Author(s) : Munti Yuhana

muntiyu@apps.ipb.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract


Abstract


Dayak onion (Eleutherine bulbosa (Mill.) Urb.) has the potential to be an immunostimulant to benefit fish health. This study aimed to evaluate the effectiveness of dietary supplementation with the powder and crude ethanol extracts of Dayak onion on the hematology and immune response parameters of Nile tilapia challenged with Aeromonas hydrophila. The research used a completely randomized design with six treatments and three replications. Nile tilapias were fed with Dayak onion (w/v) consisting of powders of 5% (P5), 10% (P10), and 15% (P15), while crude extract was 0.5% (E05) and given during rearing for 30 days. Positive control (C+) and negative control (C-) were used without Dayak onion. Nile tilapia in treatment C+, P5, P10, and P15 were injected with A. hydrophila 10ⶠCFU mLâ»1, while that in treatment C- was injected with phosphate buffered saline (PBS) intramuscularly. The results showed that the dietary with 15% powder and 0.5% crude extract significantly improved the hematology and immune response parameters compared to the control after the challenge against A. hydrophila (P<0.05). Dayak onion supplementation effectively increased the health status based on hematology and immune response parameters of Nile tilapia against A. hydrophila infection.


Highlight Research



  1. Dayak onion has significant potential to improve nonspecific immunity in fish.

  2. Dayak onion improves hematology and immune response of Nile tilapia.

  3. Dayak onion can significantly regulate the immune system against A. hydrophila infection.

  4. Dayak onion significantly increases the production of the gene expression of IL-1β and TNF-α.

Keywords

Eleutherine bulbosagene expressionHistopathologyImmunostimulant
Fauzi, A. R., Yuhana, M., Widanarni, W., Setiawati, M., & Afiff, U. (2023). Effect of Dayak Onion (Eleutherine bulbosa (Mill.) Urb.) on the Immune Response and Gene Expression of Nile Tilapia (Oreochromis niloticus) Infected with Aeromonas hydrophila. Jurnal Ilmiah Perikanan Dan Kelautan, 16(1), 1–14. https://doi.org/10.20473/jipk.v16i1.44079
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Author Biographies

Arini Resti Fauzi, Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Munti Yuhana, Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Widanarni Widanarni, Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Mia Setiawati, Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java. Indonesia

Usamah Afiff, Department of Veterinary Disease and Public Health, Veterinary and Biomedical School, IPB University, Bogor, West Java. Indonesia

Department of Veterinary Disease and Public Health, Veterinary and Biomedical School, IPB University, Bogor, West Java. Indonesia

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